Exogenous Protein as an Environmental Stimuli of Biofilm Formation in Select Bacterial Strains

Ye, Donna; Bapu, Lekha; Cavalcante, Mariane Mota; Kato, Jesse; Sneideman, Maggie Lauria; Scribner, Kim T.; Loch, Thomas P.; Marsh, Terence L.

doi:10.1101/683979

Cited by 1 publication

(1 citation statement)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This strain was used as a strong positive control for biofilm formation. Additional isolates in this study were purified from the eggs of lake sturgeon (42), or in the case of Serratia marcescens RL-10, from soil (44). Isolates from the sturgeon egg were initially identified with partial 16S rRNA sequencing.…”

Section: Strains Media and Cultivationmentioning

confidence: 99%

The impact of primary colonizers on the community composition of river biofilm

Angoshtari

Scribner

Marsh

2022

Preprint

Self Cite

View full text Add to dashboard Cite

As a strategy for minimizing microbial infections in fish hatcheries, we have investigated how putatively probiotic bacterial populations influence biofilm formation on the surface of eggs. All surfaces that are exposed to the aquatic milieu develop a microbial community through the selective assembly of microbial populations into a surface-adhering biofilm. In the investigations reported herein, we describe laboratory experiments designed to determine how initial colonization of a surface by nonpathogenic isolates from sturgeon eggs influence the subsequent assembly of populations into the existing biofilm from a pelagic river community. All eight of the tested strains altered the assembly of river biofilm in a strain-specific manner. Previously formed isolate biofilm was challenged with natural river populations and after 24 hours, two strains and two isolate combinations proved highly resistant to invasion, comprising at least 80% of the biofilm community, four isolates were intermediate in resistance with at least 45% of the biofilm community and two isolates were reduced to 4% of the biofilm community. Founding biofilms of Serratia sp, and combinations of Brevundimonas sp.-Hydrogenophaga sp. and Brevundimonas sp.-Acidovorax sp. specifically blocked populations of Aeromonas and Flavobacterium, potential fish pathogens, from colonizing the biofilm. In addition, all isolate biofilms were effective at blocking invading populations of Arcobacter. Several strains, notably Deinococcus sp., recruited specific river populations into the biofilm. The experiments suggest that relatively simple measures can be used to control the assembly of biofilm on the eggs surface and perhaps offer protection from pathogens. In addition, the methodology provides a relatively rapid way to detect potentially strong ecological interactions between bacterial populations in the formation of biofilms.

show abstract

Section: Strains Media and Cultivationmentioning

confidence: 99%