Exogenous silicon relieve drought stress and salt stress of <i>Glycyrrhiza uralensis</i> seedlings by regulating proline metabolism and nitrogen assimilation

Cui, Gaochang; Xiao, Xiang; Zhang, Wenjin; Lang, Duoyong; Li, Zhixian; Zhang, Xinhui

doi:10.1080/14620316.2021.1921624

Cited by 17 publications

(10 citation statements)

References 58 publications

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“…Although, in our study was verified that higher contents of proline than a critical point might lead to negative effect on the plants. In concordance with our study, different authors showed that proline regulation application was able to decrease the negative effects of environmental stress on Trifolium repens L. 30 , tobacco 32 , Glycyrrhiza uralensis 27 , and chickpea 28 mostly via decreasing the content of H 2 O 2 and malondialdehyde and also increasing the activity of enzymatic antioxidants. Also, Zali and Ehsanzadeh 33 and 34 Showed that proline was capable of increasing the growth of fennel And Phaseolus vulgaris under water deficit conditions and nutrient deficiency.…”

Section: Discussionsupporting

confidence: 92%

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Alternate gene expression profiling of monoterpenes in Hymenocrater longiflorus as a novel pharmaceutical plant under water deficit

Saed-Moucheshi

Mozafari

2022

Sci Rep

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Hymenocrater longiflorus (surahalala) is a wild plant species with potential pharmaceutical and ornamental interest. To date, the genomics of this plant is unknown and the gene expression profiling of the genes related to its metabolite has never been studied before. In order to study the responses of in vitro-grown surahalala plants to abiotic stresses and the differential expression of the genes related to its essential oils under exogenous proline application; three levels of PEG600 (0, 10, and 20%) and five levels of proline (0, 5, 10, 15, and 20 µm) were combined in the culture media. Thus, water deficit increased oxidants levels and decreased fresh weight of surahalala tissues, whereas addition of proline up to 15 µm was able to relatively compensate the negative effect of water deficit. Contrarily, high proline level (20 µm) had a negative effect on surahalala plants probably due to the stress simulation (nutrition) under high proline concentration. In addition, the best combination for achieving highest essential oils content was 10 µm proline plus 10% PEG. The expressional profiling of the genes TPS27, L3H, TPS2, TPS1, OMT and GDH3 were successfully carried out and their involvement in 1,8-cineole, carvone, α-pinene, thymol, estragole and β-Citronellol biosynthesis, respectively, was verified. In addition, our results indicated that these genes could also be involved in the synthesis of other metabolites under water deficit condition.

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Section: Discussionsupporting

confidence: 92%

“…In most of cases application exogenous proline led to higher tolerant in plants under water deficit stress. However, its application has sowed different effects on the contents of secondary metabolites and the expressional levels of their related genes 27 – 32 .…”

Section: Discussionmentioning

confidence: 99%

Alternate gene expression profiling of monoterpenes in Hymenocrater longiflorus as a novel pharmaceutical plant under water deficit

Saed-Moucheshi

Mozafari

2022

Sci Rep

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“…Altogether, our results coincide with the findings of several authors: Danish and Zafar-ul-Hye (2019) showed biochar and PGPR co-application improved yield of wheat under drought stress, Asaf et al (2018) reported GA producing fungus Aspergillus flavus conferred salt tolerance in Glycine max . Similarly, Biju et al (2017) and Cui et al (2021) reported Si application alleviated drought stress in crops by regulating enzymes, osmolytes, oxidative metabolism, and nutrient assimilation. Adnan et al (2020) and Kaya et al (2003) showed the supplementation of potassium and phosphorus coupled with phosphate solubilizing bacteria ameliorated salt stress in maize, pepper and cucumber plants.…”

Section: Discussionmentioning

confidence: 93%

The Combined Inoculation of Curvularia lunata AR11 and Biochar Stimulates Synthetic Silicon and Potassium Phosphate Use Efficiency, and Mitigates Salt and Drought Stresses in Rice

et al. 2022

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Synthetic chemical fertilizers are a fundamental source of nutrition for agricultural crops; however, their limited availability, low plant uptake, and excessive application have caused severe ecological imbalances. In addition, the gravity of environmental stresses, such as salinity and water stress, has already exceeded the threshold limit. Therefore, the optimization of nutrient efficiency in terms of plant uptake is crucial for sustainable agricultural production. To address these challenges, we isolated the rhizospheric fungus Curvularia lunata ARJ2020 (AR11) and screened the optimum doses of biochar, silicon, and potassium phosphate (K2HPO4), and used them—individually or jointly—to treat rice plants subjected to salt (150 mM) and drought stress (20–40% soil moisture). Bioassay analysis revealed that AR11 is a highly halotolerant and drought-resistant strain with an innate ability to produce gibberellin (GA1, GA3, GA4, and GA7) and organic acids (i.e., acetic, succinic, tartaric, and malic acids). In the plant experiment, the co-application of AR11 + Biochar + Si + K2HPO4 significantly improved rice growth under both salt and drought stresses. The plant growth regulator known as abscisic acid, was significantly reduced in co-application-treated rice plants exposed to both drought and salt stress conditions. These plants showed higher Si (80%), P (69%), and K (85%) contents and a markedly low Na+ ion (208%) concentration. The results were further validated by the higher expression of the Si-carrying gene OsLSi1, the salt-tolerant gene OsHKT2, and the OsGRAS23’s drought-tolerant transcriptome. Interestingly, the beneficial effect of AR11 was significantly higher than that of the co-application of Biochar + Si + K2HPO4 under drought. Moreover, the proline content of AR11-treated plants decreased significantly, and an enhancement of plant growth-promoting characteristics was observed. These results suggest that the integrated co-application of biochar, chemical fertilizers, and microbiome could mitigate abiotic stresses, stimulate the bioavailability of essential nutrients, relieve phytotoxicity, and ultimately enhance plant growth.

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“…P5CS is a key rate-limiting enzyme involved in the glutamate synthesis pathway of proline, and its activity is feedback inhibited by proline (Meena et al, 2019). Cui et al (2021) found that P5CS was significantly upregulated in Glycyrrhiza uralensis under drought conditions. Similarly, Ghaffar et al (2021) found that PRODH was significantly downregulated in B. napus.…”

Section: Discussionmentioning

confidence: 95%

Transcriptomic and Metabolomic Analyses Reveal Key Metabolites, Pathways and Candidate Genes in Sophora davidii (Franch.) Skeels Seedlings Under Drought Stress

et al. 2022

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Soil aridification and desertification are particularly prominent in China’s karst areas, severely limiting crop yields and vegetation restoration. Therefore, it is very important to identify naturally drought-tolerant plant species. Sophora davidii (Franch.) Skeels is resistant to drought and soil infertility, is deeply rooted and is an excellent plant material for soil and water conservation. We studied the transcriptomic and metabolomic changes in S. davidii in response to drought stress (CK, control; LD, mild drought stress; MD, moderate drought stress; and SD, severe drought stress). Sophora davidii grew normally under LD and MD stress but was inhibited under SD stress; the malondialdehyde (MDA), hydrogen peroxide (H2O2), soluble sugar, proline, chlorophyll a, chlorophyll b and carotenoid contents and ascorbate peroxidase (APX) activity significantly increased, while the superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) activities and soluble protein content significantly decreased. In the LD/CK, MD/CK and SD/CK comparison groups, there were 318, 734 and 1779 DEGs, respectively, and 100, 168 and 281 differentially accumulated metabolites, respectively. Combined analysis of the transcriptomic and metabolomic data revealed the metabolic regulation of S. davidii in response to drought stress. First, key candidate genes such as PRR7, PRR5, GI, ELF3, PsbQ, PsaK, INV, AMY, E2.4.1.13, E3.2.1.2, NCED, PP2C, PYL, ABF, WRKY33, P5CS, PRODH, AOC3, HPD, GPX, GST, CAT and SOD1 may govern the drought resistance of S. davidii. Second, three metabolites (oxidised glutathione, abscisic acid and phenylalanine) were found to be related to drought tolerance. Third, several key candidate genes and metabolites involved in 10 metabolic pathways were identified, indicating that these metabolic pathways play an important role in the response to drought in S. davidii and possibly other plant species.

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Exogenous silicon relieve drought stress and salt stress of Glycyrrhiza uralensis seedlings by regulating proline metabolism and nitrogen assimilation

Cited by 17 publications

References 58 publications

Alternate gene expression profiling of monoterpenes in Hymenocrater longiflorus as a novel pharmaceutical plant under water deficit

Alternate gene expression profiling of monoterpenes in Hymenocrater longiflorus as a novel pharmaceutical plant under water deficit

The Combined Inoculation of Curvularia lunata AR11 and Biochar Stimulates Synthetic Silicon and Potassium Phosphate Use Efficiency, and Mitigates Salt and Drought Stresses in Rice

Transcriptomic and Metabolomic Analyses Reveal Key Metabolites, Pathways and Candidate Genes in Sophora davidii (Franch.) Skeels Seedlings Under Drought Stress

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