Exogenous spermidine improves seed germination of sweet corn via involvement in phytohormone interactions, H2O2 and relevant gene expression

Huang, Yue; Lin, Chi-Chung; He, Fei; Zhan, Li; Guan, Yajing; Hu, Qijuan; Hu, Jin

doi:10.1186/s12870-016-0951-9

Cited by 238 publications

(182 citation statements)

References 55 publications

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“…This increase could be one of the reasons for the lowest pigeonpea germination rates when using 0.5 mM Spd ( Figure 1C). Moreover, exogenous Spd might have inhibited the stimulatory effects of plant hormones that participate in seed germination, such as gibberellins (GA) and ethylene (ET), or it could have stimulated abscisic acid (ABA), which is a powerful inhibitor of seed germination (Huang et al, 2017). The decrease in the germination rates of both species under salt stress caused by 0.5 mM SA and Spd might have occurred due to the effect of the low or slightly moderate NaCl concentrations used in this study (20 mM -60 mM).…”

Section: Resultsmentioning

confidence: 99%

“…In fact, the role of SA may vary with the stress level, i.e., moderate or severe, even if there is an interaction between the downstream signals of ROS and SA, in which redox regulation plays a key role (Barba-Espín et al, 2011). Nevertheless, the concentration of 0.5 mM SA or Spd might have altered the ratio of gibberellin (GA) to abscisic acid (ABA) in the pigeonpea and lab lab under saline stress; these are hormones that modulate seed germination, so when GA is suppressed, germination is stimulated, or when ABA is induced, germination is inhibited (Rajjou et al, 2006;Tan et al, 2013;Huang et al, 2017). The germination inhibition by exogenous SA could be based on oxidative stress.…”

Section: Resultsmentioning

confidence: 99%

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Pretreatment of forage legumes under moderate salinity with exogenous salicylic acid or spermidine

et al. 2020

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The present study aims to determine whether exogenous salicylic acid (SA) or spermidine (Spd) has any protective effect against salt stress. Seeds were subjected to 0, 20, 40, and 60 mM NaCl with or without salicylic acid or spermidine (0.5 mM) for 10 days. The evaluated variables were germination rate, shoot and root dry masses, glycine betaine content, lipid peroxidation, and the activities of superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX). The data were subjected to Tukey’s test (p ≤ 0.05). There was a growth increase, especially in plant shoots. The reduction in lipid peroxidation, as indicated by lower malondialdehyde (MDA) levels, can be explained by an increase in antioxidant activity when SA and Spd were added. When compared to CAT and APX, SOD was the least responsive enzyme to the addition of both SA and Spd in salt-stressed plants. SA and Spd partially reduced the effects of moderate salt stress in both plant species; however, Spd addition had better results than SA in terms of suppressing oxidative stress. Lablab plants were more vigorous than pigeonpea plants.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Pretreatment of forage legumes under moderate salinity with exogenous salicylic acid or spermidine

et al. 2020

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“…Previous studies have also reported the positive impacts on seed germination through Spd priming (Rebecca et al, 2010;Sedagahat and Rahemi, 2011). Recently, it has been shown that Spd soaking can significantly improve seed germination in corn, while exogenous application of cyclohexylamine (CHA; an inhibitor of Spd biosynthesis) resulted in significant inhibition of seed germination and declined seed vigour (Huang et al, 2017). In comparison with control, seeds pre-soaking in polyamine solutions drastically improved the seedling growth.…”

Section: Resultsmentioning

confidence: 99%

EFFECT OF POLYAMINES APPLICATION ON GERMINATION AND PHYSIOLOGICAL CHARACTERISTICS OF BORAGE (Borago officinalis L.)

Danalo¹,

Shekari²,

Sabaghnia³

et al. 2018

AgricultForest

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The aim of study was determine the advantages of re-drying after seed priming by polyamines. As biologically active compounds, polyamines (PAs) have been considered as modulator of plant growth and development, they play a significant role in plant response to environmental stress. The effects of polyamines priming on seed germination, emergence and seedling growth of borage plants was investigated by a laboratory experiment in factorial layout with complete randomized design (CRD) conducted in three replications. The seeds were classified into five sub-samples one of which was kept as control (unprimed) while the rest of them were primed with polyamines. Seeds pretreatments included: control (unprimed), water pretreatment for 4 and 8 h, spermidine at 5 and 5.5 mM for 4 and 8 h, spermine at 2.5 and 3 mM for 4 and 8 h, putrescine at 2.5 and 3 mM for 4 and 8 h. Sseed treatments with polyamine led to earlier and enhanced germination. Improved seedling length, seedling fresh and dry weight as well as vigor index were found in polyamine-treated seeds. Moreover, the majority of priming treatments enhanced seedling emergence percentage, emergence energy and coefficient of uniformity of emergence (CUE) as compared with control samples. Non-primed seeds (control samples) significantly showed the least α-amylase and β-amylase activity (0.293 and 4.923 U.mg -1 Protein, respectively ) and shortest of plant height. 4-hour seed treatment by 3mM putrescine and 8-hour treatment with 3mM spermine were recognized as the most effective treatments in most of the studied traits.

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“…In our study, DEGs involved in SAMDC and SPMS were expressed in both cultivars, but higher expression levels were observed in KN27-3 than in XLZ38 during seed germination. Multiple studies have demonstrated that Spd is generally correlated with the hypocotyl elongation rate, such as in chickpea [46], corn [47], and in mung bean [48]. Thus, higher expression of SAMDC and SPMS may increase the accumulation of Spd in germinating KN27-3 seeds under chilling conditions.…”

Section: Discussionmentioning

confidence: 98%

Comparative Transcriptome Analysis Provides Insights into the Seed Germination in Cotton in Response to Chilling Stress

Shen

Zhang

Liu

et al. 2020

IJMS

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Gossypium hirsutum L., is a widely cultivated cotton species around the world, but its production is seriously threatened by its susceptibility to chilling stress. Low temperature affects its germination, and the underlying molecular mechanisms are rarely known, particularly from a transcriptional perspective. In this study, transcriptomic profiles were analyzed and compared between two cotton varieties, the cold-tolerant variety KN27-3 and susceptible variety XLZ38. A total of 7535 differentially expressed genes (DEGs) were identified. Among them, the transcripts involved in energy metabolism were significantly enriched during germination based on analysis of Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, such as glycolysis/gluconeogenesis, tricarboxylic acid cycle (TCA cycle), and glyoxylate cycle (GAC). Results from further GO enrichment analysis show the earlier appearance of DNA integration, meristem growth, cotyledon morphogenesis, and other biological processes in KN27-3 compared with XLZ38 under chilling conditions. The synthesis of asparagine, GDP-mannose, and trehalose and the catabolic process of raffinose were activated. DEGs encoding antioxidants (spermidine) and antioxidase (CAT1, GPX4, DHAR2, and APX1) were much more up-regulated in embryos of KN27-3. The content of auxin (IAA), cis-zeatin riboside (cZR), and trans-zeatin riboside (tZR) in KN27-3 are higher than that in XLZ38 at five stages (from 12 h to 54 h). GA3 was expressed at a higher level in KN27-3 from 18 h to 54 h post imbibition compared to that in XLZ38. And abscisic acid (ABA) content of KN27-3 is lower than that in XLZ38 at five stages. Results from hormone content measurements and the related gene expression analysis indicated that IAA, CTK, and GA3 may promote germination of the cold-tolerant variety, while ABA inhibits it. These results expand the understanding of cottonseed germination and physiological regulations under chilling conditions by multiple pathways.

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Exogenous spermidine improves seed germination of sweet corn via involvement in phytohormone interactions, H2O2 and relevant gene expression

Cited by 238 publications

References 55 publications

Pretreatment of forage legumes under moderate salinity with exogenous salicylic acid or spermidine

Pretreatment of forage legumes under moderate salinity with exogenous salicylic acid or spermidine

EFFECT OF POLYAMINES APPLICATION ON GERMINATION AND PHYSIOLOGICAL CHARACTERISTICS OF BORAGE (Borago officinalis L.)

Comparative Transcriptome Analysis Provides Insights into the Seed Germination in Cotton in Response to Chilling Stress

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