Exogenous strigolactone interacts with abscisic acid-mediated accumulation of anthocyanins in grapevine berries

Ferrero, Manuela; Pagliarani, Chiara; Novák, Ondřej; Ferrandino, Alessandra; Cardinale, Francesca; Visentin, Ivan; Schubert, Andrea

doi:10.1093/jxb/ery033

Cited by 65 publications

(44 citation statements)

References 76 publications

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“…Finally, independently of the cultivar, many transporter-encoding genes were transcriptionally activated in PM-infected leaves following treatment with AcS-Mt. These included ABC and ABCG transporters, such as the Vitis homolog to the Arabidopsis abscisic acid (ABA) transporter ABCG40 [ 42 ], amino acid permeases, and different genes involved in the transport of sugars, such as the grape hexose transporters VvHT 2 and VvHT5 [ 43 ], and glucose-6-phosphate/phosphate translocators ( Table S3 ). Accordingly, results achieved through candidate gene expression analysis attested that invertase genes, such as the cell wall invertase VvCW-INV ( Figure 2 g) and the acidic vacuolar invertase VvGIN2 ( Figure S2c ), were strongly induced upon AcS-Mt treatment, as well as sugar transport transcripts ( VvHT5 , Figure 2 h and VvHT2 , Figure S2d ).…”

Section: Resultsmentioning

confidence: 99%

The Molecular Priming of Defense Responses is Differently Regulated in Grapevine Genotypes Following Elicitor Application against Powdery Mildew

Pagliarani

Moine

Chitarra

et al. 2020

IJMS

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Molecular changes associated with response to powdery mildew (PM) caused by Erysiphe necator have been largely explored in Vitis vinifera cultivars, but little is known on transcriptional and metabolic modifications following application of resistance elicitors against this disease. In this study, the whole transcriptome sequencing, and hormone and metabolite analyses were combined to dissect long-term defense mechanisms induced by molecular reprogramming events in PM-infected ‘Moscato’ and ‘Nebbiolo’ leaves treated with three resistance inducers: acibenzolar-S-methyl, potassium phosphonate, and laminarin. Although all compounds were effective in counteracting the disease, acibenzolar-S-methyl caused the most intense transcriptional modifications in both cultivars. These involved a strong down-regulation of photosynthesis and energy metabolism and changes in carbohydrate accumulation and partitioning that most likely shifted the plant growth-defense trade-off towards the establishment of disease resistance processes. It was also shown that genotype-associated metabolic signals significantly affected the cultivar defense machinery. Indeed, ‘Nebbiolo’ and ‘Moscato’ built up different defense strategies, often enhanced by the application of a specific elicitor, which resulted in either reinforcement of early defense mechanisms (e.g., epicuticular wax deposition and overexpression of pathogenesis-related genes in ‘Nebbiolo’), or accumulation of endogenous hormones and antimicrobial compounds (e.g., high content of abscisic acid, jasmonic acid, and viniferin in ‘Moscato’).

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Section: Resultsmentioning

confidence: 99%

The Molecular Priming of Defense Responses is Differently Regulated in Grapevine Genotypes Following Elicitor Application against Powdery Mildew

Pagliarani

Moine

Chitarra

et al. 2020

IJMS

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“…Interestingly, a sugar-mediated regulation does exist on both structural genes of flavonoid biosynthesis and transcription factors involved in the anthocyanin pathway (Lecourieux et al, 2014). Moreover, ABA metabolic pathways are strictly associated with changes in sugar turnover (Hayes et al, 2010) and anthocyanin production in the berry (Ferrero et al, 2018). Consistently, the ABA biosynthetic gene VvNCED1 (VIT_19s0093g00550) was overexpressed at the onset of ripening in both the experimental years, in line with the patterns of ABA concentrations ( Figure S7 ) and with the transcriptional profiles of HT2 and HT6 ( Figures S6B, C ) and of the anthocyanin biosynthetic genes UFGT and AOMT ( Figures 6C and S8 ).…”

Section: Resultsmentioning

confidence: 99%

Distinct Metabolic Signals Underlie Clone by Environment Interplay in “Nebbiolo” Grapes Over Ripening

et al. 2019

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Several research studies were focused to understand how grapevine cultivars respond to environment; nevertheless, the biological mechanisms tuning this phenomenon need to be further deepened. Particularly, the molecular processes underlying the interplay between clones of the same cultivar and environment were poorly investigated. To address this issue, we analyzed the transcriptome of berries from three "Nebbiolo" clones grown in different vineyards, during two ripening seasons. RNA-sequencing data were implemented with analyses of candidate genes, secondary metabolites, and agronomical parameters. This multidisciplinary approach helped to dissect the complexity of clone × environment interactions, by identifying the molecular responses controlled by genotype, vineyard, phenological phase, or a combination of these factors. Transcripts associated to sugar signalling, anthocyanin biosynthesis, and transport were differently modulated among clones, according to changes in berry agronomical features. Conversely, genes involved in defense response, such as stilbene synthase genes, were significantly affected by vineyard, consistently with stilbenoid accumulation. Thus, besides at the cultivar level, clone-specific molecular responses also contribute to shape the agronomic features of grapes in different environments. This reveals a further level of complexity in the regulation of genotype × environment interactions that has to be considered for orienting viticultural practices aimed at enhancing the quality of grape productions.

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“…Secondly, under irrigated conditions, miR156-oe plants had lower stomatal conductance but also significantly less free ABA in leaves, possibly due to weaker biosynthesis and especially accelerated ABA conversion to phaseic acid. Accordingly, the activation of ABA catabolism is a reported effect of increased strigolactone synthesis in the shoot, and of exogenous strigolactone treatment in different tissues (Ferrero et al, 2018;Lechat et al, 2012;Toh et al, 2012;Visentin et al, 2016). Thirdly, stomatal closure by exogenous ABA was more complete in miR156-oe plants than in wt, which may be explained by higher sensitivity to, or more effective transport of ABA.…”

Section: Mir156 Is a Possible Mediator Of Aba-dependent Effects Of Strigolactones On Stomatamentioning

confidence: 99%

A novel strigolactone‐miR156 module controls stomatal behaviour during drought recovery

Visentin

Pagliarani

Deva

et al. 2020

Plant Cell & Environment

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The Ministry of Education, Youth and Sports, Czech Republic (Grant LO1204 from the National Programme of Sustainability I) supported V.T. and O.N. The PlantStressLab team and E.D. have received funding from the European Union's Horizon 2020 research and innovation programme under the Grant Agreement No. [727929] (TOMRES). None of these funding sources were involved in the design of the study and collection, analysis and interpretation of data, and in writing the manuscript.F.C. agrees to serve as the author responsible for contact and ensures communication. The Ministry of Education, Youth and Sports, Czech Republic (Grant LO1204 from the National Programme of Sustainability I) supported V.T. and O.N. The PlantStressLab team and E.D. have received funding from the European Union's Horizon 2020 research and innovation programme under the Grant Agreement No. [727929] (TOMRES). None of these funding sources were involved in the design of the study and collection, analysis and interpretation of data, and in writing the manuscript. The authors wish to thank Dr. Silva e Ferreira (Sao Paulo, Brazil) for sharing the miR156-oe MicroTom line, Dr. Klee (University of Florida, USA) for the strigolactone-depleted line, and Viviana Cisse and Cecilia Roche for technical support. Word count, main body (Introduction to Acknowledgements): 5976 Number of figures: 6, of which 0 in colour Number of supplementary figures: 1 of which 0 in colour Number of supplementary tables: 1 Main text A novel strigolactone-miR156 module controls stomatal behaviour during drought recovery ABSTRACT miR156 is a conserved microRNA whose role and induction mechanisms under stress are poorly known. Strigolactones are phytohormones needed in shoots for drought acclimation. They promote stomatal closure ABA-dependently and independently; however, downstream effectors for the former have not been identified. Linkage between miR156 and strigolactones under stress has not been reported.We compared ABA accumulation and sensitivity as well as performances of wt and miR156overexpressing (miR156-oe) tomato plants during drought. We also quantified miR156 levels in wt, strigolactone-depleted and strigolactone-treated plants, exposed to drought stress.Under irrigated conditions, miR156 overexpression and strigolactone treatment led to lower stomatal conductance and higher ABA sensitivity. Exogenous strigolactones were sufficient for miR156 accumulation in leaves, while endogenous strigolactones were required for miR156 induction by drought. The "after-effect" of drought, by which stomata do not completely re-open after rewatering, was enhanced by both strigolactones and miR156. The transcript profiles of several miR156 targets were altered in strigolactone-depleted plants.Our results show that strigolactones act as a molecular link between drought and miR156 in tomato, and identify miR156 as a mediator of ABA-dependent effect of strigolactones on the aftereffect of drought on stomata. Thus, we provide insights into both strigolactone and miR156 action on stomata.

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Exogenous strigolactone interacts with abscisic acid-mediated accumulation of anthocyanins in grapevine berries

Abstract: The strigolactone analogue GR24 reduces ABA-induced anthocyanin accumulation in Vitis vinifera berries. GR24 treatment does not affect ABA biosynthesis while it activates ABA degradation and possibly ABA membrane transport.

Cited by 65 publications

References 76 publications

The Molecular Priming of Defense Responses is Differently Regulated in Grapevine Genotypes Following Elicitor Application against Powdery Mildew

The Molecular Priming of Defense Responses is Differently Regulated in Grapevine Genotypes Following Elicitor Application against Powdery Mildew

Distinct Metabolic Signals Underlie Clone by Environment Interplay in “Nebbiolo” Grapes Over Ripening

A novel strigolactone‐miR156 module controls stomatal behaviour during drought recovery

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