Exogenous Vasohibin-2 Exacerbates Angiotensin II-Induced Ascending Aortic Dilation in Mice

Okuyama, Michihiro; Uchida, Haruhito A.; Hada, Yoshiko; Kakio, Yuki; Otaka, Nozomu; Umebayashi, Ryoko; Tanabe, Katsuyuki; Fujii, Yasuhiro; Kasahara, Shingo; Subramanian, Venkateswaran; Daugherty, Alan; Sato, Yasufumi; Wada, Jun

doi:10.1253/circrep.cr-19-0008

Cited by 8 publications

(11 citation statements)

References 46 publications

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“…H-1705-0100, Switzerland) was infused via Alzet mini-osmotic pumps (Alzet, Model 2002, Cupertino, CA, USA) for 14 days. Mini-osmic pumps were implanted subcutaneously on the right flank, as described previously [ 52 , 53 ]. In the low and high CGA (Sigma-Aldrich, Cat.…”

Section: Methodsmentioning

confidence: 99%

“…clone N45.1, Shizuoka, Japan). Reactivity of the antibodies with tissue antigens was detected using AEC and ImmPACT AEC HRP Substrate (Vector Laboratories, SK-4200, Burlingame, CA, USA) as described previously [ 52 , 53 ].…”

Section: Methodsmentioning

confidence: 99%

“…no. WBLUC0100, Burlington, MA, USA) and were quantified using a General Electric Imager (GE Healthcare, LAS 4000 mini, Chicago, IL, USA) [ 52 , 53 ].…”

Section: Methodsmentioning

confidence: 99%

“…Saline or AngII (1000 ng/kg/min) was infused via Alzet mini-osmotic pumps for 14 days. Mini-osmic pumps were implanted subcutaneously on the right flank, as described previously [ 52 , 53 ]. In the CGA group, the mice were administered 40 mg/kg/day CGA via oral gavage for 14 days from the initial day of AngII infusion.…”

Section: Methodsmentioning

confidence: 99%

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The Protective Effect of Chlorogenic Acid on Vascular Senescence via the Nrf2/HO-1 Pathway

Hada

Uchida

Otaka

et al. 2020

IJMS

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The world faces the serious problem of aging. In this study, we aimed to investigate the effect of chlorogenic acid (CGA) on vascular senescence. C57/BL6 female mice that were 14 ± 3 months old were infused with either Angiotensin II (AngII) or saline subcutaneously for two weeks. These mice were administered CGA of 20 or 40 mg/kg/day, or saline via oral gavage. AngII infusion developed vascular senescence, which was confirmed by senescence associated-β-galactosidase (SA-β-gal) staining. CGA administration attenuated vascular senescence in a dose-dependent manner, in association with the increase of Sirtuin 1 (Sirt1) and endothelial nitric oxide synthase (eNOS), and with the decrease of p-Akt, PAI-1, p53, and p21. In an in vitro study, with or without pre-treatment of CGA, Human Umbilical Vein Endothelial Cells (HUVECs) were stimulated with H2O2 for an hour, then cultured in the absence or presence of 0.5–5.0 μM CGA for the indicated time. Endothelial cell senescence was induced by H2O2, which was attenuated by CGA treatment. Pre-treatment of CGA increased Nrf2 in HUVECs. After H2O2 treatment, translocation of Nrf2 into the nucleus and the subsequent increase of Heme Oxygenase-1 (HO-1) were observed earlier in CGA-treated cells. Furthermore, the HO-1 inhibitor canceled the beneficial effect of CGA on vascular senescence in mice. In conclusion, CGA exerts a beneficial effect on vascular senescence, which is at least partly dependent on the Nuclear factor erythroid 2-factor 2 (Nrf2)/HO-1 pathway.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…no. WBLUC0100, Burlington, MA, USA) and were quantified using a General Electric Imager (GE Healthcare, LAS 4000 mini, Chicago, IL, USA) [ 52 , 53 ].…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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The Protective Effect of Chlorogenic Acid on Vascular Senescence via the Nrf2/HO-1 Pathway

Hada

Uchida

Otaka

et al. 2020

IJMS

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“…no. WBLUC0100) and quantified using a General Electric Imager (GE Healthcare, LAS 4000 mini)[29].2.6. Gelatin Zymography.…”

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confidence: 99%

Fisetin Attenuates Lipopolysaccharide-Induced Inflammatory Responses in Macrophage

Hada

Uchida

Wada

2021

BioMed Research International

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Several studies have reported the efficacy and safety of polyphenols in human health; however, the verification of their efficacy remains insufficient. The aim of this study was to examine whether fisetin, one of flavonoids prevalently present in fruits and vegetables, could suppress lipopolysaccharide- (LPS-) induced inflammatory responses in macrophages. LPS increased proinflammatory mRNA abundance (MCP 1, IL-1β, and iNOS) but were suppressed by fisetin. The increment of nitric oxide by LPS, an oxidative stress factor, was attenuated by fisetin. In addition, LPS-enhanced phosphorylation of mitogen-activated protein kinase (ERK and JNK) was reduced. Finally, fisetin attenuated the expression or activity of uPA, uPAR, MMP-2, and MMP-9, which are known as associated factors of macrophage recruitment or infiltration. In conclusion, fisetin is a promising therapeutic agent for macrophage-related inflammation diseases, like sepsis and atherosclerosis.

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Inhibition of interleukin-6 signaling attenuates aortitis, left ventricular hypertrophy and arthritis in interleukin-1 receptor antagonist deficient mice

et al. 2020

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The aim of this study was to examine whether inhibition of Interleukin (IL)-6 signaling by MR16-1, an IL-6 receptor antibody, attenuates aortitis, cardiac hypertrophy, and arthritis in IL-1 receptor antagonist deficient (IL-1RA KO) mice. Four weeks old mice were intraperitoneally administered with either MR16-1 or non-immune IgG at dosages that were adjusted over time for 5 weeks. These mice were stratified into 4 groups: MR16-1 treatment groups, KO/MR low group (first 2.0 mg, following 0.5 mg/week, n=14) and KO/MR high group (first 4.0 mg, following 2.0 mg/week, n=19) in IL-1RA KO mice, and IgG treatment groups, KO/IgG group (first 2.0 mg, following 1.0 mg/week, n=22) in IL-1RA KO mice, and wild/IgG group (first 2.0 mg, following 1.0 mg/week, n=17) in wild mice. Aortitis, cardiac hypertrophy and arthropathy were histologically analyzed. Sixty-eight % of the KO/IgG group developed aortitis (53% developed severe aortitis). In contrast, only 21% of the KO/MR high group developed mild aortitis, without severe aortitis (P<0.01, vs KO/IgG group). Infiltration of inflammatory cells, such as neutrophils, T cells, and macrophages, was frequently observed around aortic sinus of the KO/IgG group. Left ventricle and cardiomyocyte hypertrophy were observed in IL-1RA KO mice. Administration of high dosage of MR16-1 significantly suppressed cardiomyocyte hypertrophy. MR16-1 attenuated the incidence and severity of arthritis in IL-1RA KO mice in a dose-dependent manner. In conclusion, blockade of IL-6 signaling may exert a beneficial effect to attenuate severe aortitis, left ventricle hypertrophy, and arthritis

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Exogenous Vasohibin-2 Exacerbates Angiotensin II-Induced Ascending Aortic Dilation in Mice

Cited by 8 publications

References 46 publications

The Protective Effect of Chlorogenic Acid on Vascular Senescence via the Nrf2/HO-1 Pathway

The Protective Effect of Chlorogenic Acid on Vascular Senescence via the Nrf2/HO-1 Pathway

Fisetin Attenuates Lipopolysaccharide-Induced Inflammatory Responses in Macrophage

Inhibition of interleukin-6 signaling attenuates aortitis, left ventricular hypertrophy and arthritis in interleukin-1 receptor antagonist deficient mice

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