Exoglucanase production by Aspergillus niger grown on wheat bran

Chandra, Muniramanna Gari Subhosh; Reddy, B. Rajasekhar

doi:10.1007/s13213-012-0538-0

Cited by 16 publications

(2 citation statements)

References 35 publications

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“…However, the exact optimal inoculum size varies with specific conditions (strains, inoculation strategies, and so on). Subhosh Chandra et al optimized the inoculum size of 2 × 10 6 spores/g for maximum metabolites using WB . With the increase of inoculation amount, the target fungi inhibit the survival of other microorganisms to become the dominant colony in the matrix, thus shortening the delay period and further accelerating the synthesis and secretion of specific enzymes.…”

Section: Resultsmentioning

confidence: 99%

Process Optimization for Production of Ferulic Acid and Pentosans from Wheat Brans by Solid-State Fermentation and Evaluation of Their Antioxidant Activities

Zhang

Zhou

Zhang

et al. 2022

ACS Food Sci. Technol.

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Wheat bran (WB) is largely discarded during processing, resulting in wastage of the bioactive components in it, such as ferulic acid (FA) and pentosans. The aim of this study was to enhance their yield by solid-state fermentation with three filamentous fungi (Aspergillus oryzae, Rhizopus oryzae, and Aspergillus niger). Through single-factor experiments, the optimal fermentation conditions were found to be a strain of A. oryzae, an inoculation amount of A. oryzae of 4 × 10 5 spores/g, a moisture content of 60%, a fermentation temperature of 28 °C, and a fermentation time of 48 h. By 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) methods, the antioxidant activities of fermented WB were found to be significantly improved. Also, there was a good correlation between the total phenolics content (TPC) and antioxidant capacity. Briefly, fermentation not only provides a new avenue to increase the functional potential of WB, but also enhances the utilization of agricultural byproducts.

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Section: Resultsmentioning

confidence: 99%

Process Optimization for Production of Ferulic Acid and Pentosans from Wheat Brans by Solid-State Fermentation and Evaluation of Their Antioxidant Activities

Zhang

Zhou

Zhang

et al. 2022

ACS Food Sci. Technol.

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“…(2) Exoglucanase Activity. Exoglucanase activity as flter paperase (FPase) activity in the hydrolyzate was determined according to the method described by Chandra and Reddy [52] as follows: 1 mL of the hydrolyzate (i.e., the cocktail enzymes) was mixed with 2 mL of 0.1 M citrate bufer pH 4.8 containing 0.05 g of flter paper (Whatman no.1). Te tube was incubated at 50 °C for 1 h. Te liberated TRSs were measured by the DNSA method, as described before, at λ 540 nm .…”

Section: (Hemi) Cellulolytic Enzymes Assaysmentioning

confidence: 99%

A Study on the Valorization of Rice Straw into Different Value‐Added Products and Biofuels

Ali,

Hosny,

Nassar

et al. 2024

International Journal of Chemical Engineering

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This work depicts that rice straw (RS), which is one of the major lignocellulosic wastes all over the world and causing many environmental problems, has considerable amounts of protein, ash, macronutrients, and micronutrients of approximately 11.38%, 16.77%, 2.27 mg/kg, and 771.9 mg/kg, respectively; besides, a C/N ratio of 15.18, a total N, P2O5, and K2O content of 1.85%, and a considerably low concentration of undesirable heavy metals and silica of approximately 77.69 mg/kg and 109 mg/kg are also present, which recommends its applicability as a precursor feedstock for the production of organic fertilizer and animal fodder. The batch solid-state fermentation (SSF) of RS by Trichoderma longibrachiatum DSMZ 16517 produced considerable amount of total reducing sugars (TRS) of approximately 339.2 mg TRS/g RS under the optimum operatic conditions of 20% (w:v) substrate concentration, pH 7, 1% inoculum size, a 9-day incubation period, and 30°C incubation temperature. The readily available and cost-effective agroindustrial waste, sugarcane molasses, proved to enhance the fungal biomass growth and (hemi) cellulolytic enzymes activities. The inoculated RS-SSF batch process with T. longibrachiatum precultured on 10% molasses enhanced the (hemi) cellulolytic enzymatic activities and TRS production rate by approximately 5.82 and 3.8 folds, respectively, relative to that inoculated by T. longibrachiatum precultured in the conventional potato dextrose broth medium. The separate hydrolysis and fermentation processes by different yeast strains Candida tropicalis DSM 70156, C. shehatae ATCC 58779, and Saccharomyces cerevisiae ATCC 64712 revealed an efficient bioethanol yield and productivity that ranged between 0.36 and 0.38 g/g sugars and 0.22 and 0.23 g/L/h, respectively, with concomitant competent fermentation efficiencies that ranged between 48.35% and 51.25%. The proximate analysis of rice straw before and after fungal hydrolysis proved calorific values of approximately 15.8 MJ/kg and 16.05 MJ/kg, respectively, recommending their applicability as primary and secondary solid biofuels. Thus, this study proved the waste prosperity of RS for environmental opulence and sustainability.

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Value Added Products from Agriculture, Paper and Food Waste: A Source of Bioenergy Production

Chandra

Srinivasulu

Yadav

et al. 2021

Clean Energy Production Technologies

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Exoglucanase production by Aspergillus niger grown on wheat bran

Cited by 16 publications

References 35 publications

Process Optimization for Production of Ferulic Acid and Pentosans from Wheat Brans by Solid-State Fermentation and Evaluation of Their Antioxidant Activities

Process Optimization for Production of Ferulic Acid and Pentosans from Wheat Brans by Solid-State Fermentation and Evaluation of Their Antioxidant Activities

A Study on the Valorization of Rice Straw into Different Value‐Added Products and Biofuels

Value Added Products from Agriculture, Paper and Food Waste: A Source of Bioenergy Production

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