Exometabolomic analysis of decidualizing human endometrial stromal and perivascular cells

Harden, Sarah; Zhou, Jie; Diniz-da-Costa, Maria; Lucas, Emma S.; Liang, Cui; Murakami, Keisuke; Fang, Jinling; Chen, Yi‐Ping Phoebe; Brosens, Jan J.; Lee, Yie Hou

doi:10.1101/2020.08.06.221119

Cited by 3 publications

(3 citation statements)

References 70 publications

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“…Siobhan Quenby and Lauren Lacey and the staff of the Biomedical Research Unit at University Hospitals Coventry and Warwickshire NHS Trust for their help with sample collection. This article was submitted following preprint on BioRxiv (doi: https://doi.org/10.1101/2020.08.06.221119 ) ( Harden et al, 2020 ).…”

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confidence: 99%

Exometabolomic Analysis of Decidualizing Human Endometrial Stromal and Perivascular Cells

Harden

Zhou

Gharanei

et al. 2021

Front. Cell Dev. Biol.

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Differentiation of endometrial fibroblasts into specialized decidual cells controls embryo implantation and transforms the cycling endometrium into a semi-permanent, immune-protective matrix that accommodates the placenta throughout pregnancy. This process starts during the midluteal phase of the menstrual cycle with decidual transformation of perivascular cells (PVC) surrounding the terminal spiral arterioles and endometrial stromal cells (EnSC) underlying the luminal epithelium. Decidualization involves extensive cellular reprogramming and acquisition of a secretory phenotype, essential for coordinated placental trophoblast invasion. Secreted metabolites are an emerging class of signaling molecules, collectively known as the exometabolome. Here, we used liquid chromatography-mass spectrometry to characterize and analyze time-resolved changes in metabolite secretion (exometabolome) of primary PVC and EnSC decidualized over 8 days. PVC were isolated using positive selection of the cell surface marker SUSD2. We identified 79 annotated metabolites differentially secreted upon decidualization, including prostaglandin, sphingolipid, and hyaluronic acid metabolites. Secreted metabolites encompassed 21 metabolic pathways, most prominently glycerolipid and pyrimidine metabolism. Although temporal exometabolome changes were comparable between decidualizing PVC and EnSC, 32 metabolites were differentially secreted across the decidualization time-course. Further, targeted metabolomics demonstrated significant differences in secretion of purine pathway metabolites between decidualized PVC and EnSC. Taken together, our findings indicate that the metabolic footprints generated by different decidual subpopulations encode spatiotemporal information that may be important for optimal embryo implantation.

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confidence: 99%

Exometabolomic Analysis of Decidualizing Human Endometrial Stromal and Perivascular Cells

Harden

Zhou

Gharanei

et al. 2021

Front. Cell Dev. Biol.

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“…Metabolomics/metabonomics [ 35 ], exosome metabolomics (exometabolomics) [ 35 ], and multi-OMICS approaches [ 36 , 37 ] represent further innovative strategies for (sub-)typing gynecological patients. Finally, phenomics [ 38 ] aims at systematically and comprehensively measuring and quantitatively assessing the human phenome, which is the array of physical, chemical, biochemical, and biophysical traits expressed and/or generated by an individual during its development, adaptation to genetic influences, and environmental stimuli.…”

Section: Gynecology and Omics Datamentioning

confidence: 99%

Gynecology Meets Big Data in the Disruptive Innovation Medical Era: State-of-Art and Future Prospects

Khamisy‐Farah

Furstenau

Kong

et al. 2021

IJERPH

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Tremendous scientific and technological achievements have been revolutionizing the current medical era, changing the way in which physicians practice their profession and deliver healthcare provisions. This is due to the convergence of various advancements related to digitalization and the use of information and communication technologies (ICTs)—ranging from the internet of things (IoT) and the internet of medical things (IoMT) to the fields of robotics, virtual and augmented reality, and massively parallel and cloud computing. Further progress has been made in the fields of addictive manufacturing and three-dimensional (3D) printing, sophisticated statistical tools such as big data visualization and analytics (BDVA) and artificial intelligence (AI), the use of mobile and smartphone applications (apps), remote monitoring and wearable sensors, and e-learning, among others. Within this new conceptual framework, big data represents a massive set of data characterized by different properties and features. These can be categorized both from a quantitative and qualitative standpoint, and include data generated from wet-lab and microarrays (molecular big data), databases and registries (clinical/computational big data), imaging techniques (such as radiomics, imaging big data) and web searches (the so-called infodemiology, digital big data). The present review aims to show how big and smart data can revolutionize gynecology by shedding light on female reproductive health, both in terms of physiology and pathophysiology. More specifically, they appear to have potential uses in the field of gynecology to increase its accuracy and precision, stratify patients, provide opportunities for personalized treatment options rather than delivering a package of “one-size-fits-it-all” healthcare management provisions, and enhance its effectiveness at each stage (health promotion, prevention, diagnosis, prognosis, and therapeutics).

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“…These secreted metabolites encompassed 21 metabolic pathways: the most prominent ones are glycerolipid and pyrimidine metabolites. 55 However, this is just the first step to highlight the potential of secreted metabolites acting as autocrine or paracrine signaling molecules as the decidualization process unfolds.…”

Section: Metabolites Regulating Decidualizationmentioning

confidence: 99%

Decidualization and Related Pregnancy Complications

Tong

Yang

et al. 2021

Maternal-Fetal Medicine

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Decidualization is the differentiation of endometrial stromal cells into secretory decidual stromal cells. Human decidualization involves some amount of signaling molecules and pathways as well as genetic reprogramming, which is driven by the postovulatory rise in progesterone levels and local cyclic adenosine monophosphate production. Decidualization extends from the primary decidual zone to the secondary decidual zone, and then exits through apoptosis. Evidences support that decidual fibroblasts function as the pool of decidual stromal cells during pregnancy. Decidualization undergoes an acute inflammatory phase, an anti-inflammatory secretory phase to the final recession phase. The decidualization of the inner layer of endometrium, termed decidua, is the most critical determinant of pregnancy success, which can promote placenta formation, modulate immune tolerance, foster resistance to oxidative stress, sense embryo quality, and control labor. Failure to adequate decidualization in terms of hormones, biochemistry, and immunology leads to adverse pregnancy outcomes, including diseases such as preeclampsia, miscarriage, premature labor, repeated implantation failures, and some age-related decline in reproductive capacity. The development of animal models and in vitro culture systems combined with emerging technologies provides a powerful system to explore the mechanism of decidualization. However, decidualization is a dynamic, multi-step process, and translating of current research progress into disease predictions and interventions for pregnancy complications remains to be achieved. The study of periodic regeneration and spontaneous decidualization of the endometrium will be beneficial to the diagnosis and treatment of pregnancy diseases.

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Exometabolomic analysis of decidualizing human endometrial stromal and perivascular cells

Cited by 3 publications

References 70 publications

Exometabolomic Analysis of Decidualizing Human Endometrial Stromal and Perivascular Cells

Exometabolomic Analysis of Decidualizing Human Endometrial Stromal and Perivascular Cells

Gynecology Meets Big Data in the Disruptive Innovation Medical Era: State-of-Art and Future Prospects

Decidualization and Related Pregnancy Complications

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