Exon III Splicing of Fibroblast Growth Factor Receptor 1 Is Modulated by Growth Factors and Cyclin D1

Chen, Guowei; Wang, Jian; Liu, Zhanbing; Kornmann, Marko

doi:10.1097/mpa.0b013e31816618a4

Cited by 11 publications

(7 citation statements)

References 28 publications

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“…The establishment of the FGFR1-IIIb and -IIIc PANC-1 clones over-expressing the full-length cDNA of human FGFR1-IIIb or -IIIc expressed in a modified pSVK3 vector under the control of the simian virus 40 early promotor in a stable manner was described earlier (Liu et al, 2007b;Chen et al, 2008).…”

Section: Establishment Of Cell Clones Over-expressing Fgfr1-iii Variantsmentioning

confidence: 99%

Inhibition of endogenous SPARC enhances pancreatic cancer cell growth: modulation by FGFR1-III isoform expression

et al. 2009

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BACKGROUND: Secreted protein acidic and rich in cysteine (SPARC) is a multi-faceted protein-modulating cell -cell and cell -matrix interactions. In cancer, SPARC can be not only associated with a highly aggressive phenotype, but also acts as a tumour suppressor. The aim of this study was to characterise the function of SPARC and its modulation by fibroblast growth factor receptor (FGFR) 1 isoforms in pancreatic ductal adenocarcinoma (PDAC). METHODS AND RESULTS: Exogenous SPARC inhibited growth, movement, and migration. ShRNA inhibition of endogenous SPARC in ASPC-1 and PANC-1 cells resulted in increased anchorage-dependent and -independent growth, transwell migration, and xenograft growth as well as increased mitogenic efficacy of fibroblast growth factor (FGF) 1 and FGF2. Endogenous SPARC expression in PANC-1 cells was increased in FGFR1-IIIb over-expressing cells, but decreased in FGFR1-IIIc over-expressing cells. The up-regulation of endogenous SPARC was abrogated by the p38-mitogen-activated protein kinase inhibitor SB203580. SPARC was detectable in conditioned medium of pancreatic stellate cells (PSCs), but not PDAC cells. Conditioned medium of PDAC cells reduced endogenous SPARC expression of PSCs. CONCLUSION: Endogenous SPARC inhibits the malignant phenotype of PDAC cells and may, therefore, act as a tumour suppressor in PDAC. Endogenous SPARC expression can be modulated by FGFR1-III isoform expression. In addition, PDAC cells may inhibit endogenous SPARC expression in surrounding PSCs by paracrine actions.

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Section: Establishment Of Cell Clones Over-expressing Fgfr1-iii Variantsmentioning

confidence: 99%

Inhibition of endogenous SPARC enhances pancreatic cancer cell growth: modulation by FGFR1-III isoform expression

et al. 2009

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“…Furthermore, more recently, it has been reported that the inhibition of cyclin D1 using constitutively active cyclin D1 antisense cDNA expression vector resulted in the suppression of FGFR 1-IIIc mRNA expression, one of the alternative splice variants of FGFR 1, in human pancreatic cancer PANC-1 cells (Chen et al, 2008). In the present study, we also found that the expression of FGFR 1 is up-regulated in E2F-1-overexpressing LoVo cells, and down-regulated in pRB-overexpressing HT1080 cells.…”

Section: Discussionmentioning

confidence: 99%

Transcriptional regulation of human fibroblast growth factor receptor 1 by E2F-1

Kanai

Tashiro

Maruki

et al. 2009

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“…The summarized results are shown for clone P19-13 in Figure 1 (BYD) and were shown for clone P19-22 previously. 23 …”

Section: Establishment Of Tet-inducible Cyclin D1 Antisense Expressinmentioning

confidence: 99%

Inhibition of Pancreatic Cancer Cell Growth In Vivo Using a Tetracycline-Inducible Cyclin D1 Antisense Expression System

et al. 2013

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Exon III Splicing of Fibroblast Growth Factor Receptor 1 Is Modulated by Growth Factors and Cyclin D1

Abstract: This study demonstrated for the first time that FGFR1-IIIb and FGFR1-IIIc are coexpressed and that the FGFR1-III isoformsare differentially regulated by growth factors and cyclin D1.

Cited by 11 publications

References 28 publications

Inhibition of endogenous SPARC enhances pancreatic cancer cell growth: modulation by FGFR1-III isoform expression

Inhibition of endogenous SPARC enhances pancreatic cancer cell growth: modulation by FGFR1-III isoform expression

Transcriptional regulation of human fibroblast growth factor receptor 1 by E2F-1

Inhibition of Pancreatic Cancer Cell Growth In Vivo Using a Tetracycline-Inducible Cyclin D1 Antisense Expression System

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