Exonuclease III-aided recycling amplification of proximity ligation assay using thymine-melamine-thymine triplex structure for ultrasensitive fluorometric determination of melamine

Leng, Xueqi; Tu, Yuqin; Wu, Yiming; Wang, Yu; Liu, Su; Pei, Qianqian; Cui, Xuejun

doi:10.1016/j.foodcont.2018.05.023

Cited by 7 publications

(2 citation statements)

References 36 publications

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“…With the ideal sensitivity and selectivity, the applicability of this method to real milk samples was examined. Because the melamine-milk sample was not obtained in hand, we took the spiked milk to evaluate the reliability as other papers [ 15 , 16 , 18 , 26 ]. Standard addition method was used on three standard milk samples and the final melamine concentrations were obtained as 20, 80, 150 μM.…”

Section: Resultsmentioning

confidence: 99%

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Simple and Label-Free Fluorescent Detection of Melamine Based on Melamine–Thymine Recognition

Yang

Wang

et al. 2018

Sensors

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In the past few years, melamine has been illegally added into dairy products to increase the apparent crude protein levels. If humans or animals drink the milk adulteration of melamine, it can form insoluble melamine–cyanurate crystals in their kidneys which causes kidney damage or even death. In the present work, we constructed a simple and label-free fluorescent method for melamine detection based on melamine-thymine recognition. SYBR Green I was utilized as a reporter for this method as it did not require any modification or expensive equipment. In the absence of melamine, polythymine DNA was digested by Exo I, which caused a decrease in the fluorescence signal. In the presence of melamine, the polythymine DNA was able to fold into a double chain structure, however this was done with the help of T-melamine-T mismatches to prevent degradation. Then, the SYBR Green I combined with the double-stranded DNA to result in an intense fluorescence signal. The limit of detection in this method was 1.58 μM, which satisfied the FDA standards. This method also had a good linear relationship within the range of 10–200 μM. In addition, this new method has a good selectivity to distinguish melamine from the component of milk. As a result, we developed a simple and highly selectivity method for melamine detection.

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Section: Resultsmentioning

confidence: 99%

“…Recently, some papers have reported that melamine can form T-melamine-T mismatches in oligonucleotides by triple hydrogen bonding with thymine base [ 23 , 24 , 25 , 26 ]. Furthermore, SYBR Green I can exhibit a dramatic enhancement upon binding to double-stranded DNA [ 27 , 28 ].…”

Section: Introductionmentioning

confidence: 99%