Exophiala dermatitis and exacerbation of chronic obstructive pulmonary disease

Gu, Jinjin; Xu, Jiayi; Su, Qianya; Chen, Y

doi:10.1093/qjmed/hcz190

Cited by 3 publications

(4 citation statements)

References 5 publications

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“…Both chromoblastomycosis (CBM) and phaeohyphomycosis (FEO), when affecting the skin and subcutaneous tissues, can be persistent and challenging to treat, often requiring prolonged therapies and, in some cases, surgical interventions [1,8,[22][23][24]. In immunocompromised patients, such as those with HIV/AIDS, uncontrolled diabetes, or transplant recipients, agents causing phaeohyphomycosis can disseminate to internal organs and the central nervous system, posing a severe life-threatening risk [1, 25,26].…”

Section: Discussionmentioning

confidence: 99%

“…Besides the agents of chromoblastomycosis, it is possible to identify two species responsible for phaeohyphomycosis. The first is Exophiala dermatitidis, a causative agent of cutaneous, subcutaneous, and invasive infections [26,39,40], which, in severe cases, can affect the central nervous system [41]. The second identifiable species is Cladophialophora bantiana, a causative agent of PHM with high lethality due to invasive infections, mainly affecting the central nervous system of immunocompromised and post-transplant patients [25,[40][41][42].…”

Section: Discussionmentioning

confidence: 99%

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Identification of Chromoblastomycosis and Phaeohyphomycosis Agents through ITS-RFLP

Sousa,

De Oliveira,

De Souza

et al. 2024

JoF

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Chromoblastomycosis (CBM) and phaeohyphomycosis (FEO) are infections caused by melanized filamentous fungal agents, primarily found in tropical and subtropical regions. Both infections pose significant challenges for the correct identification of the causative agent due to their morphological similarity, making conventional methods of morphological analysis highly subjective. Therefore, molecular techniques are necessary for the precise determination of these species. In this regard, this study aimed to contribute to a new methodology based on PCR-RFLP for the identification of agents causing CBM and FEO. Sequences from the Internal Transcribed Spacer (ITS) region were used to identify potential restriction enzyme sites in silico, followed by in vitro validation using the selected restriction enzymes. The obtained results were compared with species identification through morphological analyses and sequencing. The results demonstrated that the PCR-RFLP applied in this study accurately identified two major agents of chromoblastomycosis, Fonsecaea pedrosoi and Fonsecaea monophora, as well as Cladophialophora bantiana and Exophiala dermatitidis, both causative agents of phaeohyphomycosis. In this context, the proposed assay can complement current methods for identifying these species, aiding in diagnosis, and contributing to the proper management of these infections.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Identification of Chromoblastomycosis and Phaeohyphomycosis Agents through ITS-RFLP

Sousa,

De Oliveira,

De Souza

et al. 2024

JoF

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“…The failure to determine these agents directly impacts the management of these infections, as depending on the species, the infection can lead to severe systemic commitment [14,31,32] or be resistant to certain antifungal therapy [33][34][35]. Besides the clinical context, the identification of the agent is crucial for the epidemiological monitoring of these infections, providing data for control and prevention strategies [7].…”

Section: Discussionmentioning

confidence: 99%

Development of PCR-Multiplex Assays for Identification of the Herpotrichiellaceae Family and Agents Causing Chromoblastomycosis

Sousa,

De Oliveira,

De Souza

et al. 2024

Preprint

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The Herpotrichiellaceae family is an important group of dematiaceous filamentous fungi, associated with a variety of pathogenic fungal species causing Chromoblastomycosis (CBM) and Phaeohyphomycosis (PHM), both with polymorphic clinical manifestations and worldwide incidence. Currently, the identification of this family and determination of the causative agent is challenging due to the subjectivity of morphological identification methods, necessitating the use of molecular techniques to complement diagnosis. In this context, genetic sequencing of the Internal Transcribed Spacer (ITS) has become the norm due to a lack of alternative molecular tools for identifying these agents. Therefore, this study aimed to develop PCR-Multiplex methodologies to address this gap. Sequences from the ITS and Large subunit (LSU) of ribosomal DNA were used, and after manual curation and in vitro analyses, primers were synthesized for the identification of the aforementioned targets. The primers were optimized and validated in vitro, resulting in two PCR-Multiplex methodologies: one for identifying the Herpotrichiellaceae family and the bantiana clade, and another for determining the species Fonsecaea pedrosoi and Fonsecaea monophora. Ultimately, the assays developed in this study aim to complement other identification approaches for these agents, reducing the need for sequencing, improving the management of these infections, and enhancing the accuracy of epidemiological information.

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Section: Discussionmentioning

confidence: 99%

Identification of Chromoblastomycosis and Phaeohyphomycosis Agents Through ITS-RFLP

Sousa,

De Oliveira,

De Souza

et al. 2023

Preprint

View full text Add to dashboard Cite

Chromoblastomycosis (CBM) and phaeohyphomycosis (FEO) are infections caused by melanized filamentous fungal agents, primarily found in tropical and subtropical regions. Both infections pose significant challenges for the correct identification of the causative agent due to their morphological similarity, making conventional methods of morphological analysis highly subjective. Therefore, molecular techniques are necessary for the precise determination of these species. In this regard, this study aimed to contribute to a new methodology based on PCR-RFLP for the identification of agents causing CBM and FEO. Sequences from the ITS region were used to identify potential restriction enzyme sites in silico, followed by in vitro validation using the selected restriction enzymes. The obtained results were compared with species identification through morphological analyses and sequencing. The results demonstrated that the PCR-RFLP applied in this study accurately identified two major agents of chromoblastomycosis, Fonsecaea pedrosoi and Fonsecaea monophora, as well as Cladophialophora bantiana and Exophiala dermatitidis, both causative agents of phaeohyphomycosis. In this context, the proposed assay can complement current methods for identifying these species, aiding in diagnosis, and contributing to the proper management of these infections.

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Exophiala dermatitis and exacerbation of chronic obstructive pulmonary disease

Cited by 3 publications

References 5 publications

Identification of Chromoblastomycosis and Phaeohyphomycosis Agents through ITS-RFLP

Identification of Chromoblastomycosis and Phaeohyphomycosis Agents through ITS-RFLP

Development of PCR-Multiplex Assays for Identification of the Herpotrichiellaceae Family and Agents Causing Chromoblastomycosis

Identification of Chromoblastomycosis and Phaeohyphomycosis Agents Through ITS-RFLP

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