Exopolysaccharide Biosynthesis Enables Mature Biofilm Formation on Abiotic Surfaces by Herbaspirillum seropedicae

Balsanelli, Eduardo; Baura, Valter A.; Pedrosa, Fábio de Oliveira; Souza, Emanuel Maltempi de; Monteiro, Rose A.

doi:10.1371/journal.pone.0110392

Cited by 63 publications

(35 citation statements)

References 70 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In contrast, exopolysaccharide production involved in biofilm formation and attachment to inert surfaces was not important for epiphytic or inner colonization of maize roots by Herbaspirillum (9).…”

Section: Into the Roots: Ways Endophytes Get Inmentioning

confidence: 72%

Roots Shaping Their Microbiome: Global Hotspots for Microbial Activity

Reinhold‐Hurek

Bünger

Burbano

et al. 2015

Annu. Rev. Phytopathol.

618

379

View full text Add to dashboard Cite

Land plants interact with microbes primarily at roots. Despite the importance of root microbial communities for health and nutrient uptake, the current understanding of the complex plant-microbe interactions in the rhizosphere is still in its infancy. Roots provide different microhabitats at the soil-root interface: rhizosphere soil, rhizoplane, and endorhizosphere. We discuss technical aspects of their differentiation that are relevant for the functional analysis of their different microbiomes, and we assess PCR (polymerase chain reaction)-based methods to analyze plant-associated bacterial communities. Development of novel primers will allow a less biased and more quantitative view of these global hotspots of microbial activity. Based on comparison of microbiome data for the different root-soil compartments and on knowledge of bacterial functions, a three-step enrichment model for shifts in community structure from bulk soil toward roots is presented. To unravel how plants shape their microbiome, a major research field is likely to be the coupling of reductionist and molecular ecological approaches, particularly for specific plant genotypes and mutants, to clarify causal relationships in complex root communities.

show abstract

Section: Into the Roots: Ways Endophytes Get Inmentioning

confidence: 72%

Roots Shaping Their Microbiome: Global Hotspots for Microbial Activity

Reinhold‐Hurek

Bünger

Burbano

et al. 2015

Annu. Rev. Phytopathol.

618

379

View full text Add to dashboard Cite

show abstract

“…Three days after inoculation, epiphytic cells upregulated the genes Hsero_0588 , Hsero_1168 and Hsero_1219 (102‐fold, 11‐fold and fivefold, respectively), coding for ExbD‐like polysaccharides export systems, and Hsero_2761 (sevenfold) encoding a Wza‐like outer membrane polysaccharide export protein, suggesting increased production of polysaccharides. However, the eps gene cluster containing genes for exopolysaccharide biosynthesis was not expressed in any of the conditions, a result in agreement with our previous observation that eps genes are not required for colonization and are in fact downregulated during the plant interaction (Balsanelli et al ., ). The results suggest that unidentified exopolysaccharide biosynthesis genes might be activated during colonization, or potentially the transporters are responsible for export of other molecules, e.g.…”

Section: Resultsmentioning

confidence: 99%

Molecular adaptations of Herbaspirillum seropedicae during colonization of the maize rhizosphere

Balsanelli

Tadra-Sfeir

Faoro

et al. 2015

Environmental Microbiology

Self Cite

View full text Add to dashboard Cite

Molecular mechanisms of plant recognition and colonization by diazotrophic bacteria are barely understood. Herbaspirillum seropedicae is a Betaproteobacterium capable of colonizing epiphytically and endophytically commercial grasses, to promote plant growth. In this study, we utilized RNA-seq to compare the transcriptional profiles of planktonic and maize root-attached H. seropedicae SmR1 recovered 1 and 3 days after inoculation. The results indicated that nitrogen metabolism was strongly activated in the rhizosphere and polyhydroxybutyrate storage was mobilized in order to assist the survival of H. seropedicae during the early stages of colonization. Epiphytic cells showed altered transcription levels of several genes associated with polysaccharide biosynthesis, peptidoglycan turnover and outer membrane protein biosynthesis, suggesting reorganization of cell wall envelope components. Specific methyl-accepting chemotaxis proteins and two-component systems were differentially expressed between populations over time, suggesting deployment of an extensive bacterial sensory system for adaptation to the plant environment. An insertion mutation inactivating a methyl-accepting chemosensor induced in planktonic bacteria, decreased chemotaxis towards the plant and attachment to roots. In summary, analysis of mutant strains combined with transcript profiling revealed several molecular adaptations that enable H. seropedicae to sense the plant environment, attach to the root surface and survive during the early stages of maize colonization.

show abstract

“…L. biflexa can persist over long periods of time in distilled water by forming biofilms, and aggregation has been suggested to allow environmental survival and host colonization [74, 100]. Transport proteins that excrete exopolysaccharides, signaling molecules, and adhesion proteins also promote biofilm formation for persistence [101–103]. …”

Section: Discussionmentioning

confidence: 99%

Comparative analyses of transport proteins encoded within the genomes of Leptospira species

Buyuktimkin

Saier

2016

Microbial Pathogenesis

View full text Add to dashboard Cite

Select species of the bacterial genus Leptospira are causative agents of leptospirosis, an emerging global zoonosis affecting nearly one million people worldwide annually. We examined two Leptospira pathogens, L. interrogans serovar Lai str. 56601 and L. borgpetersenii serovar Hardjo-bovis str. L550, as well as the free-living leptospiral saprophyte, L. biflexa serovar Patoc str. ‘Patoc 1 (Ames)’. The transport proteins of these leptospires were identified and compared using bioinformatics to gain an appreciation for which proteins may be related to pathogenesis and saprophytism. L. biflexa possesses a disproportionately high number of secondary carriers for metabolite uptake and environmental adaptability as well as an increased number of inorganic cation transporters providing ionic homeostasis and effective osmoregulation in a rapidly changing environment. L. interrogans and L. borgpetersenii possess far fewer transporters, but those that they all have are remarkably similar, with near-equivalent representation in most transporter families. These two Leptospira pathogens also possess intact sphingomyelinases, holins, and virulence-related outer membrane porins. These virulence-related factors, in conjunction with decreased transporter substrate versatility, indicate that pathogenicity arose in Leptospira correlating to progressively narrowing ecological niches and the emergence of a limited set of proteins responsible for host invasion. The variability of host tropism and mortality rates by infectious leptospires suggests that small differences in individual sets of proteins play important physiological and pathological roles.

show abstract

Exopolysaccharide Biosynthesis Enables Mature Biofilm Formation on Abiotic Surfaces by Herbaspirillum seropedicae

Cited by 63 publications

References 70 publications

Roots Shaping Their Microbiome: Global Hotspots for Microbial Activity

Roots Shaping Their Microbiome: Global Hotspots for Microbial Activity

Molecular adaptations of Herbaspirillum seropedicae during colonization of the maize rhizosphere

Comparative analyses of transport proteins encoded within the genomes of Leptospira species

Contact Info

Product

Resources

About