Exopolysaccharide from the mice ovarian bacterium Bacillus velezensis OM03 triggers caspase-3-dependent apoptosis in ovarian cancer cells

Abstract

Exopolysaccharides (EPS) were isolated from mice ovarian microflora Bacillus velezensis OM03 to investigate their chemical properties and cytotoxic potential against human ovarian carcinoma cell lines PA-1 and SKOV-3. The structural analysis of EPS from OM03 using Fourier Transform Infra-Red spectroscopy, X-ray diffraction, Scanning Electron Microscopy, and Gas chromatography-Mass revealed that it is a novel heteropolysaccharide made of glucose and mannose units connected with α-1,4 and β-1,4 bonds. Attractively, the EPS inhibited the proliferation of SKOV-3 and PA-1 cancer cells in a concentration-dependent manner, with IC 50 values of 620 and 238 µg/ml, respectively, according to 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide tests. Moreover, EPS-OM03 caused DNA fragmentation in PA-1 cell lines and boosted the expression of active caspase-3, both of which triggered apoptosis. Furthermore, the experiment with the chick embryo chorioallantoic membrane demonstrated that treatment with EPS-OM03 exhibits an in vivo antiangiogenic effect with an IC 50 of 146 µg/ml. In conclusion, our work offers scientific support for the development and use of pharmaceuticals derived from the microbiome, and it suggests that the EPS from B. velezensis OM03 may be a lead molecule in the treatment of ovarian cancer.

Exopolysaccharide from Rhodococcus pyridinivorans ZZ47 Strain: Evaluation of Biological Activity and Toxicity

“…In parallel with our results, an EPS isolated from Bacillus velezensis OM03 has inhibited nearly 95% vascularization at 400 µg/ml concentration by chick CAM. In addition to this, this EPS has significant cytotoxic effect on PA-1 (human ovarian teratocarcinoma) and SKOV-3 (human ovarian cancer) cells without damage normal human ovarian surface epithelial cell line T1074, and has triggered apoptosis of PA-1 cells (Chirakkara & Abraham, 2023). In another study, EPS from Lactobacillus acidophilus with antioxidant properties has inhibited tumor angiogenesis related genes expressions and upregulated anti-angiogenic genes in colon cancer cell lines (Deepak et al, 2016).…”