Exopolysaccharide Produced by Probiotic Strain Lactobacillus paraplantarum BGCG11 Reduces Inflammatory Hyperalgesia in Rats

Dinić, Miroslav; Pecikoza, Uroš; Djokić, Jelena; Stepanović-Petrović, Radica; Milenković, Marina; Stevanović, Magdalena; Filipović, Nenad; Begović, Jelena; Golić, Nataša; Lukić, Jovanka

doi:10.3389/fphar.2018.00001

Cited by 434 publications

(346 citation statements)

References 49 publications

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“…Similarly to CF tissue, pHBE CF cells show increased levels of mesenchymal markers, although not accompanied by decreased levels of epithelial markers. Consistently, TEER values (an indicator of integrity of HBE cell cultures 43 ) were significantly lower in CF vs non-CF pHBE cells (Fig. 2C ), indicative of a leaky epithelium and suggesting impaired organization of cell-cell junctions in CF.…”

Section: Resultssupporting

confidence: 55%

Mutant CFTR Drives TWIST1 mediated epithelial–mesenchymal transition

et al. 2020

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Cystic fibrosis (CF) is a monogenetic disease resulting from mutations in the Cystic Fibrosis Transmembrane conductance Regulator (CFTR) gene encoding an anion channel. Recent evidence indicates that CFTR plays a role in other cellular processes, namely in development, cellular differentiation and wound healing. Accordingly, CFTR has been proposed to function as a tumour suppressor in a wide range of cancers. Along these lines, CF was recently suggested to be associated with epithelial–mesenchymal transition (EMT), a latent developmental process, which can be re-activated in fibrosis and cancer. However, it is unknown whether EMT is indeed active in CF and if EMT is triggered by dysfunctional CFTR itself or a consequence of secondary complications of CF. In this study, we investigated the occurrence of EMT in airways native tissue, primary cells and cell lines expressing mutant CFTR through the expression of epithelial and mesenchymal markers as well as EMT-associated transcription factors. Transepithelial electrical resistance, proliferation and regeneration rates, and cell resistance to TGF-β1induced EMT were also measured. CF tissues/cells expressing mutant CFTR displayed several signs of active EMT, namely: destructured epithelial proteins, defective cell junctions, increased levels of mesenchymal markers and EMT-associated transcription factors, hyper-proliferation and impaired wound healing. Importantly, we found evidence that the mutant CFTR triggered EMT was mediated by EMT-associated transcription factor TWIST1. Further, our data show that CF cells are over-sensitive to EMT but the CF EMT phenotype can be reversed by CFTR modulator drugs. Altogether, these results identify for the first time that EMT is intrinsically triggered by the absence of functional CFTR through a TWIST1 dependent mechanism and indicate that CFTR plays a direct role in EMT protection. This mechanistic link is a plausible explanation for the high incidence of fibrosis and cancer in CF, as well as for the role of CFTR as tumour suppressor protein.

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Section: Resultssupporting

confidence: 55%

Mutant CFTR Drives TWIST1 mediated epithelial–mesenchymal transition

et al. 2020

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“…Previous studies have suggested that the over-production of reactive oxygen species (ROS) may have complex roles in promoting the development of age-related diseases [7]. In addition, the cellular elevation of ROS has been associated with the onset and progression of ageing [8]. Meanwhile, it is widely accepted that ROS not only cause oxidative damage, but are also involved in normal cellular function as signalling molecules, through the oxidation of functional thiols on target proteins [9,10].…”

Section: Introductionmentioning

confidence: 99%

Redox signalling and ageing: insights from Drosophila

Lennicke

Cochemé

2020

Biochemical Society Transactions

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Ageing and age-related diseases are major challenges for the social, economic and healthcare systems of our society. Amongst many theories, reactive oxygen species (ROS) have been implicated as a driver of the ageing process. As by-products of aerobic metabolism, ROS are able to randomly oxidise macromolecules, causing intracellular damage that accumulates over time and ultimately leads to dysfunction and cell death. However, the genetic overexpression of enzymes involved in the detoxification of ROS or treatment with antioxidants did not generally extend lifespan, prompting a re-evaluation of the causal role for ROS in ageing. More recently, ROS have emerged as key players in normal cellular signalling by oxidising redox-sensitive cysteine residues within proteins. Therefore, while high levels of ROS may be harmful and induce oxidative stress, low levels of ROS may actually be beneficial as mediators of redox signalling. In this context, enhancing ROS production in model organisms can extend lifespan, with biological effects dependent on the site, levels, and specific species of ROS. In this review, we examine the role of ROS in ageing, with a particular focus on the importance of the fruit fly Drosophila as a powerful model system to study redox processes in vivo.

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“…C. majus is a plant of great interest for its wide use in folk medicine and also in official phytotherapy. This species is known to synthesize a broad range of secondary metabolites which provide its therapeutic properties [ 3 ]. The most common group of these secondary metabolites are isoquinoline alkaloids (1–2% in the herb and 2–4% in the roots), including chelidonine, berberine, sanguinarine, coptisine, chelerythrine and protopine [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

Agro-Morphological, Microanatomical and Molecular Cytogenetic Characterization of the Medicinal Plant Chelidonium majus L.

Samatadze

Yurkevich

Хазиева

et al. 2020

Plants

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Chelidonium majus L. is a medicinal plant well-known as a valuable source of isoquinoline alkaloids, which has a variety of pharmacological properties including anti-viral and anti-bacterial effects. However, considerable intraspecific bio-morphological variability in C. majus complicates raw material identification and verification. For the first time, we have brought into cultivation five populations of C. majus subsp. majus originated from different regions, and performed their agro-morphological, microanatomical and molecular cytogenetic characterization. All examined populations produced high seed (18.6–19.9 kg/ha) and raw material (0.84–1.08 t/ha) yields; total alkaloid contents were within 0.30–0.38%. Nevertheless, significant differences in plant morphology and yield-contributing traits were observed. The performed microanatomical analysis of leaves and flowers in double- and normal-flowered plants revealed micro-diagnostic features (including tissue topography, types of stomata, laticifers, structure of leaf mesophyll, hairs, sepals and petals) important for identification of C. majus raw materials. The analysis of chromosome morphology, DAPI-banding patterns, FISH mapping of 45S and 5S rDNA and also chromosome behavior in meiosis allowed us to identify for the first time all chromosomes in karyotypes and confirm relative genotype stability of the studied plants. Our findings indicate that the examined C. majus populations can be used in further breeding programs.

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Exopolysaccharide Produced by Probiotic Strain Lactobacillus paraplantarum BGCG11 Reduces Inflammatory Hyperalgesia in Rats

Cited by 434 publications

References 49 publications

Mutant CFTR Drives TWIST1 mediated epithelial–mesenchymal transition

Mutant CFTR Drives TWIST1 mediated epithelial–mesenchymal transition

Redox signalling and ageing: insights from Drosophila

Agro-Morphological, Microanatomical and Molecular Cytogenetic Characterization of the Medicinal Plant Chelidonium majus L.

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