Exopolysaccharides from Enterococcus faecium and Streptococcus thermophilus: Bioactivities, gut microbiome effects, and fermented milk rheology

Tarique, Mohammed; Ali, Abdelmoneim H.; Kizhakkayil, Jaleel; Liu, Shao-Quan; Oz, Fatih; Dertli, Enes; Kamal-Eldin, Afaf; Ayyash, Mutamed

doi:10.1016/j.fochx.2023.101073

Cited by 5 publications

(3 citation statements)

References 46 publications

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“…All the biofunctional activities were carried out according to methods detailed in [23,24] unless otherwise mentioned.…”

Section: Biofunctional Activities Of Upsmentioning

confidence: 99%

“…The ACE inhibitory activity of purified UPS was evaluated according to Tarique et al [23]. Absorbance measurements at 228 nm were used to calculate the percentage rate of inhibition using Eq.…”

Section: Angiotensin-converting Enzyme (Ace) Inhibitionmentioning

confidence: 99%

“…The MIC of UPS against four common food pathogens, including Escherichia coli 0157:H7 1934, Salmonella Typhimurium 02-8423, Staphylococcus aureus ATCC 25923, and Listeria monocytogenes DSM 20649) was determined through a specific method involving dilution and incubation. Absorbance measurements at 600 nm were used to calculate the percentage rate of inhibition [23].…”

Section: Minimum Inhibitory Concentrations (Mic)mentioning

confidence: 99%

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Date pomace polysaccharide: ultrasonic-assisted deep eutectic solvent extraction, physicochemical properties, biological activities, gut microbiota modulation, and rheological properties

Bamigbade,

Subhash,

Tarique

et al. 2024

Chem. Biol. Technol. Agric.

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Background This study utilized ultrasonication-assisted green extraction techniques to explore the physicochemical, rheological, biological, and prebiotic properties, alongside gut modulation abilities of novel polysaccharides extracted from date pomace. The extraction aimed at enhancing the utilization of date pomace, a by-product of date fruit processing, by investigating its potential as a functional food ingredient. The research focused on optimizing the extraction process, understanding the complex structure of the polysaccharides, and assessing their various health-related functionalities. Results The ultrasonically extracted polysaccharides (UPS) were identified as a mixture of significant bioactive compounds including galacturonic acid, galactose, glucose, arabinose, and fructose, showcasing a high molecular weight of 537.7 kDa. The study found that UPS exhibited outstanding antioxidant activities, with scavenging abilities ranging from 59 to 82% at a concentration of 1000 mg/L. Additionally, UPS demonstrated potent inhibitory effects on α-amylase (83%), α-glucosidase (81%), and ACE-inhibition (45%), alongside strong antiproliferative activities against Caco-2 and MCF-7 cancer cell lines and broad-spectrum antimicrobial properties. Remarkably, UPS also enhanced the abundance of beneficial gut microbiota, including Actinobacteria, Firmicutes, and Proteobacteria, during in vitro fermentations and positively modulated gut metabolic pathways, promoting the production of major short-chain fatty acids. UPS had higher abundance in pathways related to cofactors, vitamins, electron carriers, and prosthetic groups biosynthesis compared to blank. Conclusions The findings affirm the potential of UPS extracted from date pomace as an innovative and promising functional food ingredient. Its high molecular weight, complex sugar composition, significant antioxidant, antimicrobial, antiproliferative activities, and prebiotic properties make it a valuable resource for promoting health and managing diseases. This study paves the way for further research on the bioavailability and physiological effects of UPS in vivo, highlighting the importance of sustainable utilization of agricultural by-products in developing functional foods that support human health. Graphical Abstract

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“…All the biofunctional activities were carried out according to methods detailed in [23,24] unless otherwise mentioned.…”

Section: Biofunctional Activities Of Upsmentioning

confidence: 99%

Section: Angiotensin-converting Enzyme (Ace) Inhibitionmentioning

confidence: 99%

Section: Minimum Inhibitory Concentrations (Mic)mentioning

confidence: 99%

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