Exopolysaccharides from  Lactobacillus delbrueckii  OLL1073R-1 modulate innate antiviral immune response in porcine intestinal epithelial cells

Kanmani, Paulraj; Albarracín, Leonardo; Kobayashi, Hiroe; Iida, Hikaru; Komatsu, Ryoya; Kober, A. K. M. Humayun; Ikeda-Ohtsubo, Wakako; Suda, Yoshihito; Aso, Hisashi; Makino, Seiya; Kano, Hiroshi; Saito, Tadao; Villena, Julio; Kitazawa, Haruki

doi:10.1016/j.molimm.2017.07.009

Cited by 84 publications

(63 citation statements)

References 33 publications

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“…Interestingly, although EPS improved IFN-β, MxA, and RNaseL expression in PIE cells, this effect was not similar with purified APS or NPS from the OLL1073R-1 strain. In fact, our results indicated that the complete EPS molecule was necessary for obtaining the highest immunomodulatory/antiviral activity in PIE cells ( 47 ). On the other hand, the results presented here indicate that the beneficial effect induced by the TUA4408L strain can be reproduced completely by APS treatment, indicating that this EPS fraction is involved in the modulation of antiviral immunity.…”

Section: Discussionmentioning

confidence: 89%

“…In a recent study, we evaluated the immunomodulatory activities of L. delbrueckii subsp. bulgaricus OLL1037R-1 and demonstrated that its EPS was able to induce a significant increase in the expression of IFN-α, IFN-β, MxA, and RNaseL in PIE cells after the stimulation of TLR3, in a TLR2 dependent manner ( 47 ). Interestingly, although EPS improved IFN-β, MxA, and RNaseL expression in PIE cells, this effect was not similar with purified APS or NPS from the OLL1073R-1 strain.…”

Section: Discussionmentioning

confidence: 99%

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Genomic Characterization of Lactobacillus delbrueckii TUA4408L and Evaluation of the Antiviral Activities of its Extracellular Polysaccharides in Porcine Intestinal Epithelial Cells

et al. 2018

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In lactic acid bacteria, the synthesis of exopolysaccharides (EPS) has been associated with some favorable technological properties as well as health-promoting benefits. Research works have shown the potential of EPS produced by lactobacilli to differentially modulate immune responses. However, most studies were performed in immune cells and few works have concentrated in the immunomodulatory activities of EPS in non-immune cells such as intestinal epithelial cells. In addition, the cellular and molecular mechanisms involved in the immunoregulatory effects of EPS have not been studied in detail. In this work, we have performed a genomic characterization of Lactobacillus delbrueckii subsp. delbrueckii TUA4408L and evaluated the immunomodulatory and antiviral properties of its acidic (APS) and neutral (NPS) EPS in porcine intestinal epithelial (PIE) cells. Whole genome sequencing allowed the analysis of the general features of L. delbrueckii TUA4408L genome as well as the characterization of its EPS genes. A typical EPS gene cluster was found in the TUA4408L genome consisting in five highly conserved genes epsA-E, and a variable region, which includes the genes for the polymerase wzy, the flippase wzx, and seven glycosyltransferases. In addition, we demonstrated here for the first time that L. delbrueckii TUA4408L and its EPS are able to improve the resistance of PIE cells against rotavirus infection by reducing viral replication and regulating inflammatory response. Moreover, studies in PIE cells demonstrated that the TUA4408L strain and its EPS differentially modulate the antiviral innate immune response triggered by the activation of Toll-like receptor 3 (TLR3). L. delbrueckii TUA4408L and its EPS are capable of increasing the activation of interferon regulatory factor (IRF)-3 and nuclear factor κB (NF-κB) signaling pathways leading to an improved expression of the antiviral factors interferon (IFN)-β, Myxovirus resistance gene A (MxA) and RNaseL.

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Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Genomic Characterization of Lactobacillus delbrueckii TUA4408L and Evaluation of the Antiviral Activities of its Extracellular Polysaccharides in Porcine Intestinal Epithelial Cells

et al. 2018

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“…We rst explored the potential signaling pathway of B. subtilis and SEBS on mouse ileal crypt cells, then chicken crypt cells were employed. The innate immune system initiates a response to microorganisms via pattern recognition receptors, which have been extensively studied to explain the regulatory effects of immunobiotic bacteria in both immune and intestinal epithelial cells [49]. TLRs play a critical role in the recognition of pathogens by the innate immune system [50].…”

Section: Discussionmentioning

confidence: 99%

SEBS colonized in ileal mucosa, optimized bacterial composition, improved Se level and activated TLR2－NF-kB1 signaling pathway to regulate β-defensin 1 expression

Yang¹,

Wang²,

Zhang

et al. 2020

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Abstract Background Both selenium (Se) and probiotic bacillus improve immunity. Beta defensin 1 (BD1), a component of intestinal mucosal immunity, could be up-regulated in dietary selenium enriched bacillus subtilis (SEBS) supplementation. SEBS was supplemented in the culture medium of mouse intestinal crypt cells and the diets of chicks to observe the effects of SEBS on BD1 in the intestine by colonization of bacillus subtilis, its recognition and signaling pathway, bacterial composition optimization, and biological functions of Se. Results BD1 was formed in intestinal crypt cells and secreted into the lumen through the villi brush border. BD1 was up-regulated in distal ileum segments with SEBS and bacillus subtilis colonization. This occurred through the recognition of toll like receptor 2 and the NF-kB1 signaling pathway (TLR2-MyD88།NF-kB1), this increased expression was further enhanced with Se combination. Pro-inflammatory cytokines, TNF-α, IL-1β, and IL-6, were up-regulated with bacillus subtilis supplementation, while this up-regulation was inhibited with Se. Colonization of bacillus subtilis in distal segments of the ileum improved bacterial diversity, while reducing the number of endogenous Salmonella and lactobacilli sp. in ileal mucous membranes with SEBS supplementation. Species of unclassified Lachnospiraceae, uncultured Anaerosporobacter, Ruminococcaceae_UCG-014, Peptococcus, and Lactobacillus salivarius, and unclassified Butyricicoccus were substantial in ileal mucous membranes to promote BD1 concentration. Conclusion SECB, colonized in the ileal mucous membrane, optimized bacterial composition, enhanced BD1 secretion through activation of the TLR2-MyD88།NF-kB1 signaling pathway, and reduced pro-inflammatory factors. Our results suggest a new avenue for the combination of probiotic bacteria and essential micro-element selenium to improve intestinal mucosal immunity, increase defense against cold stress, and reduce illness incidence and mortality.

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“…Little is known about the PRRs involved in the recognition of EPS synthesized by probiotics. It has been shown that specific β-glucans could act as ligands for certain C-type lectins (such as Dectin 1) located in the intestinal epithelium [25]; additionally, specific heteropolymers synthesized by bifidobacteria or lactobacilli can be recognized by TLR (toll like receptor)-4 [24,26,27].…”

Section: Exopolysaccharides and Immunomodulationmentioning

confidence: 99%

Molecules Produced by Probiotics and Intestinal Microorganisms with Immunomodulatory Activity

et al. 2020

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Probiotics are live microorganisms that, when administered in adequate amounts, confer a health benefit on the host. The probiotic microorganisms most commonly used in the food and pharmacy industry belong to Lactobacillus and Bifidobacterium, and several strains of these genera have demonstrated beneficial attributes. In addition, some other intestinal bacteria inhabiting the human microbiota, such as Faecalibacterium prausnitzii and Akkermansia muciniphila, have recently been discovered and are able to display health-promoting effects in animal and human trials. The beneficial properties of probiotics have been known for a long time, although little is known about the molecular mechanisms and the molecules responsible for their effects. However, in recent years, advances in microbiome studies, and the use of novel analytical and molecular techniques have allowed a deeper insight into their effects at the molecular level. This review summarizes the current knowledge of some of the molecules of probiotics and other intestinal commensal bacteria responsible for their immunomodulatory effect, focusing on those with more solid scientific evidence.

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Exopolysaccharides from Lactobacillus delbrueckii OLL1073R-1 modulate innate antiviral immune response in porcine intestinal epithelial cells

Cited by 84 publications

References 33 publications

Genomic Characterization of Lactobacillus delbrueckii TUA4408L and Evaluation of the Antiviral Activities of its Extracellular Polysaccharides in Porcine Intestinal Epithelial Cells

Genomic Characterization of Lactobacillus delbrueckii TUA4408L and Evaluation of the Antiviral Activities of its Extracellular Polysaccharides in Porcine Intestinal Epithelial Cells

SEBS colonized in ileal mucosa, optimized bacterial composition, improved Se level and activated TLR2－NF-kB1 signaling pathway to regulate β-defensin 1 expression

Molecules Produced by Probiotics and Intestinal Microorganisms with Immunomodulatory Activity

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