Exopolysaccharides from yeast: insight into optimal conditions for biosynthesis, chemical composition and functional properties &amp;#8211; review

Gientka, Iwona; Błażejak, Stanisław; Stasiak-Różańska, Lidia; Chlebowska-Śmigiel, Anna

doi:10.17306/j.afs.2015.4.29

Cited by 48 publications

(32 citation statements)

References 28 publications

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“…This difference may be due to the different medium components (including sources of carbon and nitrogen) which can affect the EPSs produced by the yeasts. 5 Golubev cultivated the yeast in a medium that contained only glucose, yeast extract and peptone water. The glucose (as carbon source) and yeast extract (as nitrogen source) were same with our study, but the fermentation medium used in our study also contained another source of nitrogen (ammonium sulfate) which is commonly used in yeast culture media to produce EPSs.…”

Section: Molecular Weight and Monosaccharide Composition Analysesmentioning

confidence: 99%

“…The glucose (as carbon source) and yeast extract (as nitrogen source) were same with our study, but the fermentation medium used in our study also contained another source of nitrogen (ammonium sulfate) which is commonly used in yeast culture media to produce EPSs. 1,5 So far, EPSs are produced by a considerable number of xylose and galactose with the ratios of 55.1:26.1:9.6:1.9 which had a molecular weight equal to 1010 kDa. 28 Breierová et al showed that the EPSs produced by Cryptococcus laurentii CCY 17-3-16 were rich in mannose and had three fractions with different molecular weight distribution.…”

Section: Molecular Weight and Monosaccharide Composition Analysesmentioning

confidence: 99%

“…1,2 monomer compositions, molecular weights and presence of functional groups in their chemical structures. 1,2,5 In addition to the mentioned descriptions, yeast EPSs have increasingly stimulated the interest in the food and pharmaceutical industries as an additive, bio-emulsifier, thickener and healing agent as well as may have a large potential markets in the mentioned industries. 1,5,6,9 EPSs possess unique properties that are highly determined by the type of microorganism and the production conditions 1 ; hence, each EPS requires a separate study to examine its biological activities.…”

Section: Introductionmentioning

confidence: 99%

“…Heretofore, several species of yeasts including Aureobasidium, Bullera, Candida, Cryptococcus, Debaryomyces, Lipomyces, Pichia, Pseudozyma, Sporobolomyces and Rhodotorula genera, are known for their ability to produce EPSs in laboratory culture conditions. 1,5 Nevertheless, many of them have not been explored or are under investigation.…”

Section: Introductionmentioning

confidence: 99%

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Characterization and Prospective Applications of the Exopolysaccharides Produced by Rhodosporidium babjevae

et al. 2020

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Purpose: Due to the potential industrial and therapeutic applications of the yeast exopolysaccharides (EPSs), there has been an increasing demand to assess these biopolymers with improved characteristics. This study aimed to characterize the EPSs from Rhodosporidium babjevae (ATCC 90942 and IBRC-M 30088) as well as to evaluate their possible antioxidant, emulsifying and antiproliferative activities. Methods: Rhodosporidium babjevae was cultured for 5 days and following isolation of supernatant, EPSs precipitated with adding of cold absolute ethanol and freeze-dried. The EPSs chemical structure was determined by FT-IR, SEM, HPLC-SEC and GC-MS. Additionally the solubility, water holding capacity and emulsifying activity of EPSs were evaluated. In vitro, antioxidant activity was investigated against DPPH, superoxide and hydroxyl radicals. Finally the EPSs consequence on the cell proliferation of human breast adenocarcinoma (MCF-7) and Madin-Darby canine kidney (MDCK) cell lines was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test. Results: R. babjevae excreted 1.6±0.2 g/L of the EPSs. The EPSs had three fractions with molecular weights of 1.02 ×106 , 5×105 and 2×105 Da. Mannose and glucose were found as the main monosaccharides of the EPSs (84:16 mol%, respectively). The EPSs exhibited emulsifying activity on sun flower oil. The scavenging activities were found to be dose-dependent and higher than hyaluronic acid. Significant difference among the EPSs treatments on the proliferation of MCF-7 and MDCK cell lines was not observed (P>0.05). Conclusion: These results show the interesting potential of the EPSs from R. babjevae as biocompatible compounds for using in food and pharmaceutical fields.

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Section: Molecular Weight and Monosaccharide Composition Analysesmentioning

confidence: 99%

Section: Molecular Weight and Monosaccharide Composition Analysesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Characterization and Prospective Applications of the Exopolysaccharides Produced by Rhodosporidium babjevae

et al. 2020

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“…Biopolymers like EPS were not only produced by bacteria, also produced by plants, algae, fungi and yeast. (Gientka et al 2015) (Dilna et al 2015). Exopolysaccharides (EPSs) are heavy in their molecular weight and biodegradable (Sanalibaba et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Biosynthesis of silver nanoparticles and exopolysaccharide using novel Thermophilic strain (Ts-1) ofBacillus amyloliquefaciens

Thota¹,

Sreelatha²

2020

Preprint

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In the developing world, Nanotechnology became an efficient method in therapeutics, antimicrobials, diagnostics, catalysis, microelectronics, and high sensitivity biomolecular detection. As well as on the other hand, Exopolysaccharides are biopolymers which are also widely used in food formulation, bio-flocculants, bioabsorbents, drug delivery agents. As the chemical methods of synthesizing nanoparticles and polymers are environmentally risky, costly, and toxic. In the present study, we focused on production, purification, and characterization of silver nanoparticles (AgNPs) and exopolysaccharide (EPS) by eco-friendly, extracellular biosynthetic methods using novel thermophilic Bacillus amyloliquefaciens strain Ts-1. This strain was isolated from soil samples by employing pour and spread plate techniques. After obtaining pure culture, the bacterium was used for the synthesis of AgNPs and EPS. Nanoparticles were synthesized from AgNO 3 by using reducing agents secreted by bacteria, and Exopolysaccharide biosynthesis is carried out in three steps by the organism in the presence of a carbon source. Synthesis of colloidal AgNPs and EPS was monitored by UV-Visible spectroscopy and Visual observation, respectively. SEM, Edax and FTIR were performed for the characterization of the AgNPs and EPS such as their size, morphology and composition and we also showed the catalytic activity of AgNps in degradation of methylene blue.

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Molecular Engineering of Bacterial Exopolysaccharide for Improved Properties

Fernandes

Deo

Kulkarni

2021

Microbial Exopolysaccharides as Novel and Significant Biomaterials

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Exopolysaccharides from yeast: insight into optimal conditions for biosynthesis, chemical composition and functional properties – review

Cited by 48 publications

References 28 publications

Characterization and Prospective Applications of the Exopolysaccharides Produced by Rhodosporidium babjevae

Characterization and Prospective Applications of the Exopolysaccharides Produced by Rhodosporidium babjevae

Biosynthesis of silver nanoparticles and exopolysaccharide using novel Thermophilic strain (Ts-1) ofBacillus amyloliquefaciens

Molecular Engineering of Bacterial Exopolysaccharide for Improved Properties

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