Exosomal and extracellular HMGB1 have opposite effects on SASH1 expression in rat astrocytes and glioma C6 cells

Ma, Chao; Chen, Han; Zhang, Siming; Yan, Yingying; Wu, Ronghua; Wang, Yongjun; Liu, Yan; Liu, Yang

doi:10.1016/j.bbrc.2019.08.057

Cited by 20 publications

(15 citation statements)

References 21 publications

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“…This increase in nuclear integrity likely results from the elevated expression of proteins actively involved in the repair of nuclear material, including H3 and HMGB 1 (Figure 6). Recently, H3 [47] and HMGB [48] proteins have been isolated and identified in the exosomes. In mammals, sperm DNA is wrapped around the core histone H3, [49].…”

Section: Discussionmentioning

confidence: 99%

Improved Post-Thaw Quality of Canine Semen after Treatment with Exosomes from Conditioned Medium of Adipose-Derived Mesenchymal Stem Cells

Qamar

Fang

Kim

et al. 2019

Animals

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Simple SummaryIn this study, we have tried to exploit the potential role of exosomes-derived from canine adipose-derived mesenchymal stem cells in the protection and repair of damaged sperm during cryopreservation. The treatment of sperm with an optimal level of exosomes resulted in improved post-thaw semen quality. This improvement was due to the exosomal proteins that protect the sperm against oxidative damage and induce the repair of membranes and chromatin. The effect of exosomal proteins was also confirmed by higher expression of genes related to the repair of membranes and chromatin accompanied by the lower expression of a gene associated with reactive oxygen species production.AbstractFreezing decreases sperm quality, ultimately affecting fertilizing ability. The repair of freeze-damaged sperm is considered crucial for improving post-thaw viability and fertility. We investigated the effects of exosomes derived from canine adipose-derived mesenchymal stem cells on dog sperm structure and function during cryopreservation. The pooled ejaculate was diluted with buffer, without (Control), or with exosomal proteins (25, 50, or 100 µg/mL). Using fresh semen, the determined optimal exosomal protein concentration was 50 µg/mL (Group 2) which was used in further experiments. Post-thaw sperm treated with exosomes were superior to control (p < 0.05) in terms of motility (56.8 ± 0.3% vs. 47.2 ± 0.3%), live sperm percentage (55.9 ± 0.4% vs. 45.4 ± 0.4%), membrane integrity (55.6 ± 0.5% vs. 47.8 ± 0.3%), and acrosome integrity (60.4 ± 1.1% vs. 48.6 ± 0.4%). Moreover, expression of genes related to the repair of the plasma membrane (ANX 1, FN 1, and DYSF), and chromatin material (H3, and HMGB 1) was statistically higher in exosome-treated sperm than control, but the expression of the mitochondrial reactive oxygen species modulator 1 gene was significantly higher in control. Therefore, exosomal treatment may improve the quality of post-thaw dog semen through initiating damaged sperm repair and decreasing reactive oxygen species production.

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Section: Discussionmentioning

confidence: 99%

Improved Post-Thaw Quality of Canine Semen after Treatment with Exosomes from Conditioned Medium of Adipose-Derived Mesenchymal Stem Cells

Qamar

Fang

Kim

et al. 2019

Animals

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“…Ma et al, studied various protein expression patterns of normal glial cell and glioma-derived exosomes, and the effects of SASH1 gene expression in glioma [201]. They isolated exosomes from astrocytes and C6 cells, and identified their exosomal proteins using mass spectrometry.…”

Section: Exosomal Proteins In Gliomamentioning

confidence: 99%

“…However, the aforementioned process, which was suggested to be related to TLR4 signaling, needs more research. The structure-dependent function of the secreted protein HMGB1 to stimulate or suppress tumorigenesis, opens a new horizon for understanding the interaction between tumor cells and their microenvironment [201].…”

Section: Exosomal Proteins In Gliomamentioning

confidence: 99%

Role of exosomes in malignant glioma: microRNAs and proteins in pathogenesis and diagnosis

Mahjoubin‐Tehran

Movahedpour

et al. 2020

Cell Commun Signal

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Malignant gliomas are the most common and deadly type of central nervous system tumors. Despite some advances in treatment, the mean survival time remains only about 1.25 years. Even after surgery, radiotherapy and chemotherapy, gliomas still have a poor prognosis. Exosomes are the most common type of extracellular vesicles with a size range of 30 to 100 nm, and can act as carriers of proteins, RNAs, and other bioactive molecules. Exosomes play a key role in tumorigenesis and resistance to chemotherapy or radiation. Recent evidence has shown that exosomal microRNAs (miRNAs) can be detected in the extracellular microenvironment, and can also be transferred from cell to cell via exosome secretion and uptake. Therefore, many recent studies have focused on exosomal miRNAs as important cellular regulators in various physiological and pathological conditions. A variety of exosomal miRNAs have been implicated in the initiation and progression of gliomas, by activating and/or inhibiting different signaling pathways. Exosomal miRNAs could be used as therapeutic agents to modulate different biological processes in gliomas. Exosomal miRNAs derived from mesenchymal stem cells could also be used for glioma treatment. The present review summarizes the exosomal miRNAs that have been implicated in the pathogenesis, diagnosis and treatment of gliomas. Moreover, exosomal proteins could also be involved in glioma pathogenesis. Exosomal miRNAs and proteins could also serve as non-invasive biomarkers for prognosis and disease monitoring.

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“…Another study showed that a tumor suppressor, SAM, and SH3 domain-containing protein 1 (SASH1), was dysregulated or absent in glioma cells. Similar studies have also demonstrated the difference between the protein patterns of normal and glioma cells and the opposite effect of extracellular high-mobility group protein 1 (HMGB1) and astrocytic HMGB1 on SASH1 gene expression in one glioma cell line ( Ma et al, 2019 ). Interaction between astrocyte-derived exosomes that contain neurotrophic induced cargoes such as co-chaperone stress-inducible protein 1 (STI1), and neuronal surface components could also enhance their pathological signaling ( Hajj et al, 2013 ); STI1 also induced glioma through different pathways and is involved in neuronal death and neurodegenerative diseases ( Erlich et al, 2007 ; Landemberger et al, 2018 ).…”

Section: Astrocytes Evs and Cns Diseasesmentioning

confidence: 65%

The Function of Astrocyte Mediated Extracellular Vesicles in Central Nervous System Diseases

Gharbi

Zhang

Yang

2020

Front. Cell Dev. Biol.

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Astrocyte activation plays an important role during disease-induced inflammatory response in the brain. Exosomes in the brain could be released from bone marrow (BM)derived stem cells, neuro stem cells (NSC), mesenchymal stem cells (MSC), etc. We summarized that exosomes release and transport signaling to the target cells, and then produce function. Furthermore, we discussed the pathological interactions between astrocytes and other brain cells, which are related to brain diseases such as stroke, Alzheimer's disease (AD), Parkinson's disease (PD), amyotrophic lateral sclerosis (ALS) disease, multiple sclerosis (MS), psychiatric, traumatic brain injury (TBI), etc. We provide up-to-date, comprehensive and valuable information on the involvement of exosomes in brain diseases, which is beneficial for basic researchers and clinical physicians.

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Exosomal and extracellular HMGB1 have opposite effects on SASH1 expression in rat astrocytes and glioma C6 cells

Cited by 20 publications

References 21 publications

Improved Post-Thaw Quality of Canine Semen after Treatment with Exosomes from Conditioned Medium of Adipose-Derived Mesenchymal Stem Cells

Improved Post-Thaw Quality of Canine Semen after Treatment with Exosomes from Conditioned Medium of Adipose-Derived Mesenchymal Stem Cells

Role of exosomes in malignant glioma: microRNAs and proteins in pathogenesis and diagnosis

The Function of Astrocyte Mediated Extracellular Vesicles in Central Nervous System Diseases

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