Exosomal lncRNA CHL1-AS1 Derived from Peritoneal Macrophages Promotes the Progression of Endometriosis via the miR-610/MDM2 Axis

Liu, Ting; Liu, Mei; Zheng, Chunwen; Zhang, Daoyan; Li, Mingbao; Zhang, Lu

doi:10.2147/ijn.s323671

Cited by 21 publications

(27 citation statements)

References 36 publications

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“…Endometriosis related EVs were isolated from peritoneal fluid [ 18 , 23 , 27 , 28 , 30 , 33 , 46 ], endometrium [ 18 – 20 , 25 , 28 , 29 , 34 , 35 , 37 , 38 , 41 , 42 , 44 , 47 ], endometriosis lesions [ 18 , 19 , 26 , 28 ], endometriomas [ 25 , 30 , 34 , 35 , 39 – 42 , 44 , 47 ], cervicovaginal swabs (in a single monkey) [ 32 ], plasma [ 18 , 31 ] and serum [ 18 , 26 , 28 , 31 , 34 – 36 , 39 , 43 , 45 , 47 ]. (Plasma is recommended by the ISEV over serum, due to the release of a significant amount of additional EVs from platelets during the clot formation when preparing serum [ 48 ]).…”

Section: Resultsmentioning

confidence: 99%

“…Control samples not clearly stated in methods Lee 2021 [ 27 ] Human ( n = 45) Human ( n = 45) Peritoneal fluid UC Partially Microbiota composition of EVs Not discussed, but potential diagnostic targets Not discussed Microbiome of peritoneal fluid and/or genital tract may influence development of endometriosis May be specific to women with endometrioma Li 2020 [ 28 ] Human (serum n = 48) Human (serum n = 21) CCM of ESCs from endometrium and endometriosis lesions, serum CCM: ExoQuick-TC Exosome Precipitation Solution (#EXOTC10A-1, SBI) Serum: SEC Yes VEGF-C Serum EV VEGF-C provided sensitivity of 81.3%, and specificity of 71.4% Potential therapeutic target (e.g. with levatinib) EV secreted VEGF-C induces lymphangiogenesis and pro inflammatory microenvironment to promote endometriosis progression Number of participant samples used for CCM EV assays unclear Li 2021 [ 29 ] Human ( n = 23) Mouse ( n = 18) Human ( n = 10) Mouse ( n = 9) CCM of M1 macrophages from endometrial biopsy UC Partially Not investigated Treatment of endometriosis with M1NVs (M1 macrophage derived EVs) safe in mouse model M1NVs inhibit angiogenesis, migration and invasion of endometriosis, via reprogramming of M2 macrophage phenotype EV cargo not investigated – mechanism of reprogramming unknown Liu 2021 [ 30 ] Human ( n = 50) Human ( n = 50) CCM of primary peritoneal macrophages isolated from peritoneal fluid UC …”

Section: Methodsmentioning

confidence: 99%

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A promising future for endometriosis diagnosis and therapy: extracellular vesicles - a systematic review

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Paterson

Henry

2022

Reprod Biol Endocrinol

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Endometriosis is a chronic, inflammatory gynaecological disease that can have severe negative impacts on quality of life and fertility, placing burden on patients and the healthcare system. Due to the heterogeneous nature of endometriosis, and the lack of correlation between symptom and surgical disease severity, diagnosis and treatment remain a significant clinical challenge. Extracellular vesicles (EVs) are biologically active particles containing molecular cargo involved in intercellular communication, that can be exploited for diagnostic and therapeutic purposes.We systematically reviewed studies exploring EVs and their role in endometriosis, specifically addressing diagnostic and therapeutic potential and current understanding of pathophysiology. Five databases (Pubmed, Embase, Medline, Web of Science, Google Scholar) were searched for keywords ‘endometriosis’ and either ‘extracellular vesicles’ or ‘exosomes’.There were 28 studies included in the review. Endometrium derived EVs contribute to the development of endometriosis. EVs derived from endometriosis lesions contribute to angiogenesis, immunomodulation and fibrosis. Such EVs can be detected in blood, with early data demonstrating utility in diagnosis and recurrence detection. EV isolation techniques varied between studies and only eight of twenty-eight studies fully characterised EVs according to current recommended standards. Reporting/type of endometriosis was limited across studies. Varied patient population, type of sample and isolation techniques created bias and difficulty in comparing studies.EVs hold promise for improving care for symptomatic patients who have never had surgery, as well as those with recurrent symptoms after previous surgery. We encourage further EV research in endometriosis with the inclusion of rigorous reporting of both the patient population and technical methodology used, with the ultimate goal of achieving clinical utility for diagnosis, prognosis and eventually treatment.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

A promising future for endometriosis diagnosis and therapy: extracellular vesicles - a systematic review

Scheck

Paterson

Henry

2022

Reprod Biol Endocrinol

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“…We found that thrombospondin 1 (Thbs1), tissue inhibitor of metalloproteinase 1 (Timp1), and cell adhesion molecule with homology to L1CAM (Chl1) were up-regulated in both the model and patients. These genes are known to play a role in cell adhesion and/or ECM organization, biological processes important for the attachment and invasion of ectopic cells in tissues [14][15][16]. Thus, these genes might be critical for the development of endometriosis via cell attachment and invasion in both model and patients.…”

Section: Discussionmentioning

confidence: 99%

Genes Relating to Biological Processes of Endometriosis: Expression Changes Common to a Mouse Model and Patients

Iwasaki

Kaneda

2022

Drug Res (Stuttg)

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Endometriosis is one of the most common gynecological diseases in women of reproductive age. Retrograde menstruation is considered a major reason for the development of endometriosis. The syngeneic transplantation mouse model is an endometriosis animal model that is considered to mimic retrograde menstruation. However, it remains poorly understood which genetic signatures of endometriosis are reflected in this model. Here, we employed an in vivo syngeneic mouse endometriosis model and identified differentially expressed genes (DEGs) between the ectopic and eutopic tissues using microarray analysis. Three gene expression profile datasets, GSE5108, GSE7305, and GSE11691, were downloaded from the Gene Expression Omnibus database and DEGs between ectopic and eutopic tissues from the same patients were identified. Gene ontology analysis of the DEGs revealed that biological processes including cell adhesion, the inflammatory response, the response to mechanical stimulus, cell proliferation, and extracellular matrix organization were enriched in both the model and patients. Of the 195 DEGs common to the model and patients, 154 showed the same expression pattern, and 28 of these 154 DEGs came up when PubMed was searched for each gene along with the terms “endometriosis” and “development”. This is the first comparison of the DEGs of the mouse syngeneic endometriosis model and those of patients, and we identified the biological processes common to the model and patients at the transcriptional level. This model may be useful to evaluate the efficacy of drugs which target these biological processes.

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“…The overexpression of MDM2 was previously reported as a contributor to endometriosis progression by promoting proliferation, migration, and invasion and inhibiting apoptosis [ 25 ]. Another study by Liu et al revealed the axis linking MDM2 to endometriosis pathogenesis via miR-610 in the ectopic endometrial stromal cells, which introduced MDM2 as a potential therapeutic target for endometriosis [ 26 ]. The SLC6A8 gene is a creatine transporter that shows overexpression during the secretory phase in stromal cells and endometrial glands [ 27 ].…”

Section: Discussionmentioning

confidence: 99%

The Role of miRNAs 340-5p, 92a-3p, and 381-3p in Patients with Endometriosis: A Plasma and Mesenchymal Stem-Like Cell Study

Bahramy

Zafari

Izadi

et al. 2021

BioMed Research International

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Background. Endometriosis is the most prevalent gynecological disease with elusive etiology. The mysterious entity and the lack of noninvasive diagnostic methods affect women’s lives negatively. This study is aimed at finding the relationship between miR-340-5p, 92a-3p, and miR-381-3p and the pathogenesis of endometriosis in endometrial mesenchymal stem-like cells (eMSCs) of endometriosis and assessing their potential as a noninvasive biomarker in plasma. Methods. Peripheral blood and eMSC specimens were collected from suspected women of endometriosis before laparoscopy. Total RNA was isolated from plasma and cultured eMSCs to synthesize complementary DNA. The expression of miR-340-5p, miR-92a-3p, and miR-381-3p was analyzed by RT-qPCR. To understand these miRNAs’ role, we also did a bioinformatic analysis. Results. There was a downregulation of miR-340-5p, miR-92a-3p, and miR-381-3p in plasma, and the upregulation of miR-340-5p and the downregulation of miR-92a-3p and miR-381-3p in eMSCs of women with endometriosis. There was a positive concordance between the expression of miR-92a-3p and miR-381-3p in plasma and eMSCs. Our study also showed three genes, Solute Carrier Family 6 Member 8 (SLC6A8), Zinc Finger Protein 264 (ZNF264), and mouse double minute 2 (MDM2), as common targets of these miRNAs. Conclusions. This study has been one of the first attempts to examine the expression of miR-340-5p, miR-92a-3p, and miR-381-3p in both plasma and eMSCs and revealed their possible role in endometriosis based on in silico analysis. Biomarkers pave the way to develop a new therapeutic approach to the management or treatment of endometriosis patients. Our result as a first report shows that combined levels of miRNAs 340-5p and 381-3p may have the potential to be utilized as diagnostic biomarkers for endometriosis.

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Exosomal lncRNA CHL1-AS1 Derived from Peritoneal Macrophages Promotes the Progression of Endometriosis via the miR-610/MDM2 Axis

Cited by 21 publications

References 36 publications

A promising future for endometriosis diagnosis and therapy: extracellular vesicles - a systematic review

A promising future for endometriosis diagnosis and therapy: extracellular vesicles - a systematic review

Genes Relating to Biological Processes of Endometriosis: Expression Changes Common to a Mouse Model and Patients

The Role of miRNAs 340-5p, 92a-3p, and 381-3p in Patients with Endometriosis: A Plasma and Mesenchymal Stem-Like Cell Study

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