Exosomal micro<scp>RNA</scp>s as tumor markers in epithelial ovarian cancer

Chi, Pan; Stevic, Ines; Müller, Volkmar; Ni, Qingtao; Oliveira‐Ferrer, Leticia; Pantel, Klaus; Schwarzenbach, Heidi

doi:10.1002/1878-0261.12371

Cited by 149 publications

(123 citation statements)

References 41 publications

Supporting

Mentioning

109

Contrasting

Unclassified

Order By: Relevance

“…Deb et al summarized the current knowledge regarding the role of miRNA expression in OC [27]. In addition, several previous studies have shown that stable circulating miRNAs play an increasingly important role in the diagnosis of OC [9][10][11][12][13][14][15]. However, to our knowledge, few reports have described miRNA levels in exosomes isolated from the plasma of OC patients [16][17][18][19].…”

Section: Discussionmentioning

confidence: 99%

Serum exosomal microRNA-34a as a potential biomarker in epithelial ovarian cancer

et al. 2020

View full text Add to dashboard Cite

Background: Ovarian cancer (OC) is a leading cause of cancer-related death in women, and thus an accurate diagnosis of the predisposition and its early detection is necessary. The aims of this study were to determine whether serum exosomal microRNA-34a (miR-34a) in ovarian cancer could be used as a potential biomarker. Methods: Exosomes from OC patients' serum were collected, and exosomal miRNAs were extracted. The relative expression of miR-34a was calculated from 58 OC samples by quantitative real-time polymerase chain reaction. Results: Serum exosomal miR-34a levels were significantly increased in early-stage OC patients compared with advanced-stage patients. Its levels were significantly lower in patients with lymph node metastasis than in those with no lymph node metastasis. Furthermore, its levels in the recurrence group were significantly lower than those in the recurrence-free group. Conclusions: Serum exosomal miR-34a could be a potential biomarker for improving the diagnostic efficiency of OC.

show abstract

Section: Discussionmentioning

confidence: 99%

Serum exosomal microRNA-34a as a potential biomarker in epithelial ovarian cancer

et al. 2020

View full text Add to dashboard Cite

show abstract

“…There are a lot of studies regarding association between miRNA and ovarian cancer [22][23][24][25]. Our study identi ed the different miRNAs related to angiogenesis that contributed to metastasis of ovarian cancer using high thoughtput technology.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-133a-5p Promotes Angiogenesis of Endothelial Progenitor Cells via TRIM59/Id1 in Ovarian Cancer

Teng

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Endothelial progenitor cells (EPCs) play an important role in tumor angiogenesis and growth. Our previous study has indicated that over-expressed inhibitor of DNA binding/differentiation 1 (Id1) in EPCs could promote EPCs proliferation, migration, and adhesion. In this study, we assessed the effect of MicroRNAs (miRNAs) on EPCs migration and angiogenesis and its signaling pathway in patients suffered from ovarian cancer (OC).Methods: We cultured peripheral circulating EPCs derived from 32 OC patients and 20 healthy control subjects, respectively. The miRNA profiles of EPCs in ovarian cancer patients were compared with that in healthy control subjects, and aberrantly expressed miRNAs in both groups were identified via miRNA microarray and clustering analysis. Among these miRNAs, miR-133a-5p was considered as one of the most important miRNAs, which biological function in EPCs has been investigated. Bioinformatic analysis combined with knockdown and overexpression of miR-133a-5p were used to identify its target protein.Results: An obviously downregulated expression level of miR-133a-5p has been seen in EPCs with ovarian cancer patients. Downregulated expression level of miR-133a-5p has been seen in ovarian cancer tissues and ovarian cancer cells (SKOV-3 and OVCAR-3). Downregulated of miR-133a-5p can increase TRIM59 expression, moreover, downregulated of miR-133a-5p further induce migration and angiogenesis via increase VEGF and Id1 in EPCs. MiR-133a-5p pro-angiogenesis would be diminished by TRIM59 knockdown. Additionally, increased TRIM59 also can promote EPCs migration and angiogenesis.Conclusions: The study found that miR-133a-5p was an important upstream factor regulated Id1/VEGF expression. Additionally, functional studies have revealed that TRIM59 was a direct target protein of miR-133a-5p, and TRIM59 silencing attenuated the role of miR-133a-5p in angiogenesis and Id1/VEGF expression. So we proposed that miR-133a-5p would be a new target for OC therapy.

show abstract

“…Several noncoding RNAs, such as CircMUC16 (17), lncRNA LUCAT1 (18) and lncRNA NORAD (19) promote ovarian cancer progression through miR-199a-3p and miR-199a-5p. Pan et al reported that miR-126 was underrepresented in exosomes from the plasma of epithelial ovarian cancer patients compared with that of healthy women (20). MiR-145 was shown to serve as an outstanding biomarker of ovarian cancer, since it is signi cantly downregulated in the sera of ovarian cancer patients compared to healthy women (21).…”

Section: Discussionmentioning

confidence: 99%

Identification and Functional Validation of Differentially Expressed Micrornas in Ascites-derived Ovarian Cancer Cells Compared With Primary Tumour Tissue

Jiang

Lyu

Liu

et al. 2020

Preprint

View full text Add to dashboard Cite

Purpose: Ovarian cancer, manifested by malignant ascites, is the most lethal gynaecological cancer. Suspended ascites-derived spheroids may contribute to ovarian cancer metastasis. MicroRNAs (miRNAs) are also associated with ovarian cancer metastasis. Here, we aimed to investigate the differentially expressed miRNAs (DE-miRNAs) in ascites-derived spheroids compared with primary tumour tissue, which may regulate ovarian cancer metastasis.Methods: The DE-miRNAs between ovarian cancer primary tumour tissues and ascites-derived spheroids were identified by GEO2R screening in dataset GSE65819. We used MiRTarBase and STRING to predict the target hub genes of DE-miRNAs and WebGestalt to perform functional analysis of hub genes. ALGGEN PROMO and TransmiR v2.0 were used to predict the common transcription factors (TFs) that potentially regulate DE-miRNAs expression. The observed differences in DE-miRNAs expression were validated with human ovarian cancer samples and ovarian cancer cell lines using PCR. The functions of DE-miRNAs on ovarian cancer progression were verified by transwell and angiogenesis assays.Results: Through bioinformatics screening and experimental validation, miR-199a-3p, miR-199b-3p, miR-199a-5p, miR-126-3p and miR-145-5p were identified as being significantly downregulated in ascites-derived spheroids compared with primary tumour tissues. In addition, TFAP2A was identified as a potentially common upstream TF regulating the expression of the abovementioned DE-miRNAs. The overexpression of miR-199a-3p, miR-199b-3p, miR-199a-5p could inhibit ovarian cancer invasion, and the overexpression of miR-145-5p could inhibit angiogenesis.Conclusion: The downregulated expression of miR-199a-3p, miR-199b-3p, miR-199a-5p, miR-126-3p and miR-145-5p in ascites-derived spheroids plays a key role in promoting ovarian cancer progression, which may represent novel molecules for targeted therapy for ovarian cancer.

show abstract

Exosomal microRNAs as tumor markers in epithelial ovarian cancer

Cited by 149 publications

References 41 publications

Serum exosomal microRNA-34a as a potential biomarker in epithelial ovarian cancer

Serum exosomal microRNA-34a as a potential biomarker in epithelial ovarian cancer

MicroRNA-133a-5p Promotes Angiogenesis of Endothelial Progenitor Cells via TRIM59/Id1 in Ovarian Cancer

Identification and Functional Validation of Differentially Expressed Micrornas in Ascites-derived Ovarian Cancer Cells Compared With Primary Tumour Tissue

Contact Info

Product

Resources

About