Exosomal microRNA expression profiles of cerebrospinal fluid in febrile seizure patients

Abstract

Febrile seizures (FS) are the most common seizures found in pediatric patients. Recently, microRNA (miRNA) have been used as a novel biomarker for the diagnosis of various diseases. This study aims to explore the exosomal miRNA expression profile of the cerebrospinal fluid (CSF) in atypical FS patients. Methods: This is a case-control study including CSF specimens of 41 pediatric patients. The CSF specimens were categorized into FS and a control group. Microarray assays were performed to evaluate the CSF exosomal miRNA expression profile. Quantitative PCR (qPCR) assays were conducted to validate the microarray assay result. Bioinformatic analysis was performed to analyze the result. Results: Thirteen (62%) patients in the FS group experienced complex FS. A total of 96 miRNAs were significantly expressed in the CSF study samples and 95 amongst them, exhibited higher expression in FS than in the control group. Further validation qPCR test indicated that the top 5 highly expressed miRNA (miR-4486, miR-6850-5p, miR-642b-3p, miR-7107-5p, miR-4281) showed same results as in the microarray assay. Bioinformatic analysis identified 455 target genes in the FS group. Conclusion: FS patients displayed higher CSF exosomal miRNA profiles than the control. These altered miRNA profiles appeared to be related to complex FS.

Expression of MicroRNA 146a, 155, 181 and 223 in febrile seizure

“…Lumbar puncture and cerebrospinal fluid (CSF) analysis are not recommended routinely to evaluate FS patients so was not conducted in the present study. In a recent study, Kim et al 23 searched the miRNA profile of CSF in FS patients. They reported 95 miRNAs were significantly higher in patients than in controls and the top 5 highly expressed miRNAs were miRNA-4486, miRNA-6850-5p, miRNA-642-3p, miRNA-7107-5p and miRNA-4281.…”

Promising candidate cerebrospinal fluid biomarkers of seizure disorder, infection, inflammation, tumor, and traumatic brain injury in pediatric patients

“…35) These highly expressed miRNAs included miR-4486, miR-6850-5p, miR-642b-3p, miR-7107-5p, and miR-4281 (Table 2). 35) 2. CNS infection and inflammation CSF/serum albumin ratio and CSF cell counts, culture, lactate and IgG or IgM measurements are basic CSF tests that have been used to diagnose infectious and inflammatory CNS disorders.…”

“…Few studies have shown a relationship between seizures and CSF miRNA. Furthermore, febrile seizure patients displayed higher CSF exosomal miRNA profiles than controls, and these altered miRNA profiles appeared related to complex febrile seizures [ 35 ]. These highly expressed miRNAs included miR-4486, miR-6850-5p, miR-642b-3p, miR-7107-5p, and miR-4281 ( Table 1 ) [ 35 ].…”

miR‑4486 reverses cisplatin‑resistance of colon cancer cells via targeting ATG7 to inhibiting autophagy

“…Additionally, miR-4486 was significantly downregulated in patients with chronic obstructive pulmonary disease with eosinophilia and was an indicator of poor prognosis ( 15 ). Furthermore, increased expression of miR-4486 was found to be associated with febrile seizures ( 16 ). However, to the best of our knowledge, the expression and effect of miR-4486 in CC have not been previously reported.…”