Exosomal miR‐6803‐5p as potential diagnostic and prognostic marker in colorectal cancer

Abstract

Accumulating data have suggested exosome-delivered microRNAs (miRNAs) play critical role in carcinogenesis and cancer progression. However, little is known about the influence of exosomal miR-6803-5p on the development and prognosis of colorectal cancer (CRC). Levels of serum exosomal miR-6803-5p were determined by microarray analysis and verified by quantitative real-time PCR (qRT-PCR). Outcomes of overall survival (OS) and disease-free survival (DFS) of CRC patients were estimated by Kaplan-Meier analysis. We used cox regression analysis to investigate the association between exosomes-encapsulated miR-6803-5p and the clinicopathological factors of CRC patients. The exosomal miR-6803-5p was significantly increased in serum samples from patients with CRC in contrast to healthy controls. Significantly higher levels of serum exosomal miR-6803-5p were observed in CRC patients at later TNM stage or with lymph node metastasis as well as liver metastasis. Patients with elevated levels of serum exosomal miR-6803-5p had much poorer OS and DFS. Cox regression analysis revealed high levels of exosomal miR-6803-5p was associated with poor prognosis in CRC independent of other confounding factors. Thus, exosomal miR-6803-5p is a potential diagnostic and prognostic biomarker for patients with CRC.

Perspectives of the Application of Liquid Biopsy in Colorectal Cancer

“…Serum extracellular miR-150-5p has been confirmed as an independent prognostic indicator of CRC, and the survival time of patients with a low expression of serum extracellular miR-150-5p was significantly longer than that of patients with high expression [134]. Similar results were obtained in serum, where high levels of exosomal miR-6803-5p have been shown to be associated with the poor prognosis of CRC [135]. Another study revealed that, compared with healthy individuals in the control group, the expression levels of miR-19a and miR-92a in the serum of patients with CRC are significantly increased, which is associated with CRC recurrence [136].…”

Profiling and functional analysis of differentially expressed circular RNAs in high glucose‐induced human umbilical vein endothelial cells

“…Some other predicted target miRNAs of hsa_circ_0000109 , including miR‐637 , miR‐3620‐5p , miR‐3064‐5p , and miR‐6803‐5p , have not been reported directly related to functional regulations of HUVECs. But these miRNAs have been unveiled to play central roles in the competing endogenous RNA (ceRNA) network in others diseases . However, they might be new targets in ceRNA network in vascular endothelium damage caused by high glucose.…”

The Impact of miRNA in Colorectal Cancer Progression and Its Liver Metastases<strong> </strong>

“…Moreover, recent evidence has suggested that liver pre-metastatic niche could be promoted by exosomal miR-21 of CRC cells, through the miR-21-TLR7-IL6 axis [116]. Significantly increased levels of serum exosomal miR-6803-5p [117] and decreased levels of miR-548c-5p have been recently associated with poor survival and liver metastasis of CRC patients [118]. However, the molecular mechanisms by which both miR-6803-5p and miR-548c-5p influence CRC metastasis remain largely unknown.…”