Exosome beads array for multiplexed phenotyping in cancer

Jara‐Acevedo, Ricardo; Campos-Silva, Carmen; Valés‐Gómez, Mar; Yáñez-Mó, Marı́a; Suárez, Henar; Fuentes, Manuel

doi:10.1016/j.jprot.2018.12.023

Cited by 20 publications

(17 citation statements)

References 60 publications

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“…Proteins have even been used as markers for the identification and isolation of exosomes, although the effect that the choice of isolation method can have on the observed protein profile must be also considered. [ 23 ]…”

Section: Exosome Compositionmentioning

confidence: 99%

“…In vivo exosomes are found in many biological fluids such as blood, urine, semen, breast milk, saliva, amniotic fluid, bile, bronchoalveolar lavage fluid, synovial fluid, and cerebrospinal fluid. [ 23,29 ]…”

Section: Formation Of Exosomesmentioning

confidence: 99%

“…It is estimated that up to 80% in vivo circulating exosomes are derived from platelets. [ 23,29,30 ]…”

Section: Formation Of Exosomesmentioning

confidence: 99%

“…[ 31 ] It has been demonstrated that the rate of exosome biogenesis is related to the stimuli or stress factors to which the secreting cell is submitted, and therefore to their physiological or pathological status. [ 23 ]…”

Section: Formation Of Exosomesmentioning

confidence: 99%

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Advances in Exosomes‐Based Drug Delivery Systems

Gutiérrez-Millán

Díaz

Lanao

et al. 2020

Macromolecular Bioscience

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Exosomes, a subgroup of extracellular vesicles, are important mediators of long‐distance intercellular communication and are involved in a diverse range of biological processes such as the transport of lipids, proteins, and nucleic acids. Researchers, seeing the problems caused by the toxic effects and clearance of synthetic nanoparticles, consider exosomes as an interesting alternative to such nanoparticles in the specific and controlled transport of drugs. In recent years, there have been remarkable advances in the use of exosomes in cancer therapeutics or for treating neurological diseases, among other applications. The objective of this work is to analyze studies focused on exosomes used in drug delivery system, present and future applications in this field of research are discussed based on the results obtained.

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Section: Exosome Compositionmentioning

confidence: 99%

Section: Formation Of Exosomesmentioning

confidence: 99%

“…It is estimated that up to 80% in vivo circulating exosomes are derived from platelets. [ 23,29,30 ]…”

Section: Formation Of Exosomesmentioning

confidence: 99%

Section: Formation Of Exosomesmentioning

confidence: 99%

See 2 more Smart Citations

Advances in Exosomes‐Based Drug Delivery Systems

Gutiérrez-Millán

Díaz

Lanao

et al. 2020

Macromolecular Bioscience

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“…Extracellular vesicles (EVs) were first found in 1946 by Chargaff and West EVs are secreted into the extracellular microenvironment by most cell types and can be further sorted into three subclasses based on their biogenesis: i) apoptotic bodies (1–5 µm), ii) microvesicles (150 nm to 1 µm), and iii) exosomes (30–150 nm in diameter) …”

Section: Exosomesmentioning

confidence: 99%

Progress in Microfluidics‐Based Exosome Separation and Detection Technologies for Diagnostic Applications

Lin

Chen

et al. 2019

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Exosomes are secreted by most cell types and circulate in body fluids. Recent studies have revealed that exosomes play a significant role in intercellular communication and are closely associated with the pathogenesis of disease. Therefore, exosomes are considered promising biomarkers for disease diagnosis. However, exosomes are always mixed with other components of body fluids. Consequently, separation methods for exosomes that allow high‐purity and high‐throughput separation with a high recovery rate and detection techniques for exosomes that are rapid, highly sensitive, highly specific, and have a low detection limit are indispensable for diagnostic applications. For decades, many exosome separation and detection techniques have been developed to achieve the aforementioned goals. However, in most cases, these two techniques are performed separately, which increases operation complexity, time consumption, and cost. The emergence of microfluidics offers a promising way to integrate exosome separation and detection functions into a single chip. Herein, an overview of conventional and microfluidics‐based techniques for exosome separation and detection is presented. Moreover, the advantages and drawbacks of these techniques are compared.

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From Conventional to Microfluidic: Progress in Extracellular Vesicle Separation and Individual Characterization

Chen

Lin

Cheng

et al. 2023

Adv Healthcare Materials

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Extracellular vesicles (EVs) are nanoscale membrane vesicles, which contain a wide variety of cargo such as proteins, miRNAs, and lipids. A growing body of evidence suggests that EVs are promising biomarkers for disease diagnosis and therapeutic strategies. Although the excellent clinical value, their use in personalized healthcare practice is not yet feasible due to their highly heterogeneous nature. Taking the difficulty of isolation and the small size of EVs into account, the characterization of EVs at a single‐particle level is both imperative and challenging. In a bid to address this critical point, more research has been directed into a microfluidic platform because of its inherent advantages in sensitivity, specificity, and throughput. This review discusses the biogenesis and heterogeneity of EVs and takes a broad view of state‐of‐the‐art advances in microfluidics‐based EV research, including not only EV separation, but also the single EV characterization of biophysical detection and biochemical analysis. To highlight the advantages of microfluidic techniques, conventional technologies are included for comparison. The current status of artificial intelligence (AI) for single EV characterization is then presented. Furthermore, the challenges and prospects of microfluidics and its combination with AI applications in single EV characterization are also discussed. In the foreseeable future, recent breakthroughs in microfluidic platforms are expected to pave the way for single EV analysis and improve applications for precision medicine.

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Exosome beads array for multiplexed phenotyping in cancer

Cited by 20 publications

References 60 publications

Advances in Exosomes‐Based Drug Delivery Systems

Advances in Exosomes‐Based Drug Delivery Systems

Progress in Microfluidics‐Based Exosome Separation and Detection Technologies for Diagnostic Applications

From Conventional to Microfluidic: Progress in Extracellular Vesicle Separation and Individual Characterization

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