Exosome-Derived LINC00960 and LINC02470 Promote the Epithelial-Mesenchymal Transition and Aggressiveness of Bladder Cancer Cells

Huang, Chengshuo; Ho, Jar-Yi; Chiang, Jung-Hwa; Yu, Cheng‐Ping; Yü, Dah‐Shyong

doi:10.3390/cells9061419

Cited by 46 publications

(41 citation statements)

References 57 publications

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“…Reportedly, LINC00960 expression is up-regulated in the fibroblasts of idiopathic pulmonary fibrosis, and LINC00960 negatively regulates its proliferation [25]. A recent study reports that, through up-regulating βcatenin signal, Notch signal, and Smad2/3 signal, exosome-derived LINC00960 can promote the malignant behaviors of low-grade bladder cancer cells and induce epithelial-mesenchymal transition, thus working as a potential liquid biomarker to monitor the progression of bladder cancer [26]. This study found that LINC00960 expression was up-regulated in PDAC, which is consistent with the previous report [16].…”

Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE: Long intergenic non-protein coding RNA 960 regulates cancer cell viability, migration and invasion through modulating miR-146a-5p/interleukin 1 receptor associated kinase 1 axis in pancreatic ductal adenocarcinoma

Huang

et al. 2021

Bioengineered

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Long non-coding RNAs (lncRNAs) are considered as crucial regulatory factors in cancer biology. However, the biological function of long intergenic non-protein coding RNA 960 (LINC00960) in the tumorigenesis of pancreatic ductal adenocarcinoma (PDAC) is still unknown. The goal of this study is to investigate the role of LINC00960 in PDAC. Quantitative real‐time polymerase chain reaction (qRT-PCR) was used to examine the expression levels of LINC00960 in PDAC tissues and cell lines. After transfection, the loss-of-function models of LINC00960 or interleukin 1 receptor-associated kinase 1 (IRAK1) were established with BxPC-3 cells and Colo357 cells, and the malignant phenotypes of BxPC-3 cells and Colo357 cells were detected by CCK-8 assay, BrdU assay and Transwell assay, respectively. The interactions among LINC00960, miR-146a-5p and IRAK1 were predicted by bioinformatics analysis, and verified by luciferase reporter assay, RNA immunoprecipitation assay and RNA pull-down assay. The regulatory functions of LINC00960 and miR-146a-5p on IRAK1 were detected by Western blot. We demonstrated that the LINC00960 expression was increased in PDAC tissues and cell lines. Knocking down LINC00960 or IRAK1 could repress the viability, migration, and invasion of BxPC-3 and Colo357 cells. LINC00960 functioned as a molecular sponge for miR-146a-5p, and IRAK1 was verified as a target gene of miR-146a-5p. Additionally, LINC00960 could up-regulate IRAK1 expression via repressing miR-146a-5p, and the oncogenic properties of LINC00960 were partly reversed by miR-146a-5p. Our findings reveal that LINC00960 is a promoter of PDAC progression through regulating miR-146a-5p/IRAK1axis.

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Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE: Long intergenic non-protein coding RNA 960 regulates cancer cell viability, migration and invasion through modulating miR-146a-5p/interleukin 1 receptor associated kinase 1 axis in pancreatic ductal adenocarcinoma

Huang

et al. 2021

Bioengineered

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“…pointed out that exosomal lnc_00960 and lnc_02470 promoted EMT and the aggressiveness of BCa cells. They treated BCa TSGH-8301 cells with exosomes derived from BCa T24 or J82 cells, leading to decreased E-cadherin levels and increased N-cadherin, vimentin, MMP2, and MMP9 levels in TSGH-8301 cells ( 66 ). Berrondo et al.…”

Section: The Biological Roles Of Exosome-derived Ncrnas In Bcamentioning

confidence: 99%

Biological Roles and Clinical Significance of Exosome-Derived Noncoding RNAs in Bladder Cancer

et al. 2021

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Bladder cancer (BCa) is a common heterogeneous urinary system tumor with high malignancy and limited advancement in treatment. Limited understanding of BCa has not contributed to any significant progress in diagnosis or treatment, exploring the mechanisms underlying BCa has become an urgent research focus. Exosomes, a type of extracellular vesicle (EV), have drawn substantial interest for their important roles in mediating intracellular communication. Exosomes shuttle numerous bioactive molecules, and noncoding RNAs (ncRNAs) are among the most numerous. ncRNAs including microRNA, long noncoding RNA, and circular RNA are sorted and packaged into exosomes selectively and transferred into recipient cells to regulate their function. Exosomal ncRNAs are associated with hallmarks of BCa, such as proliferation, apoptosis, epithelial-mesenchymal transition (EMT), cell cycle arrest, lymphangiogenesis, and chemotherapy resistance. Exosomal ncRNAs can also be detected in urine and serum, making them encouraging biomarkers for BCa diagnosis and prognosis. More importantly, exosomes exhibit excellent biocompatibility and potential for diversified applications. The delivery of bioactive substances and drugs into specific cells has become a promising approach for precision therapy for BCa patients. In addition, cancer vaccines have also received increasing attention. In this review, we summarize the current research on the regulatory roles of exosomal ncRNAs in BCa tumorigenesis and progression, as well as their potential clinical value in accelerating the diagnosis and therapy of BCa.

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“…Exosomes are vesicles of endocytic origin and continuously released from the bladder [91], which can mediate communication between cells through the transfer of nucleic acids [92][93][94][95] and proteins [96] in BC. Numerous studies have been conducted to investigate the role of exosomes in patients with BC.…”

Section: Circulating Mirnas and Othersmentioning

confidence: 99%

Tumor heterogeneity and the potential role of liquid biopsy in bladder cancer

Huang

2020

Cancer Communications

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Bladder cancer (BC) is a heterogeneous disease that characterized by genomic instability and a high mutation rate. Heterogeneity in tumor may partially explain the diversity of responses to targeted therapies and the various clinical outcomes. A combination of cytology and cystoscopy is the standard methodology for BC diagnosis, prognosis, and disease surveillance. However, genomics analyses of single tumor-biopsy specimens may underestimate the mutational burden of heterogeneous tumors. Liquid biopsy, as a promising technology, enables analysis of tumor components in the bodily fluids, such as blood and urine, at multiple time points and provides a minimally invasive approach that can track the evolutionary dynamics and monitor tumor heterogeneity. In this review, we describe the multiple faces of BC heterogeneity at the genomic and transcriptional levels and how they affect clinical care and outcomes. We also summarize the outcomes of liquid biopsy in BC, which plays a potential role in revealing tumor heterogeneity. Finally, we discuss the challenges that must be addressed before liquid biopsy can be widely used in clinical treatment.

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Exosome-Derived LINC00960 and LINC02470 Promote the Epithelial-Mesenchymal Transition and Aggressiveness of Bladder Cancer Cells

Cited by 46 publications

References 57 publications

RETRACTED ARTICLE: Long intergenic non-protein coding RNA 960 regulates cancer cell viability, migration and invasion through modulating miR-146a-5p/interleukin 1 receptor associated kinase 1 axis in pancreatic ductal adenocarcinoma

RETRACTED ARTICLE: Long intergenic non-protein coding RNA 960 regulates cancer cell viability, migration and invasion through modulating miR-146a-5p/interleukin 1 receptor associated kinase 1 axis in pancreatic ductal adenocarcinoma

Biological Roles and Clinical Significance of Exosome-Derived Noncoding RNAs in Bladder Cancer

Tumor heterogeneity and the potential role of liquid biopsy in bladder cancer

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