Exosome-mediated miR-1290 regulation of SLU7 affects hepatocellular carcinoma process

Abstract

Objective: Liver cancer, as a very common malignant tumor in clinic, has a high incidence rate and a poor prognosis. Understanding the relationship of exosomes miR-1290 and SLU7 with liver cancer may be a breakthrough in the diagnosis and treatment of liver cancer in the future.Methods: Altogether 64 patients with liver cancer and 58 healthy people in Shandong Cancer Hospital affiliated to Shandong University were collected, and liver cancer cells were purchased. qPCR was used to detect the mRNA levels of miR-1290 and SLU7. Western blot was used to detect the relative expression of SLU7 protein, and the effect of exosomes miR-1290 and SLU7 on the biological behavior of liver cancer cells was analyzed. Double fluorescein reporter was used to verify the targeted relationship between miR-1290 and SLU7.Results: miR-1290 was highly expressed in liver cancer (P< 0.050) and SLU7 was poorly expressed in liver cancer (P< 0.050). Exosomes miR-1290 could inhibit the relative expression of SLU7 protein (p < 0.050), promote the proliferation, invasion, migration and epithelial-mesenchymal transformation of liver cancer cells, and reduce the ability of apoptosis (P< 0.050). Double luciferase report and rescue experiment confirmed that miR-1290 and SLU7 had targeted relationship (P< 0.050).Conclusion: Exosomes miR-1290 may be a potential target for the diagnosis and treatment of liver cancer in the future through targeting inhibition of SLU7 in the occurrence and development of liver cancer.