Exosome Research and Co-culture Study

Shimasaki, Takeo; Yamamoto, Shuichi; Arisawa, Tomiyasu

doi:10.1248/bpb.b18-00223

Cited by 26 publications

(24 citation statements)

References 74 publications

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“…These cells were interactively co-cultured on NICO-1 plates (Ginrei Lab Inc, Ishikawa, Japan). NICO-1 plates are horizontal co-culture plates in which the humoral factor can move freely through the filter but the cells cannot [23]. We prepared two types of HBdSF cells transfected with negative control siRNA or DAB2 siRNA.…”

Section: Interactive Co-culture Of Urothelial Cancer Cells and Bladdementioning

confidence: 99%

Disabled Homolog 2 (DAB2) Protein in Tumor Microenvironment Correlates with Aggressive Phenotype in Human Urothelial Carcinoma of the Bladder

et al. 2020

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Disabled homolog-2 (DAB2) has been reported to be a tumor suppressor gene. However, a number of contrary studies suggested that DAB2 promotes tumor invasion in urothelial carcinoma of the bladder (UCB). Here, we investigated the clinical role and biological function of DAB2 in human UCB. Immunohistochemical staining analysis for DAB2 was carried out on UCB tissue specimens. DAB2 expression levels were compared with clinicopathological factors. DAB2 was knocked-down by small interfering RNA (siRNA) transfection, and then its effects on cell proliferation, invasion, and migration, and changes to epithelial-mesenchymal transition (EMT)-related proteins were evaluated. In our in vivo assays, tumor-bearing athymic nude mice subcutaneously inoculated with human UCB cells (MGH-U-3 or UM-UC-3) were treated by DAB2-targeting siRNA. Higher expression of DAB2 was associated with higher clinical T category, high tumor grade, and poor oncological outcome. The knock-down of DAB2 decreased both invasion and migration ability and expression of EMT-related proteins. Significant inhibitory effects on tumor growth and invasion were observed in xenograft tumors of UM-UC-3 treated by DAB2-targeting siRNA. Our findings suggested that DAB2 expression was associated with poor prognosis through increased oncogenic properties including tumor proliferation, migration, invasion, and enhancement of EMT in human UCB.

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Section: Interactive Co-culture Of Urothelial Cancer Cells and Bladdementioning

confidence: 99%

Disabled Homolog 2 (DAB2) Protein in Tumor Microenvironment Correlates with Aggressive Phenotype in Human Urothelial Carcinoma of the Bladder

et al. 2020

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“…The in ammatory factors were con rmed to signi cantly decrease in a short time in H:BM-M; therefore, we hypothesized that it would have been reduced by the factor possessed by EVs. In particular, in H:BM-M, it was hypothesized that the payload of a speci c factor increases, and some studies have reported that some of the miRNA levels may vary depending on the co-culture conditions [9,33,34].…”

Section: Increased Mirna Expression Level In Evs By Co-culture Conditionmentioning

confidence: 99%

Alteration of Payload in Extracellular Vesicles by Crosstalk With Mesenchymal Stem Cells From Different Origin

Park

Kong

Seo

2021

Preprint

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BackgroundThe application of extracellular vesicles (EVs) derived from mesenchymal stem cells (MSCs) requires customized materials to target disease or cell damage. We hypothesized that EVs exert different inflammatory effects on one recipient cell, although stem cells of different origins in humans have similar payloads.ResultsHere, the payload of EVs released by crosstalk between MSCs and human middle ear epithelial cells (HMEECs) extracted from adipose tissue, bone marrow and tonsils significantly increased the level of anti-inflammatory factors. EVs derived from the co-culture medium decreased TNF-α, COX-2, IL-1β, and IL-6 levels to approximately zero within 3 h in HMEECs. Expression of miR-638 and amyloid-β A4 precursor protein-binding family A member 2 was analyzed using microarrays and gene ontology analysis, respectively.ConclusionsIn conclusion, stem cells of different origins have different payloads through crosstalk with recipient-specific cells. Inducing specific factors in EVs by co-culture with MSCs could be valuable in regenerative medicine.

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“…These new techniques have exhibited high potential application prospects because of their simple, fast, and time‐saving procedures, as well as the minimal consumption of samples and reagents. Currently, although several reviews have reported and discussed the advances in exosomes, most have focused on specific aspects of the exosomes field, such as conventional exosome separation or enrichment techniques, the biological role of exosomes, and exosome detection techniques …”

Section: Introductionmentioning

confidence: 99%

“…Currently, although several reviews have reported and discussed the advances in exosomes, most have focused on specific aspects of the exosomes field, such as conventional exosome separation or enrichment techniques, the biological role of exosomes, and exosome detection techniques. [63][64][65][66] To better understand the significance of exosomes in diagnostic application fields, we will discuss the biogenesis and contents of exosomes. Progresses in exosome separation and detection techniques will also be reviewed based on certain representative exosome studies.…”

Section: Introductionmentioning

confidence: 99%

Progress in Microfluidics‐Based Exosome Separation and Detection Technologies for Diagnostic Applications

Lin

Chen

et al. 2019

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245

182

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Exosomes are secreted by most cell types and circulate in body fluids. Recent studies have revealed that exosomes play a significant role in intercellular communication and are closely associated with the pathogenesis of disease. Therefore, exosomes are considered promising biomarkers for disease diagnosis. However, exosomes are always mixed with other components of body fluids. Consequently, separation methods for exosomes that allow high‐purity and high‐throughput separation with a high recovery rate and detection techniques for exosomes that are rapid, highly sensitive, highly specific, and have a low detection limit are indispensable for diagnostic applications. For decades, many exosome separation and detection techniques have been developed to achieve the aforementioned goals. However, in most cases, these two techniques are performed separately, which increases operation complexity, time consumption, and cost. The emergence of microfluidics offers a promising way to integrate exosome separation and detection functions into a single chip. Herein, an overview of conventional and microfluidics‐based techniques for exosome separation and detection is presented. Moreover, the advantages and drawbacks of these techniques are compared.

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Exosome Research and Co-culture Study

Cited by 26 publications

References 74 publications

Disabled Homolog 2 (DAB2) Protein in Tumor Microenvironment Correlates with Aggressive Phenotype in Human Urothelial Carcinoma of the Bladder

Disabled Homolog 2 (DAB2) Protein in Tumor Microenvironment Correlates with Aggressive Phenotype in Human Urothelial Carcinoma of the Bladder

Alteration of Payload in Extracellular Vesicles by Crosstalk With Mesenchymal Stem Cells From Different Origin

Progress in Microfluidics‐Based Exosome Separation and Detection Technologies for Diagnostic Applications

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