Exosomes: biology, therapeutic potential, and emerging role in musculoskeletal repair and regeneration

Cobelli, Neil J.; Leong, Daniel J.; Sun, Hui

doi:10.1111/nyas.13469

“…Results derived from the use of conditioned medium may be due to the presence of EVs in addition to other factors. EVs are proposed to mediate intercellular communications and have been implicated in repair and disease processes by virtue of the cargo that they carry, which may include proteins, biofactors, and RNAs, including specific miRNAs, among others …”

Section: Key Question 2: What Is the Current Knowledge About The Thermentioning

confidence: 99%

American Society for Bone and Mineral Research‐Orthopaedic Research Society Joint Task Force Report on Cell‐Based Therapies – Secondary Publication

O’Keefe

¹

,

Tuan

²

,

Lane

³

et al. 2020

Journal Orthopaedic Research

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Cell‐based therapies, defined here as the delivery of cells in vivo to treat disease, have recently gained increasing public attention as a potentially promising approach to restore structure and function to musculoskeletal tissues. Although cell‐based therapy has the potential to improve the treatment of disorders of the musculoskeletal system, there is also the possibility of misuse and misrepresentation of the efficacy of such treatments. The medical literature contains anecdotal reports and research studies, along with web‐based marketing and patient testimonials supporting cell‐based therapy. Both the American Society for Bone and Mineral Research (ASBMR) and the Orthopaedic Research Society (ORS) are committed to ensuring that the potential of cell‐based therapies is realized through rigorous, reproducible, and clinically meaningful scientific discovery. The two organizations convened a multidisciplinary and international Task Force composed of physicians, surgeons, and scientists who are recognized experts in the development and use of cell‐based therapies. The Task Force was charged with defining the state‐of‐the art in cell‐based therapies and identifying the gaps in knowledge and methodologies that should guide the research agenda. The efforts of this Task Force are designed to provide researchers and clinicians with a better understanding of the current state of the science and research needed to advance the study and use of cell‐based therapies for skeletal tissues. The design and implementation of rigorous, thorough protocols will be critical to leveraging these innovative treatments and optimizing clinical and functional patient outcomes. In addition to providing specific recommendations and ethical considerations for preclinical and clinical investigations, this report concludes with an outline to address knowledge gaps in how to determine the cell autonomous and nonautonomous effects of a donor population used for bone regeneration. © 2020 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 38:485–502, 2020

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“…EVs are proposed to mediate intercellular communications and have been implicated in repair and disease processes by virtue of the cargo that they carry, which may include proteins, biofactors, and RNAs, including specific miRNAs, among others. (52,53) Key Question 3: What Is the Current Knowledge About Tissue-Specific Animal Models of Cell-Based Therapies?…”

Section: Conditioned Mediummentioning

confidence: 99%

American Society for Bone and Mineral Research-Orthopaedic Research Society Joint Task Force Report on Cell-Based Therapies

O’Keefe

¹

,

Tuan

²

,

Lane

³

et al. 2019

Journal of Bone and Mineral Research

View full text Add to dashboard Cite

Cell‐based therapies, defined here as the delivery of cells in vivo to treat disease, have recently gained increasing public attention as a potentially promising approach to restore structure and function to musculoskeletal tissues. Although cell‐based therapy has the potential to improve the treatment of disorders of the musculoskeletal system, there is also the possibility of misuse and misrepresentation of the efficacy of such treatments. The medical literature contains anecdotal reports and research studies, along with web‐based marketing and patient testimonials supporting cell‐based therapy. Both the American Society for Bone and Mineral Research (ASBMR) and the Orthopaedic Research Society (ORS) are committed to ensuring that the potential of cell‐based therapies is realized through rigorous, reproducible, and clinically meaningful scientific discovery. The two organizations convened a multidisciplinary and international Task Force composed of physicians, surgeons, and scientists who are recognized experts in the development and use of cell‐based therapies. The Task Force was charged with defining the state‐of‐the art in cell‐based therapies and identifying the gaps in knowledge and methodologies that should guide the research agenda. The efforts of this Task Force are designed to provide researchers and clinicians with a better understanding of the current state of the science and research needed to advance the study and use of cell‐based therapies for skeletal tissues. The design and implementation of rigorous, thorough protocols will be critical to leveraging these innovative treatments and optimizing clinical and functional patient outcomes. In addition to providing specific recommendations and ethical considerations for preclinical and clinical investigations, this report concludes with an outline to address knowledge gaps in how to determine the cell autonomous and nonautonomous effects of a donor population used for bone regeneration. © 2019 American Society for Bone and Mineral Research.

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“…Nanoparticle‐based treatment of diseases aimed at transferring of drugs to targeted organs and tissues has gained increased attention. EVs have the potential to be used as natural nanoparticles carriers for delivery of therapeutic agents for disease treatment . The imaging and distribution of EVs in vivo are important for the development and optimization of EV‐based therapy, and FCFM has been shown to be suitable for in vivo imaging of polymeric magnetic nanoparticles bearing therapeutic agents within mouse blood vessels and tumors .…”

Section: The Applications Of Fcfm With Cellvizio System In Imaging Anmentioning

confidence: 99%

Shedding light on fibered confocal fluorescence microscopy: Applications in biomedical imaging and therapies

Al-Gubory¹

2019

Journal of Biophotonics

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Discoveries of major importance in life sciences and preclinical research are linked to the invention of microscopes that enable imaging of cells and their microstructures. Imaging technologies involving in vivo procedures using fluorescent dyes that permit labelling of cells have been developed over the last two decades. Fibered confocal fluorescence microscopy (FCFM) is an imaging technology equipped with fiber-optic probes to deliver light to organs and tissues of live animals. This enables not only in vivo detection of fluorescent signals and visualization of cells, but also the study of dynamic processes, such cell proliferation, apoptosis and angiogenesis, under physiological and pathological conditions. This will allow the diagnosis of diseased organs and tissues and the evaluation of the efficacy of new therapies in animal models of human diseases. The aim of this report is to shed light on FCFM and its potential medical applications and discusses some factors that compromise the reliability and reproducibility of monitoring biological processes by FCFM. This report also highlights the issues concerning animal experimentation and welfare, and the contributions of FCFM to the 3Rs principals, replacement, reduction and refinement. K E Y W O R D Sbiomedical imaging, fibered confocal fluorescence microscopy, in vivo cell imaging, therapies

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Exosomes: biology, therapeutic potential, and emerging role in musculoskeletal repair and regeneration

Cited by 44 publications

References 93 publications

American Society for Bone and Mineral Research‐Orthopaedic Research Society Joint Task Force Report on Cell‐Based Therapies – Secondary Publication

American Society for Bone and Mineral Research‐Orthopaedic Research Society Joint Task Force Report on Cell‐Based Therapies – Secondary Publication

American Society for Bone and Mineral Research-Orthopaedic Research Society Joint Task Force Report on Cell-Based Therapies

Shedding light on fibered confocal fluorescence microscopy: Applications in biomedical imaging and therapies

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