Exosomes from C2C12 myoblasts enhance osteogenic differentiation of MC3T3-E1 pre-osteoblasts by delivering miR-27a-3p

Xu, Qian; Cui, Yazhou; Luan, Jing; Zhou, Xiaoyan; Li, Haiying; Han, Jinxiang

doi:10.1016/j.bbrc.2018.02.144

Cited by 81 publications

(64 citation statements)

References 15 publications

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“…With reduced phosphorylation, stabilization and translocation of β-catenin to the nucleus occurs, and nuclear β-catenin then interacts with transcription factors of the Tcf/Lef family to activate specific gene expression programs enhancing osteoblastogenesis and inhibiting osteoclastogenesis (26). Exosomes from myoblasts may promote osteoblastic differentiation by increasing miR-27a-3p levels in recipient preosteoblasts, decreasing expression of APC, and activating the β-catenin pathway (27). Inflammatory cytokines may also activate the Wnt pathway in mineralizing blood vessels, increasing expression of Runx2 (28) and other bone-related genes, such as Rankl, osteocalcin (Bglap), and alkaline phosphatase (Alpl) (29).…”

Section: Introductionmentioning

confidence: 99%

Vitamin D–regulated osteocytic sclerostin and BMP2 modulate uremic extraskeletal calcification

Nguyen-Yamamoto¹,

Tanaka²,

St‐Arnaud³

et al. 2019

JCI Insight

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Section: Introductionmentioning

confidence: 99%

Vitamin D–regulated osteocytic sclerostin and BMP2 modulate uremic extraskeletal calcification

Nguyen-Yamamoto¹,

Tanaka²,

St‐Arnaud³

et al. 2019

JCI Insight

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“…Osteoblast-derived exosomes and pre-osteoblasts-derived cells can also carry miRNAs such as let-7a and miR-96a, which have been previously confirmed to be involved in bone remodeling [79]. Similarly, the miR-27a-3p carried by myogenic cell-derived exosomes can also enhance osteogenic differentiation of pre-osteoblasts [80]. In contrast, osteoclast-derived exosomes can carry miRNAs such as miR-214 that inhibit osteogenic differentiation of osteoblasts [81].…”

Section: Genetic Materials Carried By Exosomes Regulate Bone Regeneramentioning

confidence: 98%

Potential Therapeutic Applications of Exosomes in Bone Regenerative Medicine

Yang¹,

Zhu²,

Lu³

et al. 2019

Osteogenesis and Bone Regeneration

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The ability of bone regeneration is relatively robust, which is crucial for fracture healing, but delayed healing and nonunion are still common problems in clinical practice. Fortunately, exciting results have been achieved for regenerative medicine in recent years, especially in the area of stem cell-based treatment, but all these cell-based approaches face challenging problems, including immune rejection. For this reason, exosomes, stem cell-derived small vesicles of endocytic origin, have attracted the attention of many investigators in the field of bone regeneration. One of the attractive features of exosomes is that they are small and can travel between cells and deliver bioactive products, including miRNA, mRNA, proteins, and various other factors, to promote bone regeneration, with undetectable immune rejection. In this chapter, we intend to briefly update the recent progressions, and discuss the potential challenges in the target areas. Hopefully, our discussion would be helpful not only for the clinicians and the researchers in the specific disciplines but also for the general audiences as well.

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“…When miR-27a-3p was depleted, targeted MC3T3-E1 cell exosome-dependent differentiation was prevented. A β-catenin dependent mechanism mediated this exosome (+miR-27a-3p) action in osteoblasts (Xu et al, 2018). MSC exosomes carry miRNAs that impact osteoblast physiology and bone regeneration.…”

Section: Msc Exosome Roles In Oral and Craniofacial Tissue Engineeringmentioning

confidence: 99%

A Role for Exosomes in Craniofacial Tissue Engineering and Regeneration

et al. 2020

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Tissue engineering and regenerative medicine utilize mesenchymal stem cells (MSCs) and their secretome in efforts to create or induce functional tissue replacement. Exosomes are specific extracellular vesicles (EVs) secreted by MSCs and other cells that carry informative cargo from the MSC to targeted cells that influence fundamental cellular processes including apoptosis, proliferation, migration, and lineage-specific differentiation. In this report, we review the current knowledge regarding MSC exosome biogenesis, cargo and function. This review summarizes the use of MSC exosomes to control or induce bone, cartilage, dentin, mucosa, and pulp tissue formation. The next-step engineering of exosomes provides additional avenues to enhance oral and craniofacial tissue engineering and regeneration.

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Exosomes from C2C12 myoblasts enhance osteogenic differentiation of MC3T3-E1 pre-osteoblasts by delivering miR-27a-3p

Cited by 81 publications

References 15 publications

Vitamin D–regulated osteocytic sclerostin and BMP2 modulate uremic extraskeletal calcification

Vitamin D–regulated osteocytic sclerostin and BMP2 modulate uremic extraskeletal calcification

Potential Therapeutic Applications of Exosomes in Bone Regenerative Medicine

A Role for Exosomes in Craniofacial Tissue Engineering and Regeneration

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