Exosomes from SIRT1-Overexpressing ADSCs Restore Cardiac Function by Improving Angiogenic Function of EPCs

Huang, Hui; Xu, Zhengfu; Qi, Yuan; Zhang, Wei; Zhang, Chenjun; Jiang, Mei; Deng, Shengqiong; Wang, Hairong

doi:10.1016/j.omtn.2020.07.007

Cited by 45 publications

(27 citation statements)

References 59 publications

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“…ASCs EVs could promote angiogenesis mainly via transferring functional microRNAs to targeted cells [ 19 , 56 , 85 , 103 , 122 , 168 , 169 , 173 , 177 , 180 , 181 ]. The underlying mechanisms for angiogenesis potential of ASCs EVs were shown as follows.…”

Section: Resultsmentioning

confidence: 99%

Adipose stem cells-released extracellular vesicles as a next-generation cargo delivery vehicles: a survey of minimal information implementation, mass production and functional modification

et al. 2022

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Objectives To investigate current situation of minimal information implementation highlighted by minimal information for studies of extracellular vesicles 2018 (MISEV2018) guidelines, and explore technological advances towards mass production and functional modification in aesthetic, plastic and reconstructive surgery. Methods Original articles on extracellular vesicles (EVs) of adipose stem cells (ASCs) were identified. Statistics upon minimal information for EVs research, such as species, cell types, culture conditions, conditioned media harvesting parameters, EVs isolation/storage/identification/quantification, functional uptake and working concentration, were analyzed. Results The items of cell culture conditions such as passage number, seeding density, conditioned media harvesting time, functional uptake and working concentration were poorly documented, with a reporting percentage of 47.13%, 54.02%, 29.89%, 62.07% and 36.21%, respectively. However, there were some studies not reporting information of ASCs origin, culture medium, serum, EVs isolation methods, quantification and identification of EVs, accounting for 3.45%, 10.34%, 6.90%, 3.45%, 18.39% and 4.02%, respectively. Serum deprivation and trophic factors stimuli were attempted for EVs mass production. Several technological advances towards functional modification included hypoxia pre-condition, engineering EVs and controlled release. Presently, ASCs EVs have been applied in multiple fields, including diabetic/non-diabetic wound healing, angiogenesis, inflammation modulation, fat grafting, hair regeneration, antiaging, and healing and regeneration of cartilage/bone/peripheral nerve/tendon. Conclusion Our results highlight normative reporting of ASCs EVs in functional studies to increase reliability and reproducibility of scientific publications. The advances towards mass production and functional modification of ASCs EVs are also recommended to enhance therapeutic effects.

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Section: Resultsmentioning

confidence: 99%

Adipose stem cells-released extracellular vesicles as a next-generation cargo delivery vehicles: a survey of minimal information implementation, mass production and functional modification

et al. 2022

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“…miR-21 in EnMSC-derived exosomes showed superior cardioprotection through angiogenic effects via the PTEN/Akt pathway [83]. Additionally, the exosomes from SIRT1-overexpressing ADSCs can help to restore the function of cell migration and tube formation and recruitment of EPCs to the repair area through the Nrf2/CXCL12/CXCR7 pathway [84]. Macrophage migration inhibitory factor engineered hUC-MSC-produced exosomes also significantly enhanced proliferation, migration, and angiogenesis by delivering miR-133a-3p to HUVEC [85].…”

Section: Myocardial Preservationmentioning

confidence: 99%

The Application Potential and Advance of Mesenchymal Stem Cell-Derived Exosomes in Myocardial Infarction

Wang

Tang

Liu

et al. 2021

Stem Cells International

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Myocardial infarction (MI) is a devastating disease with high morbidity and mortality caused by the irreversible loss of functional cardiomyocytes and heart failure (HF) due to the restricted blood supply. Mesenchymal stem cells (MSCs) have been emerging as lead candidates to treat MI and subsequent HF mainly through secreting multitudinous factors of which exosomes act as the most effective constituent to boost the repair of heart function through carrying noncoding RNAs and proteins. Given the advantages of higher stability in the circulation, lower toxicity, and controllable transplantation dosage, exosomes have been described as a wonderful and promising cell-free treatment method in cardiovascular disease. Nowadays, MSC-derived exosomes have been proposed as a promising therapeutic approach to improve cardiac function and reverse heart remodeling. However, exosomes’ lack of modification cannot result in desired therapeutic effect. Hence, optimized exosomes can be developed via various engineering methods such as pharmacological compound preconditioned MSCs, genetically modified MSCs, or miRNA-loaded exosomes and peptide tagged exosomes to improve the targeting and therapeutic effects of exosomes. The biological characteristics, therapeutic potential, and optimizing strategy of exosomes will be described in our review.

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“…130 More recently, Huang et al applied exosomes derived from genetically modified ASCs to restore cardiac function through improving the angiogenic function of peripheral blood EPCs. 131 Indeed, the authors aimed to understand whether exosomes derived from silencing information regulator factor 1 (SIRT1) overexpressing ASCS (ASCs-SIRT1-Exos) may promote angiogenesis and myocardial repair of acute MI (AMI). The obtained results indicated that AMI could downregulate CXCR7 expression in EPCs and reduced their angiogenic function.…”

Section: Adipose-derived Stem Cellsmentioning

confidence: 99%

Stem cell‐based therapies for cardiac diseases: The critical role of angiogenic exosomes

et al. 2021

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Finding effective treatments for cardiac diseases is among the hottest subjects in medicine; cell-based therapies have brought great promises for managing a broad range of life-threatening heart complications such as myocardial infarction. After clarifying the critical role of angiogenesis in tissue repair and regeneration, various stem/progenitor cell were utilized to accelerate the healing of injured cardiac tissue. Embryonic, fetal, adult, and induced pluripotent stem cells have shown the appropriate proangiogenic potential for tissue repair strategies. The capability of stem cells for differentiating into endothelial lineages was initially introduced as the primary mechanism involved in improving angiogenesis and accelerated heart tissue repair. However, recent studies have demonstrated the leading role of paracrine factors secreted by stem cells in advancing neo-vessel formation. Genetically modified stem cells are also being applied for promoting angiogenesis regarding their ability to considerably overexpress and secrete angiogenic bioactive molecules. Yet, conducting further research seems necessary to precisely identify molecular mechanisms behind the proangiogenic potential of stem cells, including the signaling pathways and regulatory molecules such as microRNAs. In conclusion, stem cells' pivotal roles in promoting angiogenesis and consequent improved cardiac healing and remodeling processes should not be ignored, especially in the case of stem cellderived extracellular vesicles.

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Exosomes from SIRT1-Overexpressing ADSCs Restore Cardiac Function by Improving Angiogenic Function of EPCs

Cited by 45 publications

References 59 publications

Adipose stem cells-released extracellular vesicles as a next-generation cargo delivery vehicles: a survey of minimal information implementation, mass production and functional modification

Adipose stem cells-released extracellular vesicles as a next-generation cargo delivery vehicles: a survey of minimal information implementation, mass production and functional modification

The Application Potential and Advance of Mesenchymal Stem Cell-Derived Exosomes in Myocardial Infarction

Stem cell‐based therapies for cardiac diseases: The critical role of angiogenic exosomes

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