Exosomes Function in Pro- and Anti-Angiogenesis

Ribeiro, Mara Fernandes; Zhu, Hongyan; Millard, Ronald W.; Gao, Fan

doi:10.2174/22115528113020020001

Cited by 147 publications

(37 citation statements)

References 57 publications

(63 reference statements)

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“…New blood vessel formation, which involves endothelial cell proliferation, migration and branching to form capillaries, requires a dynamic regulated interaction between endothelial cells, angiogenesis factors and the surrounding extracellular matrix (45). It is known that some exosomes can regulate the angiogenic function of recipient endothelial cells by transferring exosomal proteins, RNAs and miRNAs into the cytoplasm of these cells (46). Such exosomes represent a highly attractive delivery vehicle for any proteins or RNAs through which they can exert their therapeutic effects.…”

Section: Discussionmentioning

confidence: 99%

Exosomes derived from dental pulp stem cells accelerate cutaneous wound healing by enhancing angiogenesis via the Cdc42/p38 MAPK pathway

et al. 2022

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Skin wound healing is a common challenging clinical issue which requires advanced treatment strategies. The present study investigated the therapeutic effects of exosomes derived from dental pulp stem cells (DPSC-Exos) on cutaneous wound healing and the underlying mechanisms. The effects of DPSC-Exos on cutaneous wound healing in mice were examined by measuring wound closure rates, and using histological and immunohistochemical analysis. A series of functional assays were performed to evaluate the effects of DPSC-Exos on the angiogenic activities of human umbilical vein endothelial cells (HUVECs) in vitro. Tandem mass tag-based quantitative proteomics analysis of DPSCs and DPSC-Exos was performed. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were used to evaluate the biological functions and pathways for the differentially expressed proteins in DPSC-Exos. Western blot analysis was used to assess the protein levels of cell division control protein 42 (Cdc42) and p38 in DPSC-Exos and in HUVECs subjected to DPSC-Exos-induced angiogenesis. SB203580, a p38 mitogen-activated protein kinase (MAPK) signaling pathway inhibitor, was employed to verify the role of the p38 MAPK pathway in vitro and in vivo. Histological and immunohistochemical staining revealed that the DPSC-Exos accelerated wound healing by promoting neovascularization. The DPSC-Exos promoted the migration, proliferation and capillary formation capacity of HUVECs. Proteomics data demonstrated that proteins contained in DPSC-Exos regulated vasculature development and angiogenesis. Pathway analysis revealed that proteins expressed in DPSC-Exos were involved in several pathways, including MAPK pathway. Western blot analysis demonstrated that the DPSC-Exos increased the protein levels of Cdc42 and phosphorylation of p38 in HUVECs. SB203580 suppressed the angiogenesis induced by DPSC-Exos. On the whole, the present study demonstrates that DPSC-Exos accelerate cutaneous wound healing by enhancing the angiogenic properties of HUVECs via the Cdc42/p38 MAPK signaling pathway.

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Section: Discussionmentioning

confidence: 99%

Exosomes derived from dental pulp stem cells accelerate cutaneous wound healing by enhancing angiogenesis via the Cdc42/p38 MAPK pathway

et al. 2022

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“…Exosomes (30-100 nm in diameter) are smaller than PMVs and are derived from endosomal multivesicular bodies and α-granules. 23 Interestingly, activated platelets also release respiratory-competent mitochondria as free organelles that can act as damage-associated molecular patterns in several pathologies. 24 …”

Section: Functions Of Platelets: Beyond Hemostasismentioning

confidence: 99%

The multifaceted role of platelets in mediating brain function

Burnouf

Walker

2022

Blood

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Platelets, the small, anucleate blood cells that originate from megakaryocytes in the bone marrow, are typically associated with coagulation. However, it is now apparent that platelets are more multifaceted than originally thought, with their function extending beyond their traditional role in hemostasis to acting as important mediators of brain function. In this review, we outline the broad repertoire of platelet function in the central nervous system, focusing on the similarities between platelets and neurons. We also summarize the role that platelets play in the pathophysiology of various neurological diseases, with a particular focus on neuroinflammation and neurodegeneration. Finally, we highlight the exciting prospect of harnessing the unique features of the platelet proteome and extracellular vesicles, which are rich in neurotrophic, antioxidative, and antiinflammatory factors, for the development of novel neuroprotective and neuroregenerative interventions to treat various neurodegenerative and traumatic pathologies.

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“…favouring intercellular communications (55, 57) (Figure 1), participating in cell homeostasis (58), stimulating immune responses (59), promoting tissue repairing (60), cell survival (61), and modulating angiogenesis (62). For these reasons, they are considered as crucial modulators of intercellular cross-talks at the base of several high-impact diseases (63-65), including cancer.…”

Section: Exosomesmentioning

confidence: 99%

Role of exosomal microRNAs in cancer therapy and drug resistance mechanisms: focus on hepatocellular carcinoma

Zelli

Compagnoni²,

Capelli³

et al. 2022

Front. Oncol.

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Extracellular vesicles (EVs), defined as intercellular messengers that carry their cargos between cells, are involved in several physiological and pathological processes. These small membranous vesicles are released by most cells and contain biological molecules, including nucleic acids, proteins and lipids, which can modulate signaling pathways of nearby or distant recipient cells. Exosomes, one the most characterized classes of EVs, include, among others, microRNAs (miRNAs), small non-coding RNAs able to regulate the expression of several genes at post-transcriptional level. In cancer, exosomal miRNAs have been shown to influence tumor behavior and reshape tumor microenvironment. Furthermore, their possible involvement in drug resistance mechanisms has become evident in recent years. Hepatocellular carcinoma (HCC) is the major type of liver cancer, accounting for 75-85% of all liver tumors. Although the improvement in HCC treatment approaches, low therapeutic efficacy in patients with intermediate-advanced HCC is mainly related to the development of tumor metastases, high risk of recurrence and drug resistance. Exosomes have been shown to be involved in pathogenesis and progression of HCC, as well as in drug resistance, by regulating processes such as cell proliferation, epithelial-mesenchymal transition and immune response. Herein, we summarize the current knowledge about the involvement of exosomal miRNAs in HCC therapy, highlighting their role as modulators of therapeutic response, particularly chemotherapy and immunotherapy, as well as possible therapeutic tools.

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Exosomes Function in Pro- and Anti-Angiogenesis

Cited by 147 publications

References 57 publications

Exosomes derived from dental pulp stem cells accelerate cutaneous wound healing by enhancing angiogenesis via the Cdc42/p38 MAPK pathway

Exosomes derived from dental pulp stem cells accelerate cutaneous wound healing by enhancing angiogenesis via the Cdc42/p38 MAPK pathway

The multifaceted role of platelets in mediating brain function

Role of exosomal microRNAs in cancer therapy and drug resistance mechanisms: focus on hepatocellular carcinoma

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