Exosomes in Human Breast Milk Promote EMT

Qin, Wenyi; Tsukasaki, Yoshikazu; Dasgupta, Santanu; Mukhopadhyay, Nitai D.; Ikebe, Mitsuo; Sauter, Edward R.

doi:10.1158/1078-0432.ccr-16-0135

Cited by 122 publications

(97 citation statements)

References 30 publications

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“…According to more than 30 studies, from different teams around the world, milk EVs resisted in vitro digestion (Benmoussa et al., ; Kahn et al., ; Liao et al., ; Rani et al., ; Shandilya et al., ; Vashisht et al., ) were internalized by recipient cells upon co‐incubation (Admyre et al., , 2008; Aqil et al., , ; Badawy et al., ; Golan‐Gerstl et al., ; Hock et al., ; Kahn et al., ; Liao et al., ; Maburutse, Park, Oh, & Kim, ; Martin et al., ; Matsuda & Patel, ; Mobley et al., ; Munagala et al., ; Naslund et al., ; Qin et al., ; Vashisht, Rani, Onteru, & Singh, ; Yu et al., ), transduced through the intestinal wall in vitro (Rani et al., ; Shandilya et al., ; Wolf et al., ), and were detected in animal models after ingestion (Manca et al., ; Munagala et al., ) or intravenous injection (Somiya, Yoshioka, & Ochiya, ).…”

Section: Milk Micrornas In Health and Diseasementioning

confidence: 99%

“…Twenty‐one studies involving milk EVs from humans, rat, camels, and most often cow milks reported milk EVs bioactivity in vitro on cells (Admyre et al., ; Aqil et al., , ; Badawy et al., ; Golan‐Gerstl et al., ; Hock et al., ; Martin et al., ; Matsuda & Patel, ; Mobley et al., ; Munagala et al., ; Naslund et al., ; Qin et al., ; Vashisht et al., ; Yu et al., ) and in vivo in animal models (Agrawal et al., ; Aqil et al., , ; Badawy et al., ; Leiferman et al., ; Munagala et al., ; Munagala et al., ; Somiya et al., ).…”

Section: Milk Micrornas In Health and Diseasementioning

confidence: 99%

“…Functionality of milk EVs has been explored following their intrinsic biological activity or their potential as drug carriers for cancer treatment (Agrawal et al., ; Aqil et al., , ; Badawy et al., ; Munagala et al., , ; Qin et al., ; Somiya et al., ), for the modulation of inflammation (Admyre et al., ; Arntz et al., ; Badawy et al., ; Martin et al., ; Vashisht et al., ), for gut, bone, and muscle development (Chen et al., ; Hock et al., ; Leiferman et al., ; Martin et al., ; Mobley et al., ; Oliveira et al., , ), bacterial proliferation (Yu et al., ), and in the prevention of HIV infection (Naslund et al., ).…”

Section: Milk Micrornas In Health and Diseasementioning

confidence: 99%

“…Recent in vitro reports suggest that milk EVs promote epithelial‐to‐mesenchymatous transition (EMT; Qin et al., ), whereas others have reported evidence that milk EVs inhibit the proliferation of cancerous cells; especially in breast, ovarian, and lung cancers (Aqil et al., , ) and sensitize cancer cells to chemotherapy (Agrawal et al., ; Aqil et al., , , ). These results came often with in vivo validation studies confirming the tumor‐suppressant activity of milk EVs, in various murine and rat cancer models (Agrawal et al., ; Aqil et al., , ; Badawy et al., ; Munagala et al., , ; Somiya et al., ).…”

Section: Milk Micrornas In Health and Diseasementioning

confidence: 99%

See 3 more Smart Citations

Milk MicroRNAs in Health and Disease

Benmoussa

Provost

2019

Comp Rev Food Sci Food Safe

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MicroRNAs are small noncoding RNAs responsible for regulating 40% to 60% of gene expression at the posttranscriptional level. The discovery of circulating microRNAs in several biological fluids opened the path for their study as biomarkers and long‐range cell‐to‐cell communication mediators. Their transfer between individuals in the case of blood transfusion, for example, and their high enrichment in milk have sparked the interest for microRNA transfer through diet, especially from mothers to infants during breastfeeding. The extension of such paradigm led to the study of milk microRNAs in the case of cow or goat milk consumption in adults. Here we provide a comprehensive critical review of the key findings surrounding milk microRNAs in human, cow, and goat milk among other species. We discuss the data on their biological properties, their use as disease biomarkers, their transfer between individuals or species, and their putative or verified functions in health and disease of infants and adult consumers. This work is based on all the literature available and integrates all the results, theories, debates, and validation studies available so far on milk microRNAs and related areas of investigations. We critically discuss the limitations and outline future aspects and avenues to explore in this rapidly growing field of research that could impact public health through infant milk formulations or new therapies. We hope that this comprehensive review of the literature will provide insight for all teams investigating milk RNAs’ biological activities and help ensure the quality of future reports.

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Section: Milk Micrornas In Health and Diseasementioning

confidence: 99%

Section: Milk Micrornas In Health and Diseasementioning

confidence: 99%

Section: Milk Micrornas In Health and Diseasementioning

confidence: 99%

Section: Milk Micrornas In Health and Diseasementioning

confidence: 99%

See 2 more Smart Citations

Milk MicroRNAs in Health and Disease

Benmoussa

Provost

2019

Comp Rev Food Sci Food Safe

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“…Reduced CK8 expression is regarded as an indicator of EMT, leading to more malignant forms of cancer [89]. Breast milk exosomes containing high levels of TGF-β2 can induce changes in both benign and malignant breast epithelial cells, consistent with the development and progression of breast cancer, which occurs due to alterations in cellular shape and the actin cytoskeleton and the loss of cell-cell junctions [90]. TGF-β-induced EMT was found to restrain cell invasion, which may be alleviated by the overexpression of hyperactivated Ras [91].…”

Section: The Role Of the Cellular Cytoskeleton In Epithelial-mesenchymentioning

confidence: 99%

Novel Insights into the Role of the Cytoskeleton in Cancer

Zhang¹,

Pei²,

Ji³

et al. 2017

Cytoskeleton - Structure, Dynamics, Function and Disease

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The cytoskeleton is a complex network of highly ordered intracellular ilaments that plays a central role in controlling cell shape, division, functions, and interactions in human organs and tissues, but dysregulation of this network can contribute to numerous human diseases, including cancer. To clarify the various functions of the cytoskeleton and its role in cancer progression, in this chapter, we will discuss the microilament (actin cytoskeleton), microtubule (β-tubulin), and intermediate ilament (keratins, cytokeratins, vimentin, and lamins) cytoskeletal structures; analyze the physiological functions of the cytoskeleton and its regulation of cell diferentiation; and investigate the roles of the cytoskeleton in cancer progression, the epithelial-mesenchymal transition process (EMT), and the mechanisms of multidrug resistance (MDR) in relation to the cytoskeleton. Importantly, the cytoskeleton, as a key regulator of the transcription and expression of many genes, is known to be involved in various physiological and pathological processes, which makes the cytoskeleton a novel and highly efective target for assessing the response to antitumor therapy in cancer.Keywords: Cytoskeleton, Regulator, cell diferentiation, drug resistance, EMT IntroductionThe cytoskeleton is a structure similar to a bird's nest; it can be tightly packed or sparse and is found in both prokaryotes and eukaryotes [1]. The main component of the cytoskeleton is protein, and the speciic diferences in structure never afect the type of proteins incorporated [2]. The cytoskeletons of prokaryotes display apparent plasticity in composition, without conservation of the core ilament-forming proteins. However, the eukaryotic cytoskeleton has evolved in a variety of functions through the addition of accessory proteins and extensive There are three main cytoskeletal structures in eukaryotes, microilaments (MFs, ≈7 nm diameter), microtubules (MTs, ≈25 nm diameter), and intermediate ilaments (IFs, ≈10 nm diameter) [1]. MFs are responsible for cell contraction and reinforcement of the cell surface and allow changes in cell morphology. Actin and tubulin are the main globular proteins that form MFs and MTs, respectively. Actin is a ubiquitous eukaryotic ilament-forming protein. Actin ilaments (also called microilaments or F-actin) consist of two proto ilament polymers wound together in a right-handed helix [3]. Eukaryotic actin is a member of a large and diverse superfamily of ATPases that includes Hsp70 chaperones and several classes of sugar/sugar alcohol kinases [4,5] as well as eukaryotic actin-related proteins (ARPs) [6,7]. The actin cytoskeleton is involved in actin-based cytoskeletal structures, including various functionally distinct structures of actin organization, and can be regulated by actin regulatory proteins. It is well known that the actin cortex consists of a dense mesh-like array of F-actin anchored to the cell membranes [8,9]. This structure provides the core "skeleton" of the cell, functioning to deine cell shape and provi...

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Exosomal miRNAs as biomarkers for diagnostic and prognostic in lung cancer

Shen

2020

Cancer Medicine

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According to the 2018 global cancer statistics, lung cancer is the cancer with the highest morbidity (1.6 percent of total cases) and mortality (18.4 percent of total cancer deaths) in the world. 1 Unfortunately, despite the decline in lung cancer mortality, the majority of patients are still diagnosed with advanced or metastatic lung cancer, leading to poor outcomes. 2 Therefore, detection of lung cancer in the early stage before clinical symptoms, which may be an effective means to reduce cancer mortality. So it is worth our efforts to find effective and reliable biomarkers. Despite advances in the treatment of lung cancer, the prognosis of patients is not satisfactory. 3 At present, we urgently need to find biomarkers that may predict the recurrence of lung cancer after surgery to improve the prognosis of patients. Exosomes are vesicles with a diameter of 40-100 nm. They sprout to form early multivesicular bodies (MVB). When fused with the plasma membrane, they form an intracellular vesicle (ILV), which is released into the extracellular environment (Figure 1). 4,5 It is noteworthy that most cells can secrete exosomes. Therefore, exosomes are widely present in a variety of biological fluids, such as semen, 6 urine, 7 serum, 8 plasma, 9 saliva, 10 bile, 11 breast milk, 12 amniotic fluid, 13 cerebrospinal

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Exosomes in Human Breast Milk Promote EMT

Cited by 122 publications

References 30 publications

Milk MicroRNAs in Health and Disease

Milk MicroRNAs in Health and Disease

Novel Insights into the Role of the Cytoskeleton in Cancer

Exosomal miRNAs as biomarkers for diagnostic and prognostic in lung cancer

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