Exosomes‐Loaded Electroconductive Hydrogel Synergistically Promotes Tissue Repair after Spinal Cord Injury via Immunoregulation and Enhancement of Myelinated Axon Growth

Fan, Lei; Liu, Can; Chen, Xiuxing; Zheng, Lei; Zou, Yan; Wen, Huiquan; Guan, Pengfei; Lu, Fang; Luo, Yian; Tan, Guoxin; Yu, Peng; Chen, Dafu; Deng, Chunlin; Sun, Yongjian; Lei, Zhou; Ning, Chengyun

doi:10.1002/advs.202105586

Cited by 202 publications

(172 citation statements)

References 61 publications

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“…In brief, it turned out that GMN group effectively promoted the connection between the newborn nerves with the injured nerve. Lei et al has proved that the electroconductive hydrogel with bone marrow stem cell-derived exosomes can improve the microenvironment of the injured site [ 41 ]. It showed that electroconductive hydrogel also need the good biocompatibility.…”

Section: Resultsmentioning

confidence: 99%

GelMA-MXene hydrogel nerve conduits with microgrooves for spinal cord injury repair

Cai

Zhang

et al. 2022

J Nanobiotechnol

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Repair of spinal cord injury (SCI) depends on microenvironment improvement and the reconnection between injured axons and regenerated neurons. Here, we fabricate a GelMA-MXene hydrogel nerve conduit with electrical conductivity and internal-facing longitudinal grooves and explore its function in SCI repair. It is found that the resultant grooved GelMA-MXene hydrogel could effectively promote the neural stem cells (NSCs) adhesion, directed proliferation and differentiation in vitro. Additionally, when the GelMA-MXene conduit loaded with NSCs (GMN) is implanted into the injured spinal cord site, effective repair capability for the complete transection of SCI was demonstrated. The GMN group shows remarkable nerve recovery and significantly higher BBB scores in comparison to the other groups. Therefore, GMN with the microgroove structure and loaded with NSCs is a promising strategy in treating SCI.

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Section: Resultsmentioning

confidence: 99%

GelMA-MXene hydrogel nerve conduits with microgrooves for spinal cord injury repair

Cai

Zhang

et al. 2022

J Nanobiotechnol

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“…BMSCs (BMSCs-Exos). BMSCs were collected by fushing the bone marrow cavity of 2-week-old rats' tibia and femur, as previously described [19]. Te isolated BMSCs were put in a low-glucose DMEM (Gibco) mixture containing 10% serum and cultured at 37 °C with 5% CO 2 in a cell culture incubator.…”

Section: Isolation Identifcation and Staining Of Exosomes Frommentioning

confidence: 99%

ECM-Mimicking Hydrogels Loaded with Bone Mesenchymal Stem Cell-Derived Exosomes for the Treatment of Cartilage Defects

Cheng

Rong

Wang

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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It is well-established that treating articular cartilage injuries is clinically challenging since they lack blood arteries, nerves, and lymphoid tissue. Recent studies have revealed that bone marrow stem cell-derived exosomes (BMSCs-Exos) exert significant chondroprotective effects through paracrine secretions, and hydrogel-based materials can synergize the exosomes through sustained release. Therefore, this research aims to synthesize an ECM (extracellular matrix)-mimicking gelatin methacryloyl (GelMA) hydrogel modified by gelatin combined with BMSCs-derived exosomes to repair cartilage damage. We first isolated and characterized exosomes from BMSCs supernatant and then loaded the exosomes into GelMA hydrogel to investigate cartilage repair effects in in vitro and in vivo experiments. The outcomes showed that the GelMA hydrogel has good biocompatibility with a 3D (three-dimensional) porous structure, exhibiting good carrier characteristics for exosomes. Furthermore, BMSCs-Exos had a significant effect on promoting chondrocyte ECM production and chondrocyte proliferation, and the GelMA hydrogel could enhance this effect through a sustained-release effect. Similarly, in vivo experiments showed that GelMA-Exos promoted cartilage regeneration in rat joint defects and the synthesis of related cartilage matrix proteins.

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“…The larger the radius, the larger the charge transfer resistance. 70 The introduction of Cu 2+ and PANI enhanced the conductivity of hydrogels.…”

Section: Resultsmentioning

confidence: 99%

Mimic enzymatic preparation of conductive supramolecular-polymeric hydrogels with antibacterial and antioxidant properties for accelerating wound healing

et al. 2023

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Exosomes‐Loaded Electroconductive Hydrogel Synergistically Promotes Tissue Repair after Spinal Cord Injury via Immunoregulation and Enhancement of Myelinated Axon Growth

Cited by 202 publications

References 61 publications

GelMA-MXene hydrogel nerve conduits with microgrooves for spinal cord injury repair

GelMA-MXene hydrogel nerve conduits with microgrooves for spinal cord injury repair

ECM-Mimicking Hydrogels Loaded with Bone Mesenchymal Stem Cell-Derived Exosomes for the Treatment of Cartilage Defects

Mimic enzymatic preparation of conductive supramolecular-polymeric hydrogels with antibacterial and antioxidant properties for accelerating wound healing

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