Exosomes released by granulocytic myeloid-derived suppressor cells attenuate DSS-induced colitis in mice

Abstract

Myeloid-derived suppressor cells (MDSC) have been described in inflammatory bowel disease (IBD), but their role in the disease remains controversial. We sought to define the effect of granulocytic MDSC-derived exosomes (G-MDSC exo) in dextran sulphate sodium (DSS)-induced murine colitis. G-MDSC exo-treated mice showed greater resistance to colitis, as reflected by lower disease activity index, decreased inflammatory cell infiltration damage. There was a decrease in the proportion of Th1 cells and an increase in the proportion of regulatory T cells (Tregs) in mesenteric lymph nodes (MLNs) from G-MDSC exo-treated colitis mice. Moreover, lower serum levels of interferon (IFN)-γ and tumor necrosis factor (TNF)-α were detected in G-MDSC exo-treated colitis mice. Interestingly, inhibition of arginase (Arg)-1 activity in G-MDSC exo partially abrogated the spontaneous improvement of colitis. In addition, G-MDSC exo could suppress CD4+ T cell proliferation and IFN-γ secretion in vitro and inhibit the delayed-type hypersensitivity (DTH) response, and these abilities were associated with Arg-1 activity. Moreover, G-MDSC exo promoted the expansion of Tregs in vitro. Taken together, these results suggest that G-MDSC exo attenuate DSS-induced colitis through inhibiting Th1 cells proliferation and promoting Tregs expansion.

Atorvastatin promotes the expansion of myeloid‐derived suppressor cells and attenuates murine colitis

“…Previous studies have demonstrated that statins have therapeutic benefits for the treatment in animal models of autoimmune disease, including inflammatory bowel disease . Intriguingly, a number of studies have shown beneficial effects of MDSCs in treating inflammatory bowel disease (IBD) . We therefore speculated that atorvastatin‐derived G‐MDSCs may reduce the severity of murine experimental colitis.…”

Granulocytic Myeloid‐Derived Suppressor Cells Promote the Stemness of Colorectal Cancer Cells through Exosomal S100A9

“…[20] Cells were removed from culture supernatants by centrifugation at 500 g for 5 min. [20] Cells were removed from culture supernatants by centrifugation at 500 g for 5 min.…”

“…[16] Researchers have elucidated the complex roles of exosomes in cancer progression. [20] These results drove us to further observe whether MDSCs communicate with CRC cells via exosomes secretion. [19] Especially, S100A9 was abundant in MDSC-derived exosomes.…”

CD4+ T Cell‐Released Extracellular Vesicles Potentiate the Efficacy of the HBsAg Vaccine by Enhancing B Cell Responses

“…[11] Briefly, cells and cellular debris were removed by sequential centrifugation at 300 × g for 20 min, 1000 × g for 30 min, and 10 000 × g for 30 min. The culture supernatant of CD4 + T cells was harvested.…”