Exosomes secreted by hypoxia-stimulated bone-marrow mesenchymal stem cells promote grafted tendon-bone tunnel healing in rat anterior cruciate ligament reconstruction model

Zhang, Tao; Yan, Shaohang; Ye, Song; Chen, Can; Xu, Daqi; Lu, Bin; Xu, Yan

doi:10.1016/j.jot.2022.08.001

Cited by 23 publications

(39 citation statements)

References 52 publications

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“…[55] Furthermore, Zhang et al proved that sEVs secreted by hypoxia-preconditioned BM MSCs could accelerate grafted tendon-bone tunnel healing by coupling with peri-graft bone and blood vessel microarchitecture. [56] Finally, Cai et al showed that sEVs derived from Kartogenin-stimulated BM MSCs, when loaded in a sodium alginate HG, could promote cartilage formation and collagen maturation for enthesis regeneration in chronic RCT. [57]…”

Section: Muscle and Tendon Tearmentioning

confidence: 99%

Biomaterial‐Facilitated Local Delivery of Stem Cell‐Derived Small Extracellular Vesicles: Perspectives in Surgical Therapy

Tan,

Xu,

et al. 2024

Advanced Therapeutics

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Stem cell‐derived small extracellular vesicles that are either naturally produced or pharmacogenetically engineered have shown tremendous potential in regenerative medicine in recent years. These sEVs exert multiple therapeutic effects comparable or superior to their parental stem cells while avoiding the limitations of stem cell‐based therapy, such as infusion toxicity, immunogenicity, tumorigenic potential, and ethical issues. The two routine modalities of sEV administration are systemic injection and local delivery, each presenting distinct benefits and drawbacks. Various biomaterials have been developed to assist their local delivery to improve the targeting of organs, minimize non‐specific accumulation in vital organs, and ensure the protection and release of sEVs. Based on a thorough search and meticulous dissection of relevant literature from the past five years, we present this comprehensive, up‐to‐date, specialty‐specific, disease‐oriented, and preclinical review to expound on the surgical application and potential of stem cell‐derived sEVs. The local delivery of stem cell‐derived sEVs, which possess similar indications as systemic transfer, could treat numerous diseases encountered in orthopedic surgery, neurosurgery, plastic surgery, cardiothoracic surgery, general surgery, urology, otorhinolaryngology, ophthalmology, obstetrics and gynecology, and dental surgery. In addition, the biomaterials utilized encompass a wide range of sources (e.g., natural, synthetic, and hybrid polymers), format (e.g., scaffold, patch, spray, microneedle), and responsiveness (e.g., temperature, pH, and protein), which enables customized sEV therapy tailored to specific diseases. This review demonstrates biomaterial‐facilitated local delivery of stem cell‐derived sEVs as a viable alternative or even a superior option to systemic sEV therapy. Future collaboration among surgeons, biomaterial scientists, and stem cell researchers is essential to advance this research area.This article is protected by copyright. All rights reserved

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Section: Muscle and Tendon Tearmentioning

confidence: 99%

Biomaterial‐Facilitated Local Delivery of Stem Cell‐Derived Small Extracellular Vesicles: Perspectives in Surgical Therapy

Tan,

Xu,

et al. 2024

Advanced Therapeutics

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“…Since the introduction and widespread application of cell-free therapies based on exosomes, extracellular vesicles derived from ADSCs, BMSCs, and urine-derived stem cells (USCs) have been proven to promote tendon repair by modulating the biological characteristics of TSCs and the extracellular microenvironment. Preconditioning such stem cells under hypoxic conditions has shown to be advantageous in promoting vascular generation and accelerating tendon-to-bone healing [ 235 , 296 , 297 ]. In another study, extracellular vesicle products derived from human platelets expressing CD41a and CD9 demonstrated extensive skeletal muscle regeneration in a rat model for volumetric muscle loss (VML) defect.…”

Section: Application Of the Hydrogel-exosome System For Tissue Repair...mentioning

confidence: 99%

Hydrogel-exosome system in tissue engineering: A promising therapeutic strategy

Fan,

Pi,

Zou

et al. 2024

Bioactive Materials

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“…In addition, it is worth noting that the status of donor cells, such as hypoxia culture and inflammatory stimulation, can greatly affect the function of EVs. [10,11] Therefore, it is critical Scheme 1. A schematic illustration of the fabrication and application of the 3D printing Gel/HA/nHAP composite scaffolds.…”

Section: Introductionmentioning

confidence: 99%

“…In addition, it is worth noting that the status of donor cells, such as hypoxia culture and inflammatory stimulation, can greatly affect the function of EVs. [ 10,11 ] Therefore, it is critical to select the appropriate cells according to the therapeutic purpose. In the microenvironment of DM, pathophysiological mechanisms involving cellular dysfunction, inflammation, hypoxia, neuropathy, impaired angiogenesis, and neovascularization will impair the healing process.…”

Section: Introductionmentioning

confidence: 99%

Sustained Release of BMSC‐EVs from 3D Printing Gel/HA/nHAP Scaffolds for Promoting Bone Regeneration in Diabetic Rats

Yang

Dong

Wan

et al. 2023

Adv Healthcare Materials

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Extracellular vesicles (EVs) play an important role in intercellular communication, and the function of EVs mainly depends on the state of source cells. To determine the effect of diabetic microenvironment on EVs secreted by bone marrow mesenchymal stem cells (BMSCs), this work explores the effect of normal glucose (5.5 mm) cultured BMSCs derived EVs (NG-EVs) and high glucose (30 mm) cultured BMSCs derived EVs (HG-EVs) in regulating the migration, proliferation and osteoblastic differentiation of BMSCs in vitro. In order to improve the bioavailability of EVs, this work constructs a sustained release system of polydopamine (PDA) functionalized 3D printing gelatin/hyaluronic acid/nano-hydroxyapatite (Gel/HA/nHAP) scaffolds (S/PDA) and verifies its function in the calvarial defect model of diabetic rats. This work confirms that both NG-EVs and HG-EVs can promote proliferation and migration, inhibit apoptosis and promote osteogenic differentiation, but the function of HG-EVs is weaker than that of NG-EVs. Therefore, EVs secreted by autologous cells of diabetic patients are not suitable for self-repair. This work hopes that the 3D printing scaffold designed for sustained-release EVs will provide a new strategy for acellular tissue engineering bone repair in diabetic patients.

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Exosomes secreted by hypoxia-stimulated bone-marrow mesenchymal stem cells promote grafted tendon-bone tunnel healing in rat anterior cruciate ligament reconstruction model

Cited by 23 publications

References 52 publications

Biomaterial‐Facilitated Local Delivery of Stem Cell‐Derived Small Extracellular Vesicles: Perspectives in Surgical Therapy

Biomaterial‐Facilitated Local Delivery of Stem Cell‐Derived Small Extracellular Vesicles: Perspectives in Surgical Therapy

Hydrogel-exosome system in tissue engineering: A promising therapeutic strategy

Sustained Release of BMSC‐EVs from 3D Printing Gel/HA/nHAP Scaffolds for Promoting Bone Regeneration in Diabetic Rats

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