Expandable progenitors from induced pluripotent stem cells

Masuda, Shigeo; Miyagawa, Shigeru; Fukushima, Satsuki; Nakamura, Toshiharu; Khurram, Maaz Asher; Ishikawa, Tsuyoshi; Saito, Atsuhiro; Sawa, Yoshiki

doi:10.1038/nrcardio.2016.129

Cited by 5 publications

(3 citation statements)

References 10 publications

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“…Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) have the ability to self‐renew and differentiate into almost all types of somatic cells in vitro [1,2]. Therefore, ESCs and iPSCs could be used for a broad range of clinical applications in regenerative medicine [3–6]. It has been reported that genetic and epigenetic networks synergistically maintain the self‐renewal status of human embryonic stem cells (hESCs)/human induced pluripotent stem cells (hiPSCs) [7,8].…”

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confidence: 99%

Iron overload inhibits self‐renewal of human pluripotent stem cells via DNA damage and generation of reactive oxygen species

Han

Chen

et al. 2020

FEBS Open Bio

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Iron overload affects the cell cycle of various cell types, but the effect of iron overload on human pluripotent stem cells has not yet been reported. Here, we show that the proliferation capacities of human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) were significantly inhibited by ferric ammonium citrate (FAC) in a concentration‐dependent manner. In addition, deferoxamine protected hESCs/hiPSCs against FAC‐induced cell‐cycle arrest. However, iron overload did not affect pluripotency in hESCs/hiPSCs. Further, treatment of hiPSCs with FAC resulted in excess reactive oxygen species production and DNA damage. Collectively, our findings provide new insights into the role of iron homeostasis in the maintenance of self‐renewal in human pluripotent stem cells.

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confidence: 99%

Iron overload inhibits self‐renewal of human pluripotent stem cells via DNA damage and generation of reactive oxygen species

Han

Chen

et al. 2020

FEBS Open Bio

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“…3 Also, fibroblast cells are very important in the treatment of various diseases in terms of their ability to transform into other vital cells, such as cardiomyocytes, erythroid progenitors, liver cells, etc. [4][5][6] Thus, the ability of fibroblast cells to differentiate into erythroid progenitors may be used in the ex vivo synthesis of human red blood cells. However, despite the positive potential of these cell lines, red blood cell production on a big scale still has a lot of issues, necessitating the improvement of procedures and research into the impact of numerous (MEMMERT, Italy) with a temperature of 37 °C, 5% CO2, and 95% relative humidity (RH).…”

Section: Introductionmentioning

confidence: 99%

Static electromagnetic field and recombinant human fibroblasts encoding miR-451 and miR-16 increased cell trans-differentiation to CD⁷¹⁺ and CD^235a+ erythroid like progenitor

Karoubi,

Khamisipour,

Babaei

et al. 2023

Bioimpacts

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Introduction: Ex vivo blood production is an urgent need of most countries, and creating production protocols can save the lives of many patients. Despite the recent advances in blood production in ex vivo conditions, its high-scale production is not yet possible, and requires further studies. Therefore, by transfecting fibroblast cells with miR-16, and miR-451 genes, as well as applying low frequency electromagnetic fields (ELF-EMF) treatment, we tried to increase the differentiation of these cells into CD71+ and CD235a+ erythroid like progenitors. Methods: After preparation, and cultivation of human dermal transgenic fibroblast cells, they were transfected by Plenti3-hsa-miR451, Plenti3-hsa-miR16 and Plenti3-backbone inserted into E. coli Stbl4 genome. Then, transgenic fibroblast cells were treated with 10mT ELF-EMF every day for 20 minutes for 7 days. Using a flow cytometer, the expressions of CD71, and CD235a were studied in these cells, and the expressions of genes involved in hematopoiesis were studied using the RT-PCR technique. Results: The results indicated an increase in the differentiation of fibroblast cells treated with 10mT ELF-EMF to erythroid like progenitors. Furthermore, the percentage of CD71+ and CD235a+ cells was the highest in irradiated cells encoding miR-16 and miR-451, which indicates their differentiation into erythroid like progenitors. Also, in the transgenic cells treated with ELF-EMF, an increase in the expressions of α-chain, β-chain, γ-chain and GATA1 genes was observed, which indicates the potential of these cells for hematopoiesis. However, there was no significant difference in the expression of CD34 and CD38 genes in these cell lines. Conclusion: Both ELF-EMF and upregulations of miR-16 and miR-451 lead to improved differentiation of fibroblast cells into erythroid like progenitors.

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“…Human embryonic stem cells (hESCs) have unlimited ability to differentiate into almost all types of somatic cells in vitro ( 1 , 2 ), presenting great potential in a wide range of clinical applications, functional genomics, and developmental biology ( 3 , 4 , 5 ). hESCs are highly prone to cell death upon matrix and cellular detachment through a process referred to as anoikis ( 6 ).…”

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confidence: 99%

Monitoring the induction of ferroptosis following dissociation in human embryonic stem cells

Babaei-Abraki

Karamali

Nasr-Esfahani

2022

Journal of Biological Chemistry

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Expandable progenitors from induced pluripotent stem cells

Cited by 5 publications

References 10 publications

Iron overload inhibits self‐renewal of human pluripotent stem cells via DNA damage and generation of reactive oxygen species

Iron overload inhibits self‐renewal of human pluripotent stem cells via DNA damage and generation of reactive oxygen species

Static electromagnetic field and recombinant human fibroblasts encoding miR-451 and miR-16 increased cell trans-differentiation to CD⁷¹⁺ and CD^235a+ erythroid like progenitor

Monitoring the induction of ferroptosis following dissociation in human embryonic stem cells

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Expandable progenitors from induced pluripotent stem cells

Cited by 5 publications

References 10 publications

Iron overload inhibits self‐renewal of human pluripotent stem cells via DNA damage and generation of reactive oxygen species

Iron overload inhibits self‐renewal of human pluripotent stem cells via DNA damage and generation of reactive oxygen species

Static electromagnetic field and recombinant human fibroblasts encoding miR-451 and miR-16 increased cell trans-differentiation to CD71+ and CD235a+ erythroid like progenitor

Monitoring the induction of ferroptosis following dissociation in human embryonic stem cells

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Static electromagnetic field and recombinant human fibroblasts encoding miR-451 and miR-16 increased cell trans-differentiation to CD⁷¹⁺ and CD^235a+ erythroid like progenitor