Expandase-like activity mediated cell-free conversion of ampicillin to cephalexin by Streptomyces sp. DRS I

Thakur, Debajit; Roy, Monoj K.; Bora, Tarun C.

doi:10.1007/s10529-009-9973-9

Cited by 2 publications

References 16 publications

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Biocatalysis in Organic Media

Illanes

2015

White Biotechnology for Sustainable Chemistry

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Enzyme biocatalysis has evolved from reactions of molecular cleavage in aqueous medium, catalyzed mostly by hydrolases dissolved in the reaction medium, to reactions of molecular synthesis in mostly non-conventional (non-aqueous) media. The latter have a higher potential added value, so most research efforts in recent decades have been devoted to establishing technological platforms for biocatalysis in organic synthesis. Enzymes are evolved catalysts, tailored to perform under physiological conditions with exquisite chemical precision. This attribute has been exploited for making these catalysts apt to perform under the stringent conditions of organic synthesis. Several strategies of enzyme stabilization and activation have been developed and successfully applied to myriad reactions in organic synthesis of potential industrial interest. The conditions for synthesis usually require the reaction to be performed in non-aqueous systems, so enzyme biocatalysis in non-conventional media has been a subject of major attention. Interestingly, robust and readily available hydrolases can catalyze the reverse reactions of synthesis when performed in low water activity environments, opening up the opportunity for these well-known catalysts to be used in high added value processes for the synthesis of fine-chemicals, pharmaceuticals and other high-value bioactive molecules. Advances, potential and the limitations of biocatalysis in non-conventional systems for organic synthesis are reviewed with special emphasis on organic media and their compliance with green chemistry.

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Biocatalysis in Organic Media

Illanes

2015

White Biotechnology for Sustainable Chemistry

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Biosynthesis of active pharmaceuticals: β-lactam biosynthesis in filamentous fungi

Berg

Gidijala²,

Kiel³

et al. 2010

Biotechnology and Genetic Engineering Reviews

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β-lactam antibiotics (e.g. penicillins, cephalosporins) are of major clinical importance and contribute to over 40% of the total antibiotic market. These compounds are produced as secondary metabolites by certain actinomycetes and filamentous fungi (e.g. Penicillium, Aspergillus and Acremonium species). The industrial producer of penicillin is the fungus Penicillium chrysogenum. The enzymes of the penicillin biosynthetic pathway are well characterized and most of them are encoded by genes that are organized in a cluster in the genome. Remarkably, the penicillin biosynthetic pathway is compartmentalized: the initial steps of penicillin biosynthesis are catalyzed by cytosolic enzymes, whereas the two final steps involve peroxisomal enzymes. Here, we describe the biochemical properties of the enzymes of β-lactam biosynthesis in P. chrysogenum and the role of peroxisomes in this process. An overview is given

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Expandase-like activity mediated cell-free conversion of ampicillin to cephalexin by Streptomyces sp. DRS I

Cited by 2 publications

References 16 publications

Biocatalysis in Organic Media

Biocatalysis in Organic Media

Biosynthesis of active pharmaceuticals: β-lactam biosynthesis in filamentous fungi

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