Expanded spectrum of Nocardia species causing clinical nocardiosis detected by molecular methods

Wellinghausen, Nele; Pietzcker, Tim; Kern, Winfried V.; Essig, Andreas; Marre, R.

doi:10.1078/1438-4221-00208

Cited by 32 publications

(28 citation statements)

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“…Moreover, most of these data have not been reported for the previously described N. veterana isolates, precluding comparison between strains. Antimicrobial susceptibility data for N. veterana were reported only once before; these data were consistent with those obtained for strain B430, with the exception of amoxicillin-clavulanate and gentamicin, reported to have intermediate and susceptible MICs, respectively, for the isolate of N. veterana reported by Wellinghausen et al (28).…”

supporting

confidence: 85%

“…Alignment by LALIGN revealed 99.7% homology between the sequences obtained from the Japanese strain and isolate B430. The third isolate of N. veterana was not included in this study, but a 16S rRNA gene identity of 99.1% was observed by the authors with the sequence of the type strain (28). High 16S rDNA sequence similarity values were observed with other species belonging to the genus Nocardia, particularly with Nocardia africana isolates (99.2 and 98.9% for strains with the accession numbers AF277198 and AF302232 and accession numbers AF302230 and AF302231, respectively) and with the Nocardia vaccinii type strain, DSM 43285 T (accession number Z36927; 98%).…”

mentioning

confidence: 97%

“…Although Nocardia asteroides complex and Nocardia brasiliensis are the species most frequently involved in human infections, numerous new species recently characterized within the genus Nocardia have been reported in human clinical samples. Among these, Nocardia veterana was characterized in 2001, and only three clinical isolates have been reported in the literature (11,14,28).…”

mentioning

confidence: 99%

“…Indeed, five new Nocardia species recovered from human samples have been characterized since 2000: N. abscessus (31), N. africana (12), N. cyriacigeorgici (32), N. paucivorans (30), and N. veterana (11). Most of them are implicated in infectious processes: strains belonging to N. africana, N. cyriacigeorgici, and N. paucivorans were isolated from sputa or bronchial secretions of patients with pulmonary infections; N. paucivorans was also reported to have been isolated from the cerebrospinal fluid of a patient with relapse of cerebral nocardiosis (10) and from the central nervous system biopsy specimen of a patient with brain abscesses (28), and N. abscessus was isolated from various abscesses (31) and from a pericardial aspirate (28). Little is known about N. veterana, since only three isolates have been published.…”

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confidence: 99%

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Nocardia veterana Isolated from Ascitic Fluid of a Patient with Human Immunodeficiency Virus Infection

et al. 2003

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Nocardia veterana is a recently characterized species within the genus Nocardia, and only three human clinical isolates have been reported for this species. We describe a case of ascitic fluid infection in an immunocompromised patient due to N. veterana. To our knowledge, this is the first report of a Nocardia sp. strain from ascitic fluid and the fourth report of N. veterana isolated from human samples. Chemotaxonomic methods showed the strain to belong to the genus Nocardia, and identification to the species level was done by 16S ribosomal DNA gene sequencing. The antibiotic susceptibility profile of N. veterana is reported here for the second time. The strain was deposited in the Collection of the Pasteur Institute and in the Culture Collection of the University of Göteborg (CIP 107497 and CCUG 46576). The corresponding 16S ribosomal DNA gene sequence is available from the GenBank database under accession number AY149599. A phylogenetic analysis was conducted and showed that N. veterana was most closely related to the recently characterized species Nocardia africana rather than to Nocardia vaccinii, as previously reported.

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confidence: 97%

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Nocardia veterana Isolated from Ascitic Fluid of a Patient with Human Immunodeficiency Virus Infection

et al. 2003

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“…brasiliensis로 알려져 있다 (Kiska et al, 2002;Roth et al, 2003). 노카르디아증의 진단은 일차적으로 균주의 동정을 통해 이루어지고, 분자 유전학적 기법인 염기서열 분석을 통해 확진될 수 있다 (Wellinghausen et al, 2002;Nolte and Caliendo, 2007). (Fig.…”

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Microbiological Characteristics ofNocardia takedensisIsolated from Skin Lesion, in Korea

Kang

Park

Kim

et al. 2017

BSL

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Nocardia species (spp.) are opportunistic pathogen in immunocompromised hosts. The genus Nocardia contains more than 70 species. Nocardia takedensis has been recently reported as a new species of the genus Nocardia. In this study, we describes the first clinical isolate of N. takedensis from the skin lesion in Busan, Korea. For the identification of clinical isolate to the species level as N. takedensis, classical methods (colony morphology, biochemical characteristics, and antimicrobial susceptibility), molecular method (16S rRNA gene sequencing), and MS (mass spectrometry) analysis were conducted. Clinical isolates grew slowly on the culture media (5% sheep blood agar and chocolate agar) under 5% CO 2 condition. Especially, carotene pigmentation was detected well on the media. Using mass spectrometry, Nocardia isolate was not identified to the species level. However, molecular method based on 16S rRNA sequencing confirmed the isolate as N. takedensis correctly. N. takedensis isolate was partial positive for acid-fast bacilli on the Ziehl-Neelsen method. And it was observed to be resistance to amoxicillin/clavulanic acid and ciprofloxacin. Our results provide useful information to develop optimal identification protocol of N. takedensis in clinical diagnostic laboratories.

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Nocardia, Rhodococcus, Gordonia, Actinomadura, Streptomyces, and Other Aerobic Actinomycetes

Brown‐Elliott,

Zelazny,

Conville

2023

ClinMicroNow

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The anaerobic organisms now in the genus Actinomyces and the aerobic organisms grouped together as the “aerobic actinomycetes” were previously presumed to be related to one another on the basis of shared features of microscopic and colonial morphology. The microscopic and colonial morphologies of organisms in the genus Actinomadura , which contains more than 100 validly named species or subspecies, are very similar to those in the genus Streptomyces . Species in the genus Saccharopolyspora were initially thought to be related to Nocardia and Streptomyces by biochemical characteristics but are phylogenetically distinct from those genera. However, an unknown number of Gordonia infections may be missed, either because the isolate is considered an insignificant coryneform Gram‐positive rod or because the isolate is misidentified by biochemical methods as belonging to another genus, such as Nocardia or Rhodococcus .

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Expanded spectrum of Nocardia species causing clinical nocardiosis detected by molecular methods

Cited by 32 publications

References 12 publications

Nocardia veterana Isolated from Ascitic Fluid of a Patient with Human Immunodeficiency Virus Infection

Nocardia veterana Isolated from Ascitic Fluid of a Patient with Human Immunodeficiency Virus Infection

Microbiological Characteristics ofNocardia takedensisIsolated from Skin Lesion, in Korea

Nocardia, Rhodococcus, Gordonia, Actinomadura, Streptomyces, and Other Aerobic Actinomycetes

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