Expanding the application of anti-CRISPR proteins in plants for tunable genome editing

Liu, Yang; Yuan, Guoliang; Hyden, Brennan; Tuskan, Gerald A.; Abraham, Paul E.; Yang, Xiaohan

doi:10.1093/plphys/kiad076

Cited by 5 publications

(2 citation statements)

References 17 publications

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“…In addition, Liu and their team have shown that transient expression and stable transformation methods can be used to effectively activate AcrIIA4 and AcrIIA5 in herbaceous and woody plant species. The authors used leaf-infiltration and protoplast-based transient expression to investigate the effects of AcrIIA4 and AcrIA5 activities on the SpCas9-based adenine base editor (ABE7) in the herbaceous plants Arabidopsis (Arabidopsis thaliana) and N. benthamiana, as well as the woody plant hybrid poplar "717" (Populus tremula × P. alba hybrid clone INRA 717-1B4), and suggested that both AcrIIA4 and AcrIIA5 are capable of preventing target mutagenesis in the genome of N. benthamiana that is mediated by SpCas9/sgRNA (Liu et al, 2023).…”

Section: Type II Anti-crisprsmentioning

confidence: 99%

“…Acr proteins, in this light, are an underutilized regulatory mechanism in plant biotechnology. In both herbaceous and woody plant species, Acr proteins are effective at inhibiting CRISPR-Cas9based genome-editing tools (Liu et al, 2023). In this review, the numerous types of Acr proteins, their respective mechanisms of action, and the broad range of applications were discussed.…”

Section: Introductionmentioning

confidence: 99%

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A comprehensive appraisal of mechanism of anti-CRISPR proteins: an advanced genome editor to amend the CRISPR gene editing

Choudhary,

Tandi,

Verma

et al. 2023

Front. Plant Sci.

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The development of precise and controlled CRISPR-Cas tools has been made possible by the discovery of protein inhibitors of CRISPR-Cas systems, called anti-CRISPRs (Acrs). The Acr protein has the ability to control off-targeted mutations and impede Cas protein–editing operations. Acr can help with selective breeding, which could help plants and animals improve their valuable features. In this review, the Acr protein–based inhibitory mechanisms that have been adopted by several Acrs, such as (a) the interruption of CRISPR-Cas complex assembly, (b) interference with target DNA binding, (c) blocking of target DNA/RNA cleavage, and (d) enzymatic modification or degradation of signalling molecules, were discussed. In addition, this review emphasizes the applications of Acr proteins in the plant research.

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Section: Type II Anti-crisprsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%