Expanding the chemical diversity of an endophytic fungus<i>Bulgaria inquinans</i>, an ascomycete associated with mistletoe, through an OSMAC approach

Ariantari, Ni Putu; Mándi, Attila; Kurtán, Tibor; Müller, Werner; Lin, Wenhan; Ancheeva, Elena; Proksch, Peter

doi:10.1039/c9ra03678d

Cited by 25 publications

(19 citation statements)

References 67 publications

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“…Alternatively, the identified biosynthetic pathway of the bioactive compounds can be assembled and mimicked in convenient systems, offering an approach to produce target compounds with ease. Second, we need to know more about the roles of host plant–endophyte interactions, requirements of plant niche, and identities of specific signals/elicitors in the synthesis and induction of host-derived natural compounds by endophytes under the OSMAC strategy to overcome the problem of low yield and attenuation, the major challenges for commercial success of this novel approach [ 406 , 408 , 409 , 410 ]. The reasons for the attenuation of products have been attributed to the lack of apparent signals/molecules arising from host–endophyte and/or endophyte–peer endophytes interactions in axenic monocultures, resulting in the switching off of genes [ 86 ].…”

Section: Avenues and Challenges In Application Of Endophyte As Altmentioning

confidence: 99%

Fungal Endophytes as Efficient Sources of Plant-Derived Bioactive Compounds and Their Prospective Applications in Natural Product Drug Discovery: Insights, Avenues, and Challenges

Singh²,

et al. 2021

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Fungal endophytes are well-established sources of biologically active natural compounds with many producing pharmacologically valuable specific plant-derived products. This review details typical plant-derived medicinal compounds of several classes, including alkaloids, coumarins, flavonoids, glycosides, lignans, phenylpropanoids, quinones, saponins, terpenoids, and xanthones that are produced by endophytic fungi. This review covers the studies carried out since the first report of taxol biosynthesis by endophytic Taxomyces andreanae in 1993 up to mid-2020. The article also highlights the prospects of endophyte-dependent biosynthesis of such plant-derived pharmacologically active compounds and the bottlenecks in the commercialization of this novel approach in the area of drug discovery. After recent updates in the field of ‘omics’ and ‘one strain many compounds’ (OSMAC) approach, fungal endophytes have emerged as strong unconventional source of such prized products.

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Section: Avenues and Challenges In Application Of Endophyte As Altmentioning

confidence: 99%

Fungal Endophytes as Efficient Sources of Plant-Derived Bioactive Compounds and Their Prospective Applications in Natural Product Drug Discovery: Insights, Avenues, and Challenges

Singh²,

et al. 2021

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“…The growth of the same strain in solid rice media under static conditions resulted in the production of two new δ-lactone derivatives: photipyrones A and B together with four known analogues of the two compounds that were not produced by the strain grown in SA media [91]. In another study, Bulgaria inquinans, an endophytic fungus isolated from Viscum album, was fermented in solid Czapek medium and later in the same media supplemented with a mixture of MgSO 4 , NaNO 3 and NaCl salts [92]. Numerous compounds were identified from the cultures grown in salt-supplemented Czapek media that were not detectable in cultures grown in normal Czapek media.…”

Section: One-strain Many-compound (Osmac) Approachmentioning

confidence: 99%

Fungal Endophytes: A Promising Frontier for Discovery of Novel Bioactive Compounds

Gakuubi

Madhaiyan

Liang

et al. 2021

JoF

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For years, fungi have served as repositories of bioactive secondary metabolites that form the backbone of many existing drugs. With the global rise in infections associated with antimicrobial resistance, in addition to the growing burden of non-communicable disease, such as cancer, diabetes and cardiovascular ailments, the demand for new drugs that can provide an improved therapeutic outcome has become the utmost priority. The exploration of microbes from understudied and specialized niches is one of the promising ways of discovering promising lead molecules for drug discovery. In recent years, a special class of plant-associated fungi, namely, fungal endophytes, have emerged as an important source of bioactive compounds with unique chemistry and interesting biological activities. The present review focuses on endophytic fungi and their classification, rationale for selection and prioritization of host plants for fungal isolation and examples of strategies that have been adopted to induce the activation of cryptic biosynthetic gene clusters to enhance the biosynthetic potential of fungal endophytes.

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“…using three culture media resulted in six new compounds [ 20 ]. The endophytic fungus Bulgaria inquinans growth in two culture media produced 14 new compounds, including one butyrolactone with high cytotoxic activity [ 21 ]. Although the efficiency of the OSMAC strategy for diversifying the secondary metabolite repertory of one strain is very well documented in literature, this approach is especially interesting for the prospection of compounds of biotechnological interest, such as griseofulvin, an antifungal polyketide produced by several fungi species [ 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

OSMAC Strategy Integrated with Molecular Networking for Accessing Griseofulvin Derivatives from Endophytic Fungi of Moquiniastrum polymorphum (Asteraceae)

et al. 2021

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Three endophytic fungi isolated from Moquiniastrum polymorphum (Less.) G. Sancho (Asteraceae) were cultivated using the one strain many compounds (OSMAC) strategy to evaluate the production of griseofulvin derivatives. Extracts obtained were analyzed by HPLC–MS/MS and the chromatographic and spectrometric data used to elaborate a feature-based molecular network (FBMN) through the GNPS platform. This approach allowed the observation of differences such as medium-specific and strain-specific production of griseofulvin derivatives and variations of cytotoxic activity in most extracts. To evaluate the efficiency of the OSMAC approach allied with FBMN analysis in the prospection of compounds of biotechnological interest, griseofulvin and 7-dechlorogriseofulvin were isolated, and the relative concentrations were estimated in all culture media using HPLC–UV, allowing for the inference of the best strain–medium combinations to maximize its production. Malt extract-peptone broth and Wickerham broth media produced the highest concentrations of both secondary metabolites.

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Expanding the chemical diversity of an endophytic fungusBulgaria inquinans, an ascomycete associated with mistletoe, through an OSMAC approach

Abstract: OSMAC approach on endophytic Bulgaria inquinans by addition of a mixture of salts (MgSO4, NaNO3 and NaCl) to solid Czapek medium induced the accumulation of new secondary metabolites.

Cited by 25 publications

References 67 publications

Fungal Endophytes as Efficient Sources of Plant-Derived Bioactive Compounds and Their Prospective Applications in Natural Product Drug Discovery: Insights, Avenues, and Challenges

Fungal Endophytes as Efficient Sources of Plant-Derived Bioactive Compounds and Their Prospective Applications in Natural Product Drug Discovery: Insights, Avenues, and Challenges

Fungal Endophytes: A Promising Frontier for Discovery of Novel Bioactive Compounds

OSMAC Strategy Integrated with Molecular Networking for Accessing Griseofulvin Derivatives from Endophytic Fungi of Moquiniastrum polymorphum (Asteraceae)

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