Expanding the CRISPR base editing toolbox in Drosophila melanogaster

Clark, Michael; Nguyen, Christina; Nguyen, Hung; Tay, Aidan; Beach, Samuel J.; Maselko, Maciej; López Del Amo, Víctor

doi:10.1038/s42003-024-06848-5

Commun Biol

2024

DOI: 10.1038/s42003-024-06848-5

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Expanding the CRISPR base editing toolbox in Drosophila melanogaster

Michael Clark,

Christina Nguyen,

Hung Nguyen

et al.

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2024

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Cited by 1 publication

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Single-cell transcriptional landscapes ofAedes aegyptimidgut and fat body after a bloodmeal

Vial,

Lopez-Maestre,

Couderc

et al. 2024

Preprint

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Aedes aegypti mosquitoes are vectors for numerous arboviruses that have an increasingly substantial global health burden. Following a bloodmeal, mosquitoes experience significant physiological changes, primarily orchestrated by the midgut and fat body tissues. These changes begin with digestion and culminate in egg production. However, our understanding of those key processes at the cellular and molecular level remains limited. We have created a comprehensive cell atlas of the mosquito midgut and fat body by employing single-cell RNA sequencing and metabolomics techniques. This atlas unveils the dynamic cellular composition and metabolic adaptations that occur following a bloodmeal. Our analyses reveal highly diverse cell populations, specialized in digestion, metabolism, immunity, and reproduction. While the midgut primarily comprises enterocytes, enteroendocrine and intestinal stem cells, the fat body consists not only of trophocytes and oenocytes, but also harbors a substantial hemocyte population and a newly found fat body-yolk cell population. The fat body exhibits a complex cellular and metabolomic profile and exerts a central role in coordinating immune and metabolic processes. Additionally, an insect-specific virus, PCLV (Phasi Charoen-Like Virus) was detected in single cells, mainly in the midgut a week after the bloodmeal. These findings highlight the complexity of the mosquito abdominal tissues, and pave the way towards the development of exquisitely refined vector control strategies consisting of genetically targeting specific cell populations and metabolic pathways necessary for egg development after a bloodmeal.

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Single-cell transcriptional landscapes ofAedes aegyptimidgut and fat body after a bloodmeal

Vial,

Lopez-Maestre,

Couderc

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

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Expanding the CRISPR base editing toolbox in Drosophila melanogaster

Cited by 1 publication

References 62 publications

Single-cell transcriptional landscapes ofAedes aegyptimidgut and fat body after a bloodmeal

Single-cell transcriptional landscapes ofAedes aegyptimidgut and fat body after a bloodmeal

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