Expanding the diversity of oenococcal bacteriophages: Insights into a novel group based on the integrase sequence

Jaomanjaka, Féty; Ballestra, Patricia; Dols‐Lafargue, Marguerite; Marrec, Claire Le

doi:10.1016/j.ijfoodmicro.2013.06.032

Cited by 30 publications

(43 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The complete prophage regions of L . pentosus MP-10 chromosome were identified as Lactobacillus phage Sha1 (region 2; GC content, 40.35%; region length, 39.2 kb) [29] and Oenococcus phage phi 9805 (region 5; GC content, 42.21%; region length, 51.7 kb) [30]. The questionable prophage regions corresponded to Streptococcus pyogenes phage 315.2 (region 1; GC content, 42.18%; region length, 15.4 kb) [29] and Listeria phage B025 (region 4; GC content, 42.96%; region length, 20.9 kb) [31].…”

Section: Resultsmentioning

confidence: 99%

In silico genomic insights into aspects of food safety and defense mechanisms of a potentially probiotic Lactobacillus pentosus MP-10 isolated from brines of naturally fermented Aloreña green table olives

et al. 2017

View full text Add to dashboard Cite

Lactobacillus pentosus MP-10, isolated from brines of naturally fermented Aloreña green table olives, exhibited high probiotic potential. The genome sequence of L. pentosus MP-10 is currently considered the largest genome among lactobacilli, highlighting the microorganism’s ecological flexibility and adaptability. Here, we analyzed the complete genome sequence for the presence of acquired antibiotic resistance and virulence determinants to understand their defense mechanisms and explore its putative safety in food. The annotated genome sequence revealed evidence of diverse mobile genetic elements, such as prophages, transposases and transposons involved in their adaptation to brine-associated niches. In-silico analysis of L. pentosus MP-10 genome sequence identified a CRISPR (clustered regularly interspaced short palindromic repeats)/cas (CRISPR-associated protein genes) as an immune system against foreign genetic elements, which consisted of six arrays (4–12 repeats) and eleven predicted cas genes [CRISPR1 and CRISPR2 consisted of 3 (Type II-C) and 8 (Type I) genes] with high similarity to L. pentosus KCA1. Bioinformatic analyses revealed L. pentosus MP-10 to be absent of acquired antibiotic resistance genes, and most resistance genes were related to efflux mechanisms; no virulence determinants were found in the genome. This suggests that L. pentosus MP-10 could be considered safe and with high-adaptation potential, which could facilitate its application as a starter culture and probiotic in food preparations.

show abstract

Section: Resultsmentioning

confidence: 99%

In silico genomic insights into aspects of food safety and defense mechanisms of a potentially probiotic Lactobacillus pentosus MP-10 isolated from brines of naturally fermented Aloreña green table olives

et al. 2017

View full text Add to dashboard Cite

show abstract

“…As regards the protection against phage attacks, opposite effects have been described: certain EPS are specifically recognized by certain phages and predispose bacteria to the attack by these phages, while others would be a protective barrier [57], [67]. It might be interesting in the future to connect the diversification of eps genes with the high variability in Oenophages recently described [12], [68], [69].…”

Section: Discussionmentioning

confidence: 99%

Exopolysaccharide (EPS) Synthesis by Oenococcus oeni: From Genes to Phenotypes

et al. 2014

Self Cite

View full text Add to dashboard Cite

Oenococcus oeni is the bacterial species which drives malolactic fermentation in wine. The analysis of 50 genomic sequences of O. oeni (14 already available and 36 newly sequenced ones) provided an inventory of the genes potentially involved in exopolysaccharide (EPS) biosynthesis. The loci identified are: two gene clusters named eps1 and eps2, three isolated glycoside-hydrolase genes named dsrO, dsrV and levO, and three isolated glycosyltransferase genes named gtf, it3, it4. The isolated genes were present or absent depending on the strain and the eps gene clusters composition diverged from one strain to another. The soluble and capsular EPS production capacity of several strains was examined after growth in different culture media and the EPS structure was determined. Genotype to phenotype correlations showed that several EPS biosynthetic pathways were active and complementary in O. oeni. Can be distinguished: (i) a Wzy -dependent synthetic pathway, allowing the production of heteropolysaccharides made of glucose, galactose and rhamnose, mainly in a capsular form, (ii) a glucan synthase pathway (Gtf), involved in β-glucan synthesis in a free and a cell-associated form, giving a ropy phenotype to growth media and (iii) homopolysaccharide synthesis from sucrose (α-glucan or β-fructan) by glycoside-hydrolases of the GH70 and GH68 families. The eps gene distribution on the phylogenetic tree was examined. Fifty out of 50 studied genomes possessed several genes dedicated to EPS metabolism. This suggests that these polymers are important for the adaptation of O. oeni to its specific ecological niche, wine and possibly contribute to the technological performance of malolactic starters.

show abstract

“…More recently, two large plasmids, named pOENI-1 (18.3 kb) and pOENI-1v2 (21.9 kb), were described and are presumed to contribute to the fitness of bacteria performing MLF. Finally, the existence of four distinct groups of temperate bacteriophages in O. oeni strains makes prophages a major contributor to the genetic diversity of the O. oeni species [2]. The presence of bacteriophages may play a significant role in shaping microbial communities during the oenological process.…”

Section: Genomementioning

confidence: 99%

Oenococcus oeni: Queen of the cellar, nightmare of geneticists

Grandvalet

2017

Microbiology

View full text Add to dashboard Cite

Graphical abstractThe AbstractOenococcus oeni is a wine-associated lactic acid bacterium (LAB) responsible mostly for wine malolactic fermentation (MLF). This fastidious bacterium (auxotrophic for many amino acids and slow growing) possesses remarkable adaptability to harsh physicochemical conditions and can reprogramme its metabolic pathways to enhance its survival in wine. Thus, O. oeni is an instructive bacterial model for investigating stress response mechanisms in LAB. However, the lack of appropriate techniques to modify the O. oeni genome has hampered molecular studies of this species. The application of recent advances in molecular genetics promises to provide a better understanding of the regulation of stress responses in this species in the future.

show abstract

Expanding the diversity of oenococcal bacteriophages: Insights into a novel group based on the integrase sequence

Cited by 30 publications

References 36 publications

In silico genomic insights into aspects of food safety and defense mechanisms of a potentially probiotic Lactobacillus pentosus MP-10 isolated from brines of naturally fermented Aloreña green table olives

In silico genomic insights into aspects of food safety and defense mechanisms of a potentially probiotic Lactobacillus pentosus MP-10 isolated from brines of naturally fermented Aloreña green table olives

Exopolysaccharide (EPS) Synthesis by Oenococcus oeni: From Genes to Phenotypes

Oenococcus oeni: Queen of the cellar, nightmare of geneticists

Contact Info

Product

Resources

About