Expanding the horizons of genome editing in the fruit fly with Cas12a

Split Proteins and Reassembly Modules for Biological Applications

“…Split inteins, combined with split Cas9, have been employed for translational regulation in the construction of NAND gates, as demonstrated by Kawasaki et al [7b] . In a further demonstration of the versatility of split inteins, precise intersectional labeling was accomplished through the application of a biological AND gate [1b,7a] …”

“…Our focus extends to the latest developments in split protein technology, including Cas proteins [5] and base editors, [6] and explores the realm of conditional splicing systems like split inteins, which have shown significant progress in recent years [1b,7] . By delving into these contemporary advancements, this review bridges the gap between conventional understanding and cutting‐edge applications of split systems in current biological research.…”