Expanding the <i>Trichoderma harzianum</i> species complex: Three new species from Argentine natural and cultivated ecosystems

Barrera, Viviana; Iannone, Leopoldo Javier; Romero, Andrea Irene; Chaverri, Priscila

doi:10.1080/00275514.2021.1947641

Cited by 12 publications

(11 citation statements)

References 42 publications

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“…Trichoderma has been studied for more than 200 years since it was established by Persoon in 1794 [ 12 ], while sharp development occurred in the past few decades, when a large number of taxonomic articles were published [ 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 ]. At present, similar to Fusarium , Aspergillus , or Penicillium , Trichoderma is a species-rich genus [ 15 ] and has been segregated into many groups or clades based on the phylogenetic relationships within the genus [ 27 , 28 , 29 ].…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic Analysis of Trichoderma Species Associated with Green Mold Disease on Mushrooms and Two New Pathogens on Ganoderma sichuanense

Cheng

Gao

et al. 2022

JoF

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Edible and medicinal mushrooms are extensively cultivated and commercially consumed around the world. However, green mold disease (causal agent, Trichoderma spp.) has resulted in severe crop losses on mushroom farms worldwide in recent years and has become an obstacle to the development of the Ganoderma industry in China. In this study, a new species and a new fungal pathogen on Ganoderma sichuanense fruitbodies were identified based on the morphological characteristics and phylogenetic analysis of two genes, the translation elongation factor 1-α (TEF1) and the second-largest subunit of RNA polymerase II (RPB2) genes. The new species, Trichoderma ganodermatigerum sp. nov., belongs to the Harzianum clade, and the new fungal pathogen was identified as Trichoderma koningiopsis. Furthermore, in order to better understand the interaction between Trichoderma and mushrooms, as well as the potential biocontrol value of pathogenic Trichoderma, we summarized the Trichoderma species and their mushroom hosts as best as possible, and the phylogenetic relationships within mushroom pathogenic Trichoderma species were discussed.

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Section: Introductionmentioning

confidence: 99%

Phylogenetic Analysis of Trichoderma Species Associated with Green Mold Disease on Mushrooms and Two New Pathogens on Ganoderma sichuanense

Cheng

Gao

et al. 2022

JoF

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“…After the taxonomic description of Trichoderma endophyticum isolated from Neotropical plants, few other studies have reported this species in other environments [ 50 , 51 , 52 ]. To date, only one study on the application of T. endophyticum is reported in the literature and confirms the status of this species as a growth promoter in Glycine max [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

Characterization of Peptaibols Produced by a Marine Strain of the Fungus Trichoderma endophyticum via Mass Spectrometry, Genome Mining and Phylogeny-Based Prediction

et al. 2023

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Trichoderma is recognized as a prolific producer of nonribosomal peptides (NRPs) known as peptaibols, which have remarkable biological properties, such as antimicrobial and anticancer activities, as well as the ability to promote systemic resistance in plants against pathogens. In this study, the sequencing of 11-, 14- and 15-res peptaibols produced by a marine strain of Trichoderma isolated from the ascidian Botrylloides giganteus was performed via liquid chromatography coupled to high-resolution tandem mass spectrometry (LC-MS/MS). Identification, based on multilocus phylogeny, revealed that our isolate belongs to the species T. endophyticum, which has never been reported in marine environments. Through genome sequencing and genome mining, 53 biosynthetic gene clusters (BGCs) were identified as being related to bioactive natural products, including two NRP-synthetases: one responsible for the biosynthesis of 11- and 14-res peptaibols, and another for the biosynthesis of 15-res. Substrate prediction, based on phylogeny of the adenylation domains in combination with molecular networking, permitted extensive annotation of the mass spectra related to two new series of 15-res peptaibols, which are referred to herein as “endophytins”. The analyses of synteny revealed that the origin of the 15-module peptaibol synthetase is related to 18, 19 and 20-module peptaibol synthetases, and suggests that the loss of modules may be a mechanism used by Trichoderma species for peptaibol diversification. This study demonstrates the importance of combining genome mining techniques, mass spectrometry analysis and molecular networks for the discovery of new natural products.

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“…The genus Trichoderma Pers. (Ascomycota, Sordariomycetes, Hypocreales) is cosmopolitan and of great diversity with over 441 known species until now ( Barrera et al, 2021 ; Zheng et al, 2021 ; Cao et al, 2022 ; Yu et al, 2022 ). Among them, many species, such as T. atroviride , T. harzianum , and T. pleuroticola , are contaminants associated with the production of edible fungi ( Kim et al, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

Diversity of Trichoderma species associated with green mold contaminating substrates of Lentinula edodes and their interaction

Cao,

Zhao,

Liu

et al. 2024

Front. Microbiol.

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IntroductionThe contamination of Trichoderma species causing green mold in substrates poses a significant obstacle to the global production of Lentinula edodes, adversely impacting both yield and quality of fruiting bodies. However, the diversity of Trichoderma species in the contaminated substrates of L. edodes (CSL) in China is not clear. The purpose of this study was to assess the biodiversity of Trichoderma species in CSL, and their interactions with L. edodes.MethodsA comprehensive two-year investigation of the biodiversity of Trichoderma species in CSL was conducted with 150 samples collected from four provinces of China. Trichoderma strains were isolated and identified based on integrated studies of phenotypic and molecular data. Resistance of L. edodes to the dominant Trichoderma species was evaluated in dual culture in vitro.ResultsA total of 90 isolates were obtained and identified as 14 different Trichoderma species, including six new species named as Trichoderma caespitosus, T. macrochlamydospora, T. notatum, T. pingquanense, T. subvermifimicola, and T. tongzhouense, among which, T. atroviride, T. macrochlamydospora and T. subvermifimicola were identified as dominant species in the CSL. Meanwhile, three known species, namely, T. auriculariae, T. paraviridescens and T. subviride were isolated from CSL for the first time in the world, and T. paratroviride was firstly reported to be associated with L. edodes in China. Notebly, the in vitro evaluation of L. edodes resistance to dominant Trichoderma species showed strains of L. edodes generally possess poor resistance to Trichoderma contamination with L. edodes strain SX8 relatively higher resistant.DiscussionThis study systematically investigated the diversity of Trichoderma species in the contaminated substrate of L. edodes, and a total of 31 species so far have been reported, indicating that green mold contaminated substrates of edible fungi were undoubtedly a biodiversity hotspot of Trichoderma species. Results in this study will provide deeper insight into the genus Trichoderma and lay a strong foundation for scientific management of the Trichoderma contamination in L. edodes cultivation.

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Expanding the Trichoderma harzianum species complex: Three new species from Argentine natural and cultivated ecosystems

Cited by 12 publications

References 42 publications

Phylogenetic Analysis of Trichoderma Species Associated with Green Mold Disease on Mushrooms and Two New Pathogens on Ganoderma sichuanense

Phylogenetic Analysis of Trichoderma Species Associated with Green Mold Disease on Mushrooms and Two New Pathogens on Ganoderma sichuanense

Characterization of Peptaibols Produced by a Marine Strain of the Fungus Trichoderma endophyticum via Mass Spectrometry, Genome Mining and Phylogeny-Based Prediction

Diversity of Trichoderma species associated with green mold contaminating substrates of Lentinula edodes and their interaction

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