Expanding the Metabolic Profile of the Fungus Chaetomium sp. through Co‐culture with Autoclaved Pseudomonas aeruginosa

Abstract

The mixed fermentation of the fungal endophyte Chaetomium sp. with an autoclaved culture of the bacterium Pseudomonas aeruginosa on solid rice medium led to an up to 33-fold increase in the accumulation of constitutively present fungal secondary metabolites (3-10), which included four new butenolide derivatives (3-5 and 7). In addition, two further shikimic acid analogues (1 and 2) were obtained from mixed cultures that were neither detected in axenic cultures of the fungus nor of the bacterium. Chaetoisochorismin (1) possesses [a]

Co-culture of the fungusFusarium tricinctumwithStreptomyces lividansinduces production of cryptic naphthoquinone dimers

“…either with B. subtilis or with P. aeruginosa likewise provoked accumulation of different sets of cryptic fungal compounds. 9,10 Future experiments should aim at unravelling the molecular patterns that underlie these specic fungal metabolic responses.…”

“…The aqueous MeOH extract was subjected to a Sephadex LH-20 column, using acetone as mobile phase, to obtain 12 fractions (Fr. [1][2][3][4][5][6][7][8][9][10][11][12][13][14]. The second and third fractions (Fr.2 and 3) were combined due to their similar HPLC proles followed by separation on a silica column.…”

“…For example, treatment of the fungus Chaetomium sp. with autoclaved cultures of Pseudomonas aeruginosa yielded new butenolide derivatives, 9 whereas co-culture of the same fungus with live cultures of B. subtilis yielded new shikimic acid derivatives. 10 Here we have studied the inuence of Streptomyces lividans on accumulation of natural products by F. tricinctum.…”

Lactones from the Sponge-Derived Fungus Talaromyces rugulosus

“…Thus, the planar structure of 1 was elucidated as an unsaturated γ-lactone with propanoic acid n -butyl ester side chain. The absolute configuration of 1 was elucidated by comparing its experimental ECD spectrum with that of WF-3681 methyl ester [ 14 ], which can be considered a close analogue of 1 . Based on the good overall agreement, 1 has ( S ) absolute configuration.…”

“…Thus, compound 2 was identified as 4-methoxylactone acid n -butyl ester. The ECD spectrum of 2 was rather weak and noisy, but similar to that of chaetobutenolide C [ 14 ], which can be used for ECD correlation and indicated a non-racemic mixture of ( R ) and ( S ) isomers with a slight excess of the ( R ) enantiomer.…”