Expanding the role of bitter taste receptor in extra oral tissues: TAS2R38 is expressed in human adipocytes

Cancello, Raffaella; Micheletto, Giancarlo; Meta, Dorela; Lavagno, Rosalia; Bevilacqua, Emanuele; Panizzo, Valerio; Invitti, Cecilia

doi:10.1080/21623945.2019.1709253

Cited by 21 publications

(23 citation statements)

References 32 publications

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“…Alternatively, as human skin is composed of various cell types including melanocytes and adipocytes, both keratinocytes and non‐keratinocyte sources may contribute to the observed levels of TAS2R14 expression. Human subcutaneous adipocytes have been shown to express functional TAS2R38 33 …”

Section: Discussionmentioning

confidence: 99%

Functionally expressed bitter taste receptor TAS2R14 in human epidermal keratinocytes serves as a chemosensory receptor

Bigliardi

Stelmashenko

et al. 2021

Experimental Dermatology

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Traditionally, it is theorized that skin sensation is initiated when cutaneous sensory afferents and Merkel cells receive sensory stimuli, while epidermal keratinocytes were deemed to have no role. However, mounting evidence has shown that keratinocytes can initiate skin sensation by receiving sensory stimuli and transmitting sensory information to sensory afferents. Knowledge regarding the mechanisms by which keratinocytes receive exogenous stimuli is limited, with TRP channels and olfactory receptors having been proposed to serve as receptors for exogenous stimuli in keratinocytes. Recently, expression analyses have demonstrated the expression of multiple TAS2R genes in human skin. TAS2Rs are chemosensory GPCRs employed by taste cells to detect bitter‐tasting substances. However, only subtypes TAS2R1 and TAS2R38 have been characterized in epidermal keratinocytes. We present evidence suggesting that subtype TAS2R14 is functionally expressed in epidermal keratinocytes. TAS2R14 transcripts and protein were detected in primary and N/TERT‐1 keratinocytes. Additionally, keratinocytes responded to α‐thujone, a TAS2R14 ligand, with an increase in intracellular free Ca2+ concentration. The tastant‐evoked Ca2+ signals were found to be mediated by wild‐type TAS2R14 and heterotrimeric G proteins. We conclude that TAS2R14 serves as a chemosensory receptor in epidermal keratinocytes and hypothesize that it enables the cells to recognize potentially harmful chemical substances.

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Section: Discussionmentioning

confidence: 99%

Functionally expressed bitter taste receptor TAS2R14 in human epidermal keratinocytes serves as a chemosensory receptor

Bigliardi

Stelmashenko

et al. 2021

Experimental Dermatology

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“…As their name implies, bitter T2Rs respond to notably bitter compounds and these receptors and their activators have been found throughout the body. Notably, bitter chemicals have been shown to alter food intake via modulation of ghrelin ( Janssen et al, 2011 ), contribute to lipid metabolism ( Cancello et al, 2020 ), and accelerate microorganism clearance in the ciliated airway ( Shah et al, 2009 ). With respect to the liver, bile acids are extremely bitter substances ( Sansome et al, 2020 ), leading to speculation that some of the identified T2Rs respond to these steroid acids that are produced by the liver.…”

Section: Discussionmentioning

confidence: 99%

The Sensing Liver: Localization and Ligands for Hepatic Murine Olfactory and Taste Receptors

et al. 2020

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Sensory receptors, including olfactory receptors (ORs), taste receptors (TRs), and opsins (Opns) have recently been found in a variety of non-sensory tissues where they have distinct physiological functions. As G protein-coupled receptors (GPCRs), these proteins can serve as important chemosensors by sensing and interpreting chemical cues in the environment. We reasoned that the liver, the largest metabolic organ in the body, is primed to take advantage of some of these sensory receptors in order to sense and regulate blood content and metabolism. In this study, we report the expression of novel hepatic sensory receptors – including 7 ORs, 6 bitter TRs, and 1 Opn – identified through a systematic molecular biology screening approach. We further determined that several of these receptors are expressed within hepatocytes, the parenchymal cells of the liver. Finally, we uncovered several agonists of the previously orphaned hepatic ORs. These compounds fall under two classes: methylpyrazines and monoterpenes. In particular, the latter chemicals are plant and fungal-derived compounds with known hepatic protective effects. Collectively, this study sheds light on the chemosensory functions of the liver and unveils potentially important regulators of hepatic homeostasis.

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“…However, as a G protein-coupled receptor, the bitter receptor has seven transmembrane α helixes, which showed difficulty in the isolation and purification of bitter receptors. 52 Thus, the OBPs are excellent alternatives for the construction of bitter biosensors. Besides, the limits of detection using the OBP-based biosensor are even lower than those using bitter taste receptor-based biosensors.…”

Section: Discussionmentioning

confidence: 99%

Detection of Bitter Taste Molecules Based on Odorant-Binding Protein-Modified Screen-Printed Electrodes

et al. 2020

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Bitter taste substances commonly represent a signal of toxicity. Fast and reliable detection of bitter molecules improves the safety of foods and beverages. Here, we report a biosensor using an easily accessible and cost-effective odorant-binding protein (OBP) of Drosophila melanogaster as a biosensitive material for the detection of bitter molecules. Based on the theoretical evaluation of the protein–ligand interaction, binding energies between the OBP and bitter molecules were calculated via molecular docking for the prediction and verification of binding affinities. Through one-step reduction, gold nanoparticles (AuNPs) and reduced graphene oxide (rGO) were deposited on the screen-printed electrodes for improving the electrochemical properties of electrodes. After the electrodes were immobilized with OBPs via layer-by-layer self-assembly, typical bitter molecules, such as denatonium, quinine, and berberine, were investigated through electrochemical impedance spectroscopy. The bitter molecules showed significant binding properties to the OBP with linear response concentrations ranging from 10 –9 to 10 –6 mg/mL. Therefore, the OBP-based biosensor offered powerful analytic techniques for the detection of bitter molecules and showed promising applications in the field of bitter taste evaluation.

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Expanding the role of bitter taste receptor in extra oral tissues: TAS2R38 is expressed in human adipocytes

Cited by 21 publications

References 32 publications

Functionally expressed bitter taste receptor TAS2R14 in human epidermal keratinocytes serves as a chemosensory receptor

Functionally expressed bitter taste receptor TAS2R14 in human epidermal keratinocytes serves as a chemosensory receptor

The Sensing Liver: Localization and Ligands for Hepatic Murine Olfactory and Taste Receptors

Detection of Bitter Taste Molecules Based on Odorant-Binding Protein-Modified Screen-Printed Electrodes

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