Expanding the universe of Piroplasmids: morphological detection and phylogenetic positioning of putative novel piroplasmids in black-eared opossums (Didelphis aurita) from southeastern Brazil, with description of “South American Marsupialia Group” of Piroplasmida

Oliveira, Ágatha Ferreira Xavier de; Calchi, Ana Cláudia; Stocco, Anieli Vidal; Stocco, Naiara Vidal; Costa, Alexandre Carvalho; Mureb, Elisabeth N.; Pires, Jeferson Rocha; Guimarães, Andresa; Raimundo, Juliana Macedo; Balthazar, Daniel de Almeida; Machado, Rosangela Zacarias; André, Marcos Rogério; Baldani, Cristiane Divan

doi:10.1007/s00436-023-07852-0

Cited by 4 publications

(2 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These works demonstrate that the diversity of piroplasmids in wild animals is far from being unraveled. Noteworthily, the present work expands the Piroplasmida phylogenetic clades previously proposed by Jalovecka et al [41], Ikeda et al [11], Mongruel et al [12] and Oliveira et al [13].…”

Section: Discussionsupporting

confidence: 83%

See 1 more Smart Citation

Phylogenetic Inferences Based on Distinct Molecular Markers Confirm a Novel Babesia Species (Babesia goianiaensis nov. sp.) in Capybaras (Hydrochoerus hydrochaeris) and Associated Ticks

et al. 2023

Self Cite

View full text Add to dashboard Cite

Piroplasmids (order Piroplasmida) are a diverse group of tick-borne protozoa that may cause disease in animals and occasionally in humans. Novel Piroplasmida clades and species have been found in wild animals from Brazil based on the phylogenetic assessment of near-complete 18S rRNA, mitochondrial and heat-shock protein genes. For instance, a putative novel Babesia species has been detected in capybaras and Amblyomma ticks in three Brazilian states. The present work aimed to describe, using phylogenetic assessments based on distinct molecular markers, this novel Babesia species in capybaras and associated ticks (Amblyomma sculptum and Amblyomma dubitatum) sampled in Goiânia city, Goiás state, midwestern Brazil. While the phylogenetic analysis based on both near-complete 18S rRNA and hsp-70 genes positioned the sequences obtained from capybara blood samples into a new clade sister to the Babesia sensu stricto clade, the phylogenetic inference based on the COX-3 amino acid positioned the obtained sequences from capybara blood samples and A. sculptum ticks also into a clade sister to the Theileria sensu stricto clade, highlighting the inappropriateness of this marker inferring evolutionary relationships among piroplasmids. Pairwise distance analysis demonstrated that the divergence rates between the 18S rRNA sequences detected in capybaras and other Piroplasmida already described were very high and ranged from 9.4 to 12.9%. Genotype analysis based on the near-full 18S rRNA sequences of the Piroplasmida detected in capybaras and associated ticks demonstrated the occurrence of high genotype diversity at an intra-species level. In conclusion, phylogenetic analyses based on distinct molecular markers supported the description of Babesia goianiaensis nov. sp. in capybaras and associated Amblyomma ticks. Additionally, a novel phylogenetic clade, apart from the previously described ones, was described in the present study and contributed to untangling the complex evolutionary history of the Piroplasmida.

show abstract

Section: Discussionsupporting

confidence: 83%

“…Recently, novel Piroplasmida clades and species have been described in wild animals from Brazil based on the phylogenetic assessment of near-complete 18S rRNA, mitochondrial and heat-shock protein genes [ 11 , 12 , 13 ]. In the same way, a putative novel Babesia sp.…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic Inferences Based on Distinct Molecular Markers Confirm a Novel Babesia Species (Babesia goianiaensis nov. sp.) in Capybaras (Hydrochoerus hydrochaeris) and Associated Ticks

et al. 2023

Self Cite

View full text Add to dashboard Cite

show abstract

Molecular detection of piroplasmids in mammals from the Superorder Xenarthra in Brazil

Calchi,

Yogui,

Alves

et al. 2023

Parasitol Res

View full text Add to dashboard Cite

Tick-borne Apicomplexa in wildlife and ticks of French Guiana

Koual,

de Thoisy,

Baudrimont

et al. 2024

Parasite

View full text Add to dashboard Cite

Tick-borne Apicomplexa encompass a group of parasites responsible for significant medical and veterinary diseases, including babesiosis, theileriosis, and hepatozoonosis. In this study, we investigated the presence and diversity of tick-borne Apicomplexa in wildlife and ticks inhabiting the Amazon rainforests of French Guiana. To this end, we conducted molecular screening and typing using 18S rRNA sequences on a collection of 1161 specimens belonging to 71 species, including 44 species of wild mammals, five species of passerines, and 22 species of ticks. We characterized eight genovariants of Babesia, Theileria, Hemolivia, and Hepatozoon parasites, some matching known species, while others suggested potential novel species. These parasites were detected in wild mammals, including opossums, sloths, armadillos, porcupines, margays, greater grisons, and ticks, but not in passerines. Finally, similarities with surveys conducted in Brazil highlight the specific sylvatic transmission cycles of South American tick-borne Apicomplexa.

show abstract

Expanding the universe of Piroplasmids: morphological detection and phylogenetic positioning of putative novel piroplasmids in black-eared opossums (Didelphis aurita) from southeastern Brazil, with description of “South American Marsupialia Group” of Piroplasmida

Cited by 4 publications

References 58 publications

Phylogenetic Inferences Based on Distinct Molecular Markers Confirm a Novel Babesia Species (Babesia goianiaensis nov. sp.) in Capybaras (Hydrochoerus hydrochaeris) and Associated Ticks

Phylogenetic Inferences Based on Distinct Molecular Markers Confirm a Novel Babesia Species (Babesia goianiaensis nov. sp.) in Capybaras (Hydrochoerus hydrochaeris) and Associated Ticks

Molecular detection of piroplasmids in mammals from the Superorder Xenarthra in Brazil

Tick-borne Apicomplexa in wildlife and ticks of French Guiana

Contact Info

Product

Resources

About