Expansin-mediated developmental and adaptive responses: A matter of cell wall biomechanics?

Samalova, Marketa; Gahurova, Evelina; Hejátko, Jan

doi:10.1017/qpb.2022.6

Cited by 23 publications

(16 citation statements)

References 186 publications

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“…The activity of several CW modifying proteins (CWMPs), such as expansins, is pH-dependent (Cosgrove, 1999 ; Sampedro and Cosgrove, 2005 ). The precise control of H + fluxes and the spatio-temporal specificity of expansins expression and localization are essential in cell elongation (Samalova et al, 2022 ). In fact, salt stress alters the expansins expression pattern in wheat, affecting the cell wall extensibility under different pH apo (Shao et al, 2021 ).…”

Section: The Impact Of Ph Apo On Apoplastic Biologymentioning

confidence: 99%

The root apoplastic pH as an integrator of plant signaling

2022

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Plant nutrition, growth, and response to environmental stresses are pH-dependent processes that are regulated at the apoplastic and subcellular levels. The root apoplastic pH is especially sensitive to external cues and can also be modified by intracellular inputs, such as hormonal signaling. Optimal crosstalk of the mechanisms involved in the extent and span of the apoplast pH fluctuations promotes plant resilience to detrimental biotic and abiotic factors. The fact that variations in local pHs are a standard mechanism in different signaling pathways indicates that the pH itself can be the pivotal element to provide a physiological context to plant cell regions, allowing a proportional reaction to different situations. This review brings a collective vision of the causes that initiate root apoplastic pHs variations, their interaction, and how they influence root response outcomes.

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Section: The Impact Of Ph Apo On Apoplastic Biologymentioning

confidence: 99%

The root apoplastic pH as an integrator of plant signaling

2022

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“…This supports the contention that cell wall loosening and remodeling, apart from its role in normal growth, is also crucial for the adaptation to environmental stresses, especially in roots. Typically, stresses that lead to ROS production and loss of water alter the expression of EXPs (41). However, the precise mechanism of action of EXPs in stress mitigation remains unclear.…”

Section: Discussionmentioning

confidence: 99%

“…S4). They enable cell expansion and increase cell wall flexibility (40,41). The EXP superfamily is divided into four groups: EXPA, EXPB, expansins-like A (EXPLA) and EXPLB.…”

Section: Expansin (Exp) Superfamilymentioning

confidence: 99%

The molecular core of transcriptome responses to abiotic stress in plants: a machine learning-driven meta-analysis

Sanchez-Munoz,

Depaepe,

Samalova

et al. 2024

Preprint

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Understanding how plants adapt their physiology to overcome severe stress conditions is vital in light of the current climate crisis. This remains a challenge given the complex nature of the underlying molecular mechanisms. To provide a full picture of stress mitigation mechanisms, an exhaustive analysis of publicly available stress-related transcriptomic data was conducted. We combined a meta-analysis with an unsupervised machine learning algorithm to identify a core of stress-related genes. To ensure robustness and biological significance of the output, often lacking in meta-analyses, a three-layered biovalidation was incorporated. Our results present a stress gene core, a set of key genes involved in plant tolerance to a multitude of adverse environmental conditions rather than specific ones. In addition, we provide a biologically validated database to assist in design of multi-stress resilience. Taken together, our results pave the way towards future-proof sustainable agriculture.

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“…EXPA1 (At1g69530) has been known for decades from experiments with beads loaded with purified expansin that induced leaf primordia formation on the tomato shoot apical meristem ( Fleming et al 1997 ). Apart from leaf organogenesis ( Reinhardt et al 1998 ) and vascular tissue differentiation ( Cho and Kende 1998 ), expansins are involved in root development and growth ( Lee and Kim 2013 ; Pacifici et al 2018 ; Ramakrishna et al 2019 ), root hair initiation ( Cho and Cosgrove 2002 ), and seed germination ( Ribas et al 2019 ; Sanchez-Montesino et al 2019 ); for more examples, see the recent review by Samalova et al (2022) . Interestingly, NbEXPA1 was shown to be plasmodesmata-specific and functions as a host factor for potyviral infection ( Park et al 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Hormone-regulated expansins: Expression, localization, and cell wall biomechanics in Arabidopsis root growth

et al. 2023

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Expansins facilitate cell expansion by mediating pH-dependent cell wall (CW) loosening. However, the role of expansins in controlling CW biomechanical properties in specific tissues and organs remains elusive. We monitored hormonal responsiveness and spatial specificity of expression and localization of expansins predicted to be the direct targets of cytokinin signaling in Arabidopsis (Arabidopsis thaliana). We found EXPANSIN1 (EXPA1) homogenously distributed throughout the CW of columella/lateral root cap, while EXPA10 and EXPA14 localized predominantly at three-cell boundaries in the epidermis/cortex in various root zones. EXPA15 revealed cell type-specific combination of homogenous vs. three-cell boundaries localization. By comparing Brillouin frequency shift and AFM-measured Young’s modulus, we demonstrated Brillouin light scattering (BLS) as a tool suitable for non-invasive in vivo quantitative assessment of CW viscoelasticity. Using both BLS and AFM, we showed that EXPA1 overexpression upregulated CW stiffness in the root transition zone. The dexamethasone-controlled EXPA1 overexpression induced fast changes in the transcription of numerous CW-associated genes, including several EXPAs and XYLOGLUCAN:XYLOGLUCOSYL TRANSFERASEs (XTHs), and associated with rapid pectin methylesterification determined by in situ Fourier transform infrared spectroscopy in the root transition zone. The EXPA1-induced CW remodeling associated with shortening of the root apical meristem, leading to root growth arrest. Based on our results, we propose that expansins control root growth by a delicate orchestration of CW biomechanical properties, possibly regulating both CW loosening and CW remodeling.

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Expansin-mediated developmental and adaptive responses: A matter of cell wall biomechanics?

Cited by 23 publications

References 186 publications

The root apoplastic pH as an integrator of plant signaling

The root apoplastic pH as an integrator of plant signaling

The molecular core of transcriptome responses to abiotic stress in plants: a machine learning-driven meta-analysis

Hormone-regulated expansins: Expression, localization, and cell wall biomechanics in Arabidopsis root growth

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