Expansion and countrywide dissemination of ST11, ST15 and ST147 ciprofloxacin-resistant CTX-M-15-type  -lactamase-producing Klebsiella pneumoniae epidemic clones in Hungary in 2005--the new 'MRSAs'?

Damjanova, Ivelina; Tóth, Ákos; Pászti, Judit; Hajbel-Vékony, Gabriella; Jakab, Melinda; Berta, Judit; Milch, H; Füzi, M

doi:10.1093/jac/dkn287

Cited by 194 publications

(170 citation statements)

References 24 publications

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“…Two of the epidemic clones described (ST11 and ST274) were not observed during our study period, and despite widespread dissemination of ST147 across the country in 2005, it was only detected fi rst in 2007 in our institution possibly due to later importation or low incidence rates [6,7]. According to our investigations ST15 and CTX-M-15 β-lactamases were the dominant clone and ESBL type in our institution, which matches with national data [6].…”

Section: Acta Microbiologica Et Immunologica Hungarica 62 2015supporting

confidence: 87%

“…Beside several minor clones, three internationally disseminated CTX-M-15 producing K. pneumoniae clones, namely ST15, ST101 and ST147 were present in the Clinical Centre University of Pécs, of which ST15 and ST147 are considered to be epidemic clones in Hungary [6,[32][33][34][35][36].…”

Section: Discussionmentioning

confidence: 99%

“…Clonal relationships were assessed with pulsed-fi eld gel electrophoresis (PFGE) as described by others [6]. PFGE was evaluated with unweighted pair group method with arithmetic means (UPGMA) using Dice-coeffi cient.…”

Section: Molecular Typingmentioning

confidence: 99%

“…In 2005 the spread of three CTX-M-15 producing epidemic clones (ST11, ST15 and ST147) were detected across the country [6]. One year later the emergence of a fourth epidemic clone (ST274) was observed in various regions of the country [7].…”

Section: Introductionmentioning

confidence: 99%

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Identification and characterization of CTX-M-15 producing Klebsiella pneumoniae clone ST101 in a Hungarian university teaching hospital

Melegh

Schneider

Horváth

et al. 2015

Acta Microbiologica et Immunologica Hungarica

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We investigated the molecular epidemiology of extended spectrum β-lactamase (ESBL) producing Klebsiella pneumoniae isolates derived from the teaching hospitals of University of Pécs, Pécs, Hungary in the time period [2004][2005][2006][2007][2008]. Molecular typing, antimicrobial susceptibility testing, detection of common β-lactamase genes (bla CTX-M , bla TEM and bla SHV ) and virulence associated traits (hypermucoviscosity, magA, k2a, rmpA, siderophores, type 1 and 3 fi mbria, biofi lm formation, serum resistance) were performed for 102 isolates. The results showed the presence of three major ciprofl oxacin resistant CTX-M-15 producing clones (ST15 n = 69, ST101 n = 10, and ST147 n = 9), of which ST15 was predominant and universally widespread. Considering distribution in time and place, ST101 and ST147 were detected at fewer inpatient units and within a narrower time frame, as compared to ST15. Beside major clones, eleven minor clones were identifi ed, and were shown to harbour the following β-lactamase genes: six clones carried bla CTX-M , four clones harboured bla SHV-5 and one clone possessed both bla CTX-M and ESBL type bla SHV . Among the SHV-5 producing K. pneumoniae clones a novel sequence type was found, namely ST1193, which harboured a unique infB allele. Different virulence factor content and peculiar antimicrobial susceptibility profi le were characteristic for each clone. In contrast to major clone isolates, which showed high level resistance to ciprofl oxacin, minor clone *Corresponding author; E-mail: melegh.szilvia@pte.hu 234 MELEGH et al.Acta Microbiologica et Immunologica Hungarica 62, 2015 isolates displayed signifi cantly lower MIC values for ciprofl oxacin suggesting a role for fl uoroquinolones in the dissemination of the major K. pneumoniae clones. This is the fi rst description of the CTX-M-15 producing K. pneumoniae clone ST101 in Hungary.

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Section: Acta Microbiologica Et Immunologica Hungarica 62 2015supporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Section: Molecular Typingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Identification and characterization of CTX-M-15 producing Klebsiella pneumoniae clone ST101 in a Hungarian university teaching hospital

Melegh

Schneider

Horváth

et al. 2015

Acta Microbiologica et Immunologica Hungarica

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“…A bla VIM -specific probe hybridised strongly with a band corresponding to the chromosomal material, which confirmed the chromosomal location of the bla VIM-1 -containing integron. All isolates belonged to ST11, which is a common clone of K. pneumoniae that possesses extended spectrum (ESBL)-and AmpC-beta-lactamases [25,26]. The sixth VIM-4-producing strain was detected in October 2011 in a patient admitted to the hospital in the Czech Republic after the medically assisted repatriation from a hospital in Northern Greece.…”

Section: Vim-producing Isolatesmentioning

confidence: 99%

Carbapenemase-producing Klebsiella pneumoniae in the Czech Republic in 2011

et al. 2013

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Carbapenemase-producing Enterobacteriaceae and Pseudomonas spp. are increasingly reported in many countries all over the world. Due to the resistance of those bacteria to almost all antibiotics (e.g. beta-lactams, aminoglycosides, fluoroquinolones), treatment options are seriously limited. In the Czech Republic, the incidence of carbapenemase-producing Enterobacteriaceae seems to be low, restricted to only three cases detected between 2009 and 2010. Here, we describe molecular typing of 15 carbapenemase-producing Klebsiella pneumoniae isolates identified in the Czech Republic during 2011. Five VIM-1-producing isolates belonging to sequence type (ST) 11 and one VIM-4-producing isolate of ST1029 have been detected. blaVIM-1 and blaVIM-4 as a part of class 1 integrons were chromosomally located or carried by a plasmid belonging to A/C replicon type (blaVIM-4). KPC-3-producing isolates of ST512, recovered from six patients, caused an outbreak. Three more isolates producing KPC-2 enzyme belonged to ST258. Both blaKPC genes were part of the Tn4401a transposon carried on plasmids of the pKpQIL type. The isolates were resistant to all antibiotics tested except colistin and/or gentamicin. Four of these 15 strains were recovered from patients repatriated to the Czech Republic from Greece and Italy. This is the first report of outbreaks caused by carbapenemase-producing Enterobacteriaceae in the Czech Republic.

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Novel arrangement of thebla_CTX-M-55gene in anEscherichia coliisolate coproducing 16S rRNA methylase

Pan

Liu

et al. 2013

J. Basic Microbiol.

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A multi-drug resistant Escherichia coli C21 was isolated from a chicken in China. It was shown to be positive for the presence of the blaTEM-1, blaCTX-M-55 and rmtB genes by PCR. This strain was examined by phylogenetic grouping, conjugation experiments, plasmid analysis, PCR-based replicon typing and multi-locus sequence typed (MLST). The genetic environment of blaCTX-M-55 was investigated by PCR mapping. The strain belonged to phylogroup A, ST156. The blaCTX-M-55 and rmtB genes were found to be present in separate plasmids that belonged to the IncI1 and IncN families, respectively. These antibiotic-resistant plasmids could be transferred to the recipient strain alone or together. A new arrangement of ISEcp1Δ-IS1294-ΔISEcp1-blaCTX-M-55 -ORF477, in which the ISEcp1 element was disrupted by another IS1294 element, was identified initially. Conjugative transfer and IS elements found in this study could lead to the rapid dissemination of blaCTX-M-55 and rmtB among strains of Enterobacteriaceae, which could pose a threat to animal husbandry and public health.

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Expansion and countrywide dissemination of ST11, ST15 and ST147 ciprofloxacin-resistant CTX-M-15-type -lactamase-producing Klebsiella pneumoniae epidemic clones in Hungary in 2005--the new 'MRSAs'?

Abstract: In 2005, 97% of all CTX-M-producing K. pneumoniae isolates detected across Hungary were highly ciprofloxacin-resistant CTX-M-15 producers and represented just three stable genetic clones.

Cited by 194 publications

References 24 publications

Identification and characterization of CTX-M-15 producing Klebsiella pneumoniae clone ST101 in a Hungarian university teaching hospital

Identification and characterization of CTX-M-15 producing Klebsiella pneumoniae clone ST101 in a Hungarian university teaching hospital

Carbapenemase-producing Klebsiella pneumoniae in the Czech Republic in 2011

Novel arrangement of thebla_CTX-M-55gene in anEscherichia coliisolate coproducing 16S rRNA methylase

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Expansion and countrywide dissemination of ST11, ST15 and ST147 ciprofloxacin-resistant CTX-M-15-type -lactamase-producing Klebsiella pneumoniae epidemic clones in Hungary in 2005--the new 'MRSAs'?

Abstract: In 2005, 97% of all CTX-M-producing K. pneumoniae isolates detected across Hungary were highly ciprofloxacin-resistant CTX-M-15 producers and represented just three stable genetic clones.

Cited by 194 publications

References 24 publications

Identification and characterization of CTX-M-15 producing Klebsiella pneumoniae clone ST101 in a Hungarian university teaching hospital

Identification and characterization of CTX-M-15 producing Klebsiella pneumoniae clone ST101 in a Hungarian university teaching hospital

Carbapenemase-producing Klebsiella pneumoniae in the Czech Republic in 2011

Novel arrangement of theblaCTX-M-55gene in anEscherichia coliisolate coproducing 16S rRNA methylase

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Novel arrangement of thebla_CTX-M-55gene in anEscherichia coliisolate coproducing 16S rRNA methylase