Expansion and osteogenic differentiation of bone marrow-derived mesenchymal stem cells on a vitamin C functionalized polymer

Wang, Yongzhong; Singh, Àmarjit; Xu, Peng; Pindrus, Mariya A.; Blasioli, Dominick J.; Kaplan, David L.

doi:10.1016/j.biomaterials.2006.01.036

Cited by 47 publications

(35 citation statements)

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“…An aliquot of RNA (500 ng) was reverse-transcribed into cDNA by oligo(dT) [12][13][14][15][16][17][18] primer. Real time PCR contained 100 ng of template cDNA, 1ϫ SYBR GREEN master mix (Qiagen), and 100 nM specific forward and reverse primers in a 25-l volume of reaction.…”

Section: Methodsmentioning

confidence: 99%

“…Deficiency of AA is responsible for scurvy syndrome, which has characteristics of the defective synthesis of collagen (10). Moreover, it has been shown that AA induces embryonic and mesenchymal stem cells to differentiate into osteoblasts (11,12). The potential mechanisms by which AA directs the multipotent progenitor cell commitments are believed to be mediated through collagen matrix synthesis, cell-matrix interaction, and activation of integrin signaling (11,13).…”

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confidence: 99%

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Nuclear Factor-E2-related Factor-1 Mediates Ascorbic Acid Induction of Osterix Expression via Interaction with Antioxidant-Responsive Element in Bone Cells

Xing

Singgih

Kapoor

et al. 2007

Journal of Biological Chemistry

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We recently found that deletion of the gulonolactone oxidase gene, which is involved in the synthesis of ascorbic acid (AA), was responsible for the fracture phenotype in spontaneous fracture mice. To explore the molecular mechanisms by which AA regulates osteoblast differentiation, we examined the effect of AA on osterix expression via Nrf1 (NF-E2-related factor-1) binding to antioxidant-responsive element (ARE) in bone marrow stromal (BMS) cells. AA treatment caused a 6-fold increase in osterix expression in mutant BMS cells at 24 h, which was unaffected by pretreatment with protein synthesis inhibitor. Sequence analyses of mouse osterix promoter revealed a putative ARE located at ؊1762 to ؊1733 upstream of the transcription start site to which Nrf potentially binds. A gel mobility shift assay revealed that nuclear proteins from AA-treated BMS cells bound to radiolabeled ARE much more strongly than nuclear extracts from AA-untreated cells. A chromatin immunoprecipitation assay with Nrf1 antibody confirmed the interaction of Nrf1 with the mouse osterix promoter.

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Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

Nuclear Factor-E2-related Factor-1 Mediates Ascorbic Acid Induction of Osterix Expression via Interaction with Antioxidant-Responsive Element in Bone Cells

Xing

Singgih

Kapoor

et al. 2007

Journal of Biological Chemistry

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“…It is an indispensable supplement for promoting the proliferation and differentiation of a variety of mesenchymal stem cells by enhancing collagen biosynthesis, ALP activity, the expression of BSP and other extracellular matrix production (Franceschi et al, 1994;Shiga et al, 2003;Wang et al, 2006;Cao et al, 2012;Mekala et al, 2013). Evidences also indicate that VC can induce iPSCs (induced pluripotent stem cells) differentia-& tion and malignant cells apoptosis via ERK/MAPK pathway (Park et al, 2005;Cao et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Vitamin C induces periodontal ligament progenitor cell differentiation via activation of ERK pathway mediated by PELP1

et al. 2013

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The differentiation of periodontal ligament (PDL) progenitor cells is important for maintaining the homeostasis of PDL tissue and alveolar bone. Vitamin C (VC), a water-soluble nutrient that cannot be biosynthesized by humans, is vital for mesenchymal stem cells differentiation and plays an important role in bone remodeling. Therefore, the objective of this study was to determine the function and mechanism of VC in PDL progenitor cells osteogenic differentiation at the molecular level. We demonstrated that VC could induce the osteogenic differentiation and maturation of PDL progenitor cell without other osteogenic agents. During the process, VC preferentially activated ERK1/2 but did not affect JNK or p38. Co-treatment with ERK inhibitor effectively decreased the Vitamin C-induced expression of Runx2. ERK inhibitor also abrogated Vitamin C-induced the minimized nodules formation. PELP1, a nuclear receptor co-regulator, was up-regulated under VC treatment. PELP1 knockdown inhibited ERK phosphorylation. The overexpression of PELP1 had a positive relationship with Runx2 expression. Taken together, we could make a conclude that VC induces the osteogenic differentiation of PDL progenitor cells via PELP1-ERK axis. Our fi nding implies that VC may have a potential in the regeneration medicine and application to periodontitis treatment.

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“…It has been reported that chondrocytes increased in number by 100-fold within 17 days when a special growth factor cocktail was used in the culture medium ). Also, ultra-nanocrystalline diamond films have been shown to enhance neural stem cell expansion for tissue engineering (Wang et al, 2006). Moreover other growth factors that have been used to enhance stem cell expansion include ascorbic acid-functionalized poly (methyl methacrylate) (AA-PMMA) (Tamama et al, 2005); epidermal growth factor receptor (EGFR) (Hsieh-Bonassera et al, 2009) ;and Flt3 ligand (FL) was used in addition to IL-11 or G-SCF (Granulocyte-Colony Stimulating factor) to stimulate the growth of rat MSCs (Jacobsen et al, 1995).…”

Section: Lipus Utilization In Cell Expansionmentioning

confidence: 99%

Low Intensity Pulsed Ultrasound: A Laboratory and Clinical Promoter in Tissue Engineering Abstract

El‐Bialy¹

2010

Tissue Engineering

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This chapter will focus on the current and future applications of low intensity pulsed ultrasound (LIPUS) in tissue engineering at laboratory and clinical levels. Also, this chapter will explain the molecular basis of LIPUS stimulatory effects on cell expansion, differentiation and matrix production of different tissues. In addition, this chapter will provide information about the differences between LIPUS application in the laboratory settings and in the clinical applications with emphasis on tissue engineering applications. Moreover, this chapter will discuss why variability in outcome might occur when applying LIPUS in laboratory or clinical settings. Also, we will provide some tips on how to prevent potential inconsistency in treatment results with emphasis on the technique sensitivity and how a scientist or a clinician should be aware of this sensitivity of the application technique in order to optimize the utilization of LIPUS in different settings. This chapter will set a few hypotheses and potential new applications of utilizing LIPUS in other tissue engineering applications. The outcome of this chapter will be an understanding of the advantages and limitations of using LIPUS in tissue engineering applications either at the laboratory or in clinical settings.

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Expansion and osteogenic differentiation of bone marrow-derived mesenchymal stem cells on a vitamin C functionalized polymer

Cited by 47 publications

References 36 publications

Nuclear Factor-E2-related Factor-1 Mediates Ascorbic Acid Induction of Osterix Expression via Interaction with Antioxidant-Responsive Element in Bone Cells

Nuclear Factor-E2-related Factor-1 Mediates Ascorbic Acid Induction of Osterix Expression via Interaction with Antioxidant-Responsive Element in Bone Cells

Vitamin C induces periodontal ligament progenitor cell differentiation via activation of ERK pathway mediated by PELP1

Low Intensity Pulsed Ultrasound: A Laboratory and Clinical Promoter in Tissue Engineering Abstract

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