Expansion microscopy of neutrophil nuclear structure and extracellular traps

Holsapple, Jason S.; Schnitzler, Lena; Rusch, Louisa; Baldeweg, Tobias Horst; Neubert, Elsa; Kruss, Sebastian; Erpenbeck, Luise

doi:10.1016/j.bpr.2022.100091

Cited by 5 publications

(1 citation statement)

References 49 publications

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“…How nuclear deformation impacts cell functions is an intricate question because it appears in both healthy and diseased contexts. On the one hand, nuclear deformation is a benign morphological characteristic commonly observed in various cell types, such as stem cells and neutrophils 2–4 . On the other hand, severe nuclear deformations, such as deep nuclear invaginations, are pathological hallmarks associated with cancers 5 , neurodegeneration 6 , progeria 7 , normal aging 8 , and viral infections 9 .…”

Section: Introductionmentioning

confidence: 99%

Coaching ribosome biogenesis from the nuclear periphery

Zhuang,

Guo,

Razorenova

et al. 2024

Preprint

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SummarySevere invagination of the nuclear envelope is a hallmark of cancers, aging, neurodegeneration, and infections. However, the outcomes of nuclear invagination remain unclear. This work identified a new function of nuclear invagination: regulating ribosome biogenesis. With expansion microscopy, we observed frequent physical contact between nuclear invaginations and nucleoli. Surprisingly, the higher the invagination curvature, the more ribosomal RNA and pre-ribosomes are made in the contacted nucleolus. By growing cells on nanopillars that generate nuclear invaginations with desired curvatures, we can increase and decrease ribosome biogenesis. Based on this causation, we repressed the ribosome levels in breast cancer and progeria cells by growing cells on low-curvature nanopillars, indicating that overactivated ribosome biogenesis can be rescued by reshaping nuclei. Mechanistically, high-curvature nuclear invaginations reduce heterochromatin and enrich nuclear pore complexes, which promote ribosome biogenesis. We anticipate that our findings will serve as a foundation for further studies on nuclear deformation.HighlightsNuclear invaginations regulate ribosome biogenesis by physically contacting nucleoli.High-curvature nuclear tunnels increase ribosome biogenesis.Nanopillars reduce ribosome biogenesis by transforming high-curvature nuclear invaginations to low-curvature ones.

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Section: Introductionmentioning

confidence: 99%

Coaching ribosome biogenesis from the nuclear periphery

Zhuang,

Guo,

Razorenova

et al. 2024

Preprint

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Imaging and Therapy of Tumors Based on Neutrophil Extracellular Traps

Hao,

Liu,

Wang

et al. 2024

Small Science

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Neutrophil extracellular traps (NETs) formed by neutrophils are netlike scaffolds that mainly contain DNA and a variety of granule proteins. Many stimuli can lead to the NET formation through independent molecular pathways. Clinically, the abundance of NETs is correlated with poor tumor prognosis. The biological actions of NETs are complex and diverse, including promoting tumor progression, awakening the dormant cancer cells, and resulting in immunosuppression in support of tumor growth and metastasis. Therefore, NET‐associated pathological processes provide an important clue for both diagnostic imaging and alternative therapies for many kinds of cancers. In recent years, scientists’ efforts have focused on developing novel imaging probes to visualize NETs and therapeutic strategies by degrading NETs or inhibiting its formation to block their pro‐tumoral functions. In this review, the development and evaluation of NETs‐targeted imaging and intervention progress for tumor therapy are focused on.

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Microscopy methods to visualize nuclear organization in biomechanical studies

Kim,

Lakadamyali

2024

Current Opinion in Biomedical Engineering

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Expansion microscopy of neutrophil nuclear structure and extracellular traps

Cited by 5 publications

References 49 publications

Coaching ribosome biogenesis from the nuclear periphery

Coaching ribosome biogenesis from the nuclear periphery

Imaging and Therapy of Tumors Based on Neutrophil Extracellular Traps

Microscopy methods to visualize nuclear organization in biomechanical studies

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