Expansion of an Unusual Population of Gr-1+CD3intCells in the Lymph Nodes and Other Peripheral Organs of Mice Carrying thelprGene

Honda, Shigeru; Takeda, Kazuyoshi; Narita, Junichi; Koya, Toshiyuki; Kawamura, Toshihiko; Kuwano, Yuh; Watanabe, Hirotaka; Arakawa, Masaaki; Abo, Toru

doi:10.1006/cimm.1997.1104

Cited by 8 publications

(7 citation statements)

References 22 publications

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“…Further experiments showed that the GR-1 lo (or Ly-6C lo ) PEC in B6/gld and B6/lpr mice coexpressed B220 (CD45R) and Thy-1 (CD90), which suggests that these GR-1 lo PEC represent the abnormal DN T cells that accumulate in the spleen and lymph nodes of B6/gld and B6/lpr mice as their autoimmune disease progresses. Thus, as shown previously by other laboratories [47][48][49][50], and confirmed by studies from our laboratory (data not shown), the Ly-6C molecule can be added to the list of cell surface markers that are expressed inappropriately on DN T cells from B6/gld and B6/lpr mice.…”

Section: Fas Expression On Gr-1 Hi Inflammatory Neutrophilssupporting

confidence: 85%

Fas ligand (gld)- and Fas (lpr)-deficient mice do not show alterations in the extravasation or apoptosis of inflammatory neutrophils

Fecho

Cohen

1998

Journal of Leukocyte Biology

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Apoptosis of neutrophils plays a critical role in the resolution of acute inflammation. Neutrophils from human peripheral blood express Fas (CD95) and are sensitive to Fas ligand (FasL)/Fasmediated apoptosis. Mice carrying spontaneous mutations in the genes for fas ligand (B6/gld) or fas (B6/lpr) were used to assess the role of FasL/Fas in the kinetics and magnitude of neutrophil extravasation to the thioglycolate (TG)-inflamed peritoneum and in the spontaneous apoptosis of TGelicited neutrophils. The results showed that TG-elicited neutrophils (defined by flow cytometry as GR-1/Ly-6G hi cells) from normal (B6) and B6/ gld mice, but not from the Fas-deficient B6/lpr mice, express high levels of Fas. The TG-elicited neutrophil response began at 2 h, peaked at 4 h, and subsided by 24-48 h after TG administration in all three strains. However, the response was more prolonged in B6 mice, such that B6/gld and B6/lpr mice had fewer neutrophils at 6 h after TG administration than did B6 mice. Further studies showed that 4 h TG-elicited neutrophils from B6, B6/gld and B6/lpr mice undergo apoptosis in vitro at similar rates (as assessed through flow cytometry by the decrease in forward angle light-scatter and externalization of phosphatidylserine (PS; as detected by Annexin V-FITC) that occur as neutrophils undergo apoptosis). Fas expression was downregulated on apoptotic neutrophils in conjunction with maximal PS externalization and decreased forward angle light-scatter. Collectively, these findings suggest that FasL/Fas-mediated apoptosis is not essential in regulating the lifespan of neutrophils during an acute inflammatory response. The abbreviated inflammatory response observed in FasL/Fasdeficient mice is likely to be a secondary effect of the gld/lpr autoimmune/lymphoproliferative syndrome, and not a direct effect of FasL/Fas on the ability of inflammatory neutrophils to undergo apoptosis.

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Section: Fas Expression On Gr-1 Hi Inflammatory Neutrophilssupporting

confidence: 85%

Fas ligand (gld)- and Fas (lpr)-deficient mice do not show alterations in the extravasation or apoptosis of inflammatory neutrophils

Fecho

Cohen

1998

Journal of Leukocyte Biology

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“…At this time, the number of lymphocytes was not correlated with others. However, we did not observe the induction of erythrocyte differentiation when G-CSF was administered into mice in the previous study (8).…”

Section: Phocytes (Ie Nk Cells and Extrathymic T Cells)contrasting

confidence: 58%

“…(manuscript in preparation). This is due to the expression of G-CSF receptors on NK cells and extrathymic T cells (8). Similarly, we encountered the fact that the number of granulocytes (mainly neutrophils) and that of erythrocytes were intimately correlated with each other in healthy individuals.…”

Section: Phocytes (Ie Nk Cells and Extrathymic T Cells)mentioning

confidence: 84%

Erythropoietin induces the expansion of c‐kit+ progenitors for myeloid and erythroid cells, but not for lymphoid cells, in the bone marrow and liver

Koya

Narita

Honda

et al. 1999

European J of Haematology

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In humans, the numbers of erythrocytes and granulocytes, but not that of lymphocytes, tend to increase in parallel. To determine the mechanism, we investigated how the administration of erythropoietin induces the expansion of erythroid cells and other lineage cells in the bone marrow, liver, and other organs of mice. When mice were injected twice (days 1 and 2) with erythropoietin at a dose of 20 or 200 IU/day/mouse, a prominent expansion of TER119+ (erythroid cells) and Gr‐1high cells (granulocytes) occurred in the liver, spleen, and bone marrow day 3 after the initial injection. On the other hand, lymphoid cells, including NK cells, extrathymic T cells, and conventional T cells, did not expand. In parallel with the expansion of erythroid cells and granulocytes, the levels of c‐kit+Lin− cells increased in the liver and bone marrow. Despite the increase in the proportion of c‐kit +Lin− cells, the generation of lymphocytes (e.g., T cells) decreased when such bone marrow cells were injected to scid mice. These results suggest that erythropoietin has the ability to induce the expansion of not only erythroid cells but also granulocytes in the liver, spleen, and bone marrow. Furthermore, c‐kit+ progenitors which may commit themselves to erythroid and myeloid cells, but not to lymphoid cells, were also activated in the liver and bone marrow of mice treated with erythropoietin.

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“…GM-CSF was reported to be a critical molecule for the initiation of TCR gene rearrangement in V␣14 NKT cells during the development of these lymphocytes (22). It was reported that mRNA of the mouse G-CSF receptor was expressed in mouse NKT cells (23). They did not restore the low proliferative response of V␣24 NKT cells from cancer patients excluding G-CSF (Fig.…”

Section: Discussionmentioning

confidence: 94%

Impaired Proliferative Response of Vα24 NKT Cells from Cancer Patients Against α-Galactosylceramide

Yanagisawa

Seino

Ishikawa

et al. 2002

The Journal of Immunology

123

100

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Human invariant Vα24+ NKT cells are a relatively new subpopulation of lymphocytes. It has been reported that Vα24 NKT cells are significantly involved in some human diseases. We have evaluated the number and function of Vα24 NKT cells in both healthy volunteers and cancer patients. In this study we found that Vα24 NKT cells in unfractionated PBMCs obtained from cancer patients did not respond efficiently to α-galactosylceramide (α-GalCer) in vitro. Thus, their proportion after stimulation with α-GalCer was smaller than that found in healthy volunteers. However, the cancer patients’ Vα24 NKT cells retained cytotoxic activity against malignant target cells, and they could efficiently proliferate to α-GalCer when fractionated by sorting. Furthermore, we found that addition of G-CSF to the culture could restore the low proliferative response of Vα24 NKT cells from cancer patients. These results suggest that some functions of NKT cells in cancer patients are impaired, and this observation carries significant implications for immunotherapy-based cancer treatments.

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Expansion of an Unusual Population of Gr-1+CD3intCells in the Lymph Nodes and Other Peripheral Organs of Mice Carrying thelprGene

Cited by 8 publications

References 22 publications

Fas ligand (gld)- and Fas (lpr)-deficient mice do not show alterations in the extravasation or apoptosis of inflammatory neutrophils

Fas ligand (gld)- and Fas (lpr)-deficient mice do not show alterations in the extravasation or apoptosis of inflammatory neutrophils

Erythropoietin induces the expansion of c‐kit+ progenitors for myeloid and erythroid cells, but not for lymphoid cells, in the bone marrow and liver

Impaired Proliferative Response of Vα24 NKT Cells from Cancer Patients Against α-Galactosylceramide

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